Estimate of symbiotically fixed nitrogen in field grown soybeans using variations in15N Natural abundance

Amarger, Noëlle; Mariotti, André; Mariotti, François; Dürr, J.; Bourguignon, Claude; Lagacherie, Bernard

doi:10.1007/bf02184565

Cited by 143 publications

(77 citation statements)

References 5 publications

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“…However, little is known about the effect of nodulation on nitrate utilization. Non-nodulated soybeans absorbed more nitrate than nodulated plants (Wych & Rains, 1978), and nodulated soybeans accumulated smaller amounts of than non-nodulated ones (Amarger et al, 1979). NRA was depressed in the leaves of nodulated soybeans in comparison with those of non-nodulated plants (Conejero, Tirado & Robin, 1986).…”

Section: Introductionmentioning

confidence: 84%

Effect of Nodulation on the Expression of Nitrate Reductase Activity in Pea Cultivars

et al. 1987

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SUMMARYNitrate reductase activity (NRA) was measured in leaves, stems, roots and nodules of four cultivars of Pisum sativum L. supplied with 2 mM KNOg and nodulated by selected Rhizobium leguminosarum strains. As a control, non-nodulated (uninoculated) plants were grown in the same nutrient medium. NRA was determined by an in vitro assay; it varied widely among strains and cultivars. Although differences in tissue NRA in nodulated plants were mainly related to cultivar genotype, nodulation much affected the distribution of NRA within the plant. There was also some cultivar x bacteria strain effect. Non-nodulated plants had higher leaf and root NRA than nodulated cultivars, but nodulated plants had twice as high stem NRA as nonnodulated plants. These differences correlated with nitrate concentrations which were depressed in leaves and roots, and increased in stems, of nodulated plants Nitrate accumulation was increased in leaves, stems and roots of nodulated plants in comparison with non-nodulated ones. There were no differences in the root to shoot ratio between nodulated and non-nodulated plants. The relationships between tissue nitrate reduction and nodulation are discussed.

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Section: Introductionmentioning

confidence: 84%

Effect of Nodulation on the Expression of Nitrate Reductase Activity in Pea Cultivars

et al. 1987

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“…Most measurements of isotopic fractionation associated with N2 fixation have shown this effect to be, in general, very small (1-3, 7, 8). Amarger et al (2) measured the isotope effect on N2 fixation by four species of soybeans to be about -I%o. For the variety of soybeans used in these experiments, we have measured the isotope effect on N2 fixation to be +I%o (8).…”

Section: Methodsmentioning

confidence: 99%

Estimates of N₂ Fixation Based on Differences in the Natural Abundance of ¹⁵N in Nodulating and Nonnodulating Isolines of Soybeans

1980

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Estimates of the contribution of biologically fixed N to the total N of nodulating soybeans (Glycine max (L) Merrill, variety Harosoy) grown under a variety of conditions were made from: (a) differences in N yield between nodulating and nonnodulating isolines; and (b) differences in 15N abundance between the two isolines. For plants grown in a greenhouse in nutrient-poor soil, both estimates showed a high level of N2 fixation; from 58 to 89% N fixed by differences in N yield and from 51 to 95% by differences in "5N abundance. Decreasing contributions of fixed N were estimated by both methods with increasing levels of added N03-. Results of field experiments carried out over two years on an unamended highly fertile midwestern soil showed a modest level of N2 fixation by both methods (7.3 to 51% by differences in N yield, and 5.4 to 46% by differences in 15N abundance). When the soil was amended with ground corn cobs, both methods showed higher contributions of fixed N. The two methods of estimating N2 fixation gave similar results. Both appear to be semiquantitative and the standard errors of the estimates were about the same (6% on the average).A number of investigators (1-4, 9, 10, 11, 14) have reported small differences in the 15N abundance between N2-fixing and non-N2-fixing plants, with the 15N abundance in N2-fixing plants being consistently lower than in nonfixing plants. These investigators have suggested that this difference could be used to indicate the contribution of atmospheric N2 to the total N of the N2-fixing plants. Such a method requires that: (a) there be a measurable difference in the "5N abundance between the two major sources of N to N2 fixing plants (atmospheric N2 and soil-derived N); and (b) this difference be maintained after alteration of 15N abundance resulting from isotopic discrimination on uptake of N. If portions of the plant, rather than the entire plant, are sampled, the method also requires that isotopic discrimination associated with metabolic processes, and subsequent transport of isotopically altered N compounds, does not result in large distortions of the original "5N abundance in the sources of the N.In applying the natural "5N abundance method, the 15N abundance in non-N2-fixing plants is used as a measure of the 15N content of soil-derived N, after it has undergone any fractionation that may occur during N transformations in the soil and on uptake into the plant. The 1 N abundance of the N2-fixing plant should

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“…The fraction of N in Alnus trees derived from N 2 -fixation (f n ) was calculated as (Amarger et al, 1979;Cadish et al, 2000):…”

Section: Isotope Analysesmentioning

confidence: 99%

Free atmospheric CO<sub>2</sub> enrichment increased above ground biomass but did not affect symbiotic N<sub>2</sub>-fixation and soil carbon dynamics in a mixed deciduous stand in Wales

et al. 2011

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Abstract. Through increases in net primary production (NPP), elevated CO 2 is hypothesized to increase the amount of plant litter entering the soil. The fate of this extra carbon on the forest floor or in mineral soil is currently not clear. Moreover, increased rates of NPP can be maintained only if forests can escape nitrogen limitation. In a Free atmospheric CO 2 Enrichment (FACE) experiment near Bangor, Wales, 4 ambient and 4 elevated [CO 2 ] plots were planted with patches of Betula pendula, Alnus glutinosa and Fagus sylvatica on a former arable field. After 4 years, biomass averaged for the 3 species was 5497 (se 270) g m −2 in ambient and 6450 (se 130) g m −2 in elevated [CO 2 ] plots, a significant increase of 17% (P = 0.018). During that time, only a shallow L forest floor litter layer had formed due to intensive bioturbation. Total soil C and N contents increased irrespective of treatment and species as a result of afforestation. We could not detect an additional C sink in the soil, nor were soil C stabilization processes affected by elevated [CO 2 ]. We observed a decrease of leaf N content in Betula and Alnus under elevated [CO 2 ], while the soil C/N ratio decreased regardless of CO 2 treatment. The ratio of N taken up from the soil and by N 2 -fixation in Alnus was not affected by elevated [CO 2 ]. We infer that increased nitrogen use efficiency is the mechanism by which increased NPP is sustained under elevated [CO 2 ] at this site.

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Estimate of symbiotically fixed nitrogen in field grown soybeans using variations in15N Natural abundance

Cited by 143 publications

References 5 publications

Effect of Nodulation on the Expression of Nitrate Reductase Activity in Pea Cultivars

Effect of Nodulation on the Expression of Nitrate Reductase Activity in Pea Cultivars

Estimates of N₂ Fixation Based on Differences in the Natural Abundance of ¹⁵N in Nodulating and Nonnodulating Isolines of Soybeans

Free atmospheric CO<sub>2</sub> enrichment increased above ground biomass but did not affect symbiotic N<sub>2</sub>-fixation and soil carbon dynamics in a mixed deciduous stand in Wales

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Estimate of symbiotically fixed nitrogen in field grown soybeans using variations in15N Natural abundance

Cited by 143 publications

References 5 publications

Effect of Nodulation on the Expression of Nitrate Reductase Activity in Pea Cultivars

Effect of Nodulation on the Expression of Nitrate Reductase Activity in Pea Cultivars

Estimates of N2 Fixation Based on Differences in the Natural Abundance of 15N in Nodulating and Nonnodulating Isolines of Soybeans

Free atmospheric CO&lt;sub&gt;2&lt;/sub&gt; enrichment increased above ground biomass but did not affect symbiotic N&lt;sub&gt;2&lt;/sub&gt;-fixation and soil carbon dynamics in a mixed deciduous stand in Wales

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Estimates of N₂ Fixation Based on Differences in the Natural Abundance of ¹⁵N in Nodulating and Nonnodulating Isolines of Soybeans

Free atmospheric CO<sub>2</sub> enrichment increased above ground biomass but did not affect symbiotic N<sub>2</sub>-fixation and soil carbon dynamics in a mixed deciduous stand in Wales