Estimating bacteria diversity in different organs of nine species of mosquito by next generation sequencing

Mancini, Maria Vittoria; Damiani, Claudia; Accoti, Anastasia; Tallarita, Mario; Nunzi, E.; Cappelli, Alessia; Bozic, Jovana; Catanzani, Riccardo; Rossi, Paolo; Valzano, Matteo; Serrao, Aurelio; Ricci, Irene; Spaccapelo, Roberta; Favia, Guido

doi:10.1186/s12866-018-1266-9

Cited by 98 publications

(137 citation statements)

References 42 publications

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“…This technique offers the advantage of detecting both culturable and unculturable pathogenic and nonpathogenic microbes from a given DNA or RNA sample. As previously reported from previous metagenome studies on mosquitoes and similar arthropods or insects [37][38][39], Fig. 1a shows that the phyla Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes were detected from the mosquito groups tested.…”

Section: Discussionsupporting

confidence: 81%

Laboratory colonization by Dirofilaria immitis alters the microbiome of female Aedes aegypti mosquitoes

Adegoke

Neff

Geary

et al. 2020

Preprint

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Abstract Background: The ability of blood-feeding arthropods to successfully acquire and transmit pathogens of medical and veterinary importance has been shown to be interfered with, or enhanced by, the arthropod’s native microbiome. Mosquitoes transmit viruses, protozoan and filarial nematodes, the majority of which contribute to the 17% of infectious disease cases worldwide. Dirofilaria immitis, a mosquito-transmitted filarial nematodes of dogs and cats, is vectored by several mosquito species including Aedes aegypti.Methods: In this study, we investigated the impact of D. immitis colonization on the microbiome of laboratory reared female Ae. aegypti. Metagenomic analysis of the V3-V4 variable region of the microbial 16S RNA gene was used for identification of the microbial differences down to species level.Results: We generated a total of 1068 OTUs representing 16 phyla, 181 genera and 271 bacterial species. Overall, in order of abundance, Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes were the most represented phylum with D. immitis-infected mosquitoes having more of Proteobacteria (71%) than uninfected mosquitoes (56.9%). An interesting finding in this study is the detection of Klebsiella oxytoca in relatively similar abundance in infected and uninfected mosquitoes, suggesting a possible endosymbiotic relationship, and has been previously shown to indirectly compete for nutrients with fungi on domestic housefly eggs and larvae. While D. immitis colonization has no effect on the overall species richness, we identified significant differences in the composition of selected bacterial genera and phyla between the two groups. We also reported distinct compositional and phylogenetic differences in the individual bacterial species when commonly identified bacteria were compared.Conclusions: To the best of our knowledge, this is the first study to understand the impact of a filarial infection on the microbiome of its mosquito vector. Further studies are required to identify bacteria species that could play an important role in the mosquito biology. While the microbiome composition of Ae. aegypti mosquito have been previously reported, our study shows that in an effort to establish itself, a filarial nematode modifies and alters the overall microbial diversity within its mosquito host.

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Section: Discussionsupporting

confidence: 81%

Laboratory colonization by Dirofilaria immitis alters the microbiome of female Aedes aegypti mosquitoes

Adegoke

Neff

Geary

et al. 2020

Preprint

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“…gambiae and An. coluzii [27]. To the best of our knowledge, this is the first study to demonstrate E. anophelis in the saliva of Aedes mosquitoes.…”

Section: Discussionmentioning

confidence: 98%

Potential for transmission ofElizabethkingia anophelisbyAedes albopictusand the role of microbial interactions in Zika virus competence

Onyango

Payne

Stout

et al. 2019

Preprint

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Elizabethkingia anophelis has been the cause of four outbreaks with significant morbidity and mortality. Its transmission routes remain unknown and no point source of infection has been identified. Here we show that E. anophelis can be found in the saliva of Aedes mosquitoes, suggesting the novel possibility of vector-borne transmission of this bacterium. We additionally characterized diverse microbial communities in Aedes midguts, salivary glands and saliva. To the best of our knowledge, this represents the first description of the microbiome of Aedes saliva. Further, we demonstrate that increased abundance of E. anophelis is associated with decreased susceptibility and replication of Zika virus (ZIKV) in the midgut of Aedes mosquitoes, suggesting a novel transmission barrier for arboviruses transmitted by Aedes mosquitoes. Together, these results demonstrate the complex relationships between the mosquito, the midgut microbial community and arboviruses and offer insights into the epidemiology and control of emerging bacterial and viral pathogens.Author SummaryElizabethkingia anophelis has in the recent past caused outbreaks different parts of the world resulting both in morbidity and mortality. Until now, to the best of our knowledge, no study has been able to demonstrate that this bacterium can be transmitted by mosquitoes. We have demonstrated for the first time that Elizabethkingia anophelis is present in the saliva of both infected and non-infected Aedes mosquitoes. Further, we have shown that it confers an inhibitory effect on Zika virus establishment in the midguts of Aedes mosquitoes. Together, these results potentially display the potential for vector borne transmission of E. anophelis as well as a novel transmission barrier of ZIKV. Lastly, we have for the first time characterized salivary microbes of Aedes mosquitoes necessitating the investigation of the impact of salivary microbes in severity of disease in vertebrate hosts.

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“…We directed this research to examine the distribution of bacterial communities and the impact of water temperature. To determine the bacterial distribution through the region, Illumina next-generation sequencing analyses [23][24][25] have been used to identify bacteria from water samples in the study region.…”

Section: Introductionmentioning

confidence: 99%

Spatial Modelling of Bacterial Diversity over the Selected Regions in Bangladesh by Next-Generation Sequencing: Role of Water Temperature

et al. 2020

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In this study, a spatial model has been developed to investigate the role of water temperature to the distribution of bacteria over the selected regions in the Bay of Bengal, located in the southern region of Bangladesh using next-generation sequencing. Bacterial concentration, quantitative polymerase chain reactions, and sequencing were performed on water samples and identified Acidobacteria, Actinobacteria, Bacteroidetes, Chlorobi, Chloroflexi, Cyanobacteria, Firmicutes, Nitrospirae, Planctomycetes, Proteobacteria, and Verrucomicrobia. The spatial model tessellated the parts of the Bay of Bengal with hexagons and analyzed the relationship between the distribution of bacteria and water temperature. A geographically weighted regression was used to observe whether water temperature contributed strongly or weakly to the distribution of bacteria. The residuals were examined to assess the model's fitness. The spatial model has the potential to predict the bacterial diversity in the selected regions of Bangladesh.

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Estimating bacteria diversity in different organs of nine species of mosquito by next generation sequencing

Cited by 98 publications

References 42 publications

Laboratory colonization by Dirofilaria immitis alters the microbiome of female Aedes aegypti mosquitoes

Laboratory colonization by Dirofilaria immitis alters the microbiome of female Aedes aegypti mosquitoes

Potential for transmission ofElizabethkingia anophelisbyAedes albopictusand the role of microbial interactions in Zika virus competence

Spatial Modelling of Bacterial Diversity over the Selected Regions in Bangladesh by Next-Generation Sequencing: Role of Water Temperature

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