Estimates of Within-Subject Biological Variation of Protein C, Antithrombin, Protein S Free, Protein S Activity, and Activated Protein C Resistance in Pregnant Women

Kristoffersen, Ann Helen; Petersen, Per Hyltoft; Røraas, Thomas; Sandberg, Sverre

doi:10.1373/clinchem.2016.265900

Cited by 18 publications

(13 citation statements)

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“…Other characteristics of the participating women and their health status during pregnancy can be found in earlier papers. 19–21 Study participants were recruited by an advertisement in the local newspaper and on the webpage of Haukeland University Hospital. The Regional Committee of Medical Ethics of Western Norway approved the study (Project number 3.2006.1869/REK Vest 2010/221), and the women gave informed consent to participate.…”

Section: Methodsmentioning

confidence: 99%

“…24,25 Confidence intervals (95%) were calculated according to Burdick and Graybill. 26 Testing for variance homogeneity was performed as described in detail in earlier publications 19–21 and was performed by Bartlett’s test (statistical significance, P < 0.01). 27 Variance homogeneity was illustrated on a rankit scale where the cumulated ranked coefficients of variation were plotted as fractions on the ordinate as function of the personal values of the variation, SD P or CV P .…”

Section: Statisticsmentioning

confidence: 99%

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Concentration of fibrin monomer in pregnancy and during the postpartum period

et al. 2019

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Background D-dimer increases during pregnancy and is problematic to use in the diagnosis of venous thromboembolism. Fibrin monomer represents an alternative biomarker for venous thromboembolism. However, to be useful in pregnancy, the fibrin monomer concentration should be stable throughout pregnancy and during postpartum. Methods To describe the course of fibrin monomer concentration during pregnancy and the postpartum period in healthy pregnant women and to compare their within-subject biological variation (CVI) with non-pregnant women. Blood samples were obtained every fourth week during pregnancy and three samples after delivery in 20 healthy women and every fourth week during a 40-week period in 19 healthy non-pregnant women. Fibrin monomer (STA Liatest FM, Stago) was analysed in duplicates for all samples. Concentrations of fibrin monomer in pregnant and non-pregnant women were compared and the CVI for fibrin monomer was calculated. Results The median fibrin monomer concentration in pregnant women was 6.2 mg/L (2.5 and 97.5 percentiles 3.7–10.8 mg/L) and in non-pregnant women 4.8 mg/L (3.6–8.2) ( P < 0.01). The fibrin monomer concentration was relatively stable during pregnancy, although a few unexplained high fibrin monomer concentrations were found during pregnancy/postpartum. Fibrin monomer CVI in pregnancy and postpartum was 20.6% (95% CI 18.3, 23.5) and in non-pregnant 16.1% (13.7, 18.9). Conclusions For clinical purposes, fibrin monomer concentration can be considered stable during pregnancy, although it is slightly higher than in non-pregnant women. Pregnant and non-pregnant women have the same CVI. The suitability of fibrin monomer in venous thromboembolism diagnosis in pregnant women should be validated in further studies.

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Section: Methodsmentioning

confidence: 99%

Section: Statisticsmentioning

confidence: 99%

Concentration of fibrin monomer in pregnancy and during the postpartum period

et al. 2019

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“…PC, PS, and ATIII are three vitamin K-dependent anticoagulant proteins that are key roles in the coagulation regulation [27] . A previous study showed that PS was signi cantly decreased during pregnancy, ATIII was slightly decreased, but the PC expression was largely variated [28] . In the current study, signi cantly differences were identi ed in the expression of serum PC and PS, but not ATIII, between BC and HC women.…”

Section: Discussionmentioning

confidence: 92%

Expression and Diagnostic Value of Serum Protein Z, Protein Z-dependent Protease Inhibitor and Coagulation-related Indicators in Foetal Growth Restriction.

Luo

Huang

et al. 2020

Preprint

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Objective: This study aimed to explore the expression and associations between levels of serum protein Z (PZ), protein Z-dependent protease inhibitor (ZPI), and coagulation-related indicators and foetal growth restriction (FGR).Methods: A total of 50 pregnant women with FGR, 50 healthy pregnant women (Healthy control, HC), and 30 childbearing-age women without pregnancy group (Blank control, BC) were collected in this study. The serum PZ, ZPI, protein C (PC), protein C (PS), and antithrombin III (ATIII) levels of women in each group were determined and compared. Moreover, correlations between these indices and FGR were assessed using Spearman analysis and diagnostic values of these indices for FGR were evaluated using the receiver operating characteristics (ROC) curves.Results: Serum levels of PZ, ZPI, PC, PS, and ATIII were significantly downregulated in the HC and FGR groups compared with the BC group (P <0.001), while PZ, ZPI, PS, and ATIII levels in the FGR groups were significantly lower than that in the HC group (P <0.01). PZ serum concentration was positively correlated with ZPI concentration in the HC and FGR groups, and PZ and ZPI levels were negatively correlated with FGR (r = -0.198, r = -0.247, P <0.05). Combined of PZ, ZPI, PS, and ATIII performed a better value than each of them with AUC of 0.935 (95% CI=0.890-0.981), a sensitivity of 0.920, and a specificity of 0.800.Conclusion: Serum PZ, ZPI, PS, and ATIII were significantly decreased in pregnant women with FGR and could be used for FGR screening in pregnant women.

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“…In this study, the levels of free protein S were mea-sured as a percentile, and it is stated that 50~150% is normal range as a reference (Kristoffersen et al 2017). Twenty-one patients had low levels of plasma free protein S (< 50%) which means deficient level ( Table 2).…”

Section: Clinical Features Of Patients With Low Plasma Free Protein Smentioning

confidence: 86%

Plasma Free Protein S Is Correlated with Disease Activity, but not with Subclinical Atherosclerosis among Patients with Systemic Lupus Erythematosus: A Cross-Sectional Study

Jung

Lee

Kim

et al. 2019

Tohoku J. Exp. Med.

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A defect in clearance of apoptotic materials is pivotal in the pathogenesis of systemic lupus erythematosus (SLE). Protein S participates in the removal of apoptotic remnants and the anticoagulation pathway. The aim of the study was to clarify the relationship between plasma levels of free protein S and the disease activity or subclinical atherosclerosis in SLE. Free protein S was measured by an enzyme-linked immunosorbent assay, and patients were classified into two groups of free protein S levels: low (< 50%) and normal (≥ 50%). One hundred-eleven Korean female patients with SLE were enrolled, and the levels of free protein S were 67.4 ± 19.7%. Carotid plaque was detected in 25 (22.5%) patients. Twenty-one patients with low free protein S had lower hemoglobin (11.4

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Estimates of Within-Subject Biological Variation of Protein C, Antithrombin, Protein S Free, Protein S Activity, and Activated Protein C Resistance in Pregnant Women

Abstract: Transformation of coagulation parameters in healthy pregnancies to lnMoM is a tool to establish a kind of steady state. Although there is a physiological change in PC, AT, and PS free and PS activity during pregnancy, the CV was comparable with the CV of nonpregnant women.

Cited by 18 publications

References 30 publications

Concentration of fibrin monomer in pregnancy and during the postpartum period

Concentration of fibrin monomer in pregnancy and during the postpartum period

Expression and Diagnostic Value of Serum Protein Z, Protein Z-dependent Protease Inhibitor and Coagulation-related Indicators in Foetal Growth Restriction.

Plasma Free Protein S Is Correlated with Disease Activity, but not with Subclinical Atherosclerosis among Patients with Systemic Lupus Erythematosus: A Cross-Sectional Study

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