In nuclear power plants, due to the special working environment, the status of the fuel rods will be changed by the influence of radiation. And these changes, such as diameter expansion, will cause damage to the fuel rods and even affect the safe operation of nuclear power plants. Therefore, it is necessary to accurate diameter measurement for nuclear fuel rods. This paper aims to solve the measurement problem of fuel rods, and proposes a measurement technique based on image processing. In the proposed scheme, based on Zhang's method, tangential distortion is considered to be an affecting factor at first to improve the accuracy of camera calibration. Then, a particle swarm algorithm with all-parameter adaptive mutation method is proposed for the optimization of camera parameters. Finally, a simple and convenient method to calculate pixel equivalent is designed by considering the feature extraction of corner coordinates of subpixel to accurately obtain pixel equivalents. The effectiveness of this measurement scheme is highlighted on a measuring platform. The relative error of the proposed method reaches 0.041%, and RMSE reaches 4.16 μm. Experimental results demonstrate that this approach is efficient for highaccuracy diameter measurement of fuel rods. INDEX TERMS Camera calibration, diameter measurement, image processing, pixel equivalent, parameters optimization, tangential distortion.