Estetrol attenuates neonatal hypoxic–ischemic brain injury

Tskitishvili, Ekaterine; Nisolle, Michelle; Munaut, Carine; Péqueux, Christel; Gérard, Céline; Noël, Agnès; Foidart, Jean‐Michel

doi:10.1016/j.expneurol.2014.07.015

Cited by 25 publications

(29 citation statements)

References 65 publications

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“…As it was shown from our previous study [33], E4 positively affected the expression of a marker of neurogenesis (DCX), suggesting upregulation of neurogenesis, possibly through estrogen receptors. Our current study is in concert with the previous one and, moreover, it shows that combined use of E4 with P4 also affects neurogenesis suggesting that neurogenesis might be upregulated not only through estrogen receptors but through progesterone receptors as well, though involvement of some other mechanisms is also plausible.…”

Section: Discussionsupporting

confidence: 54%

“…We prepared primary hippocampal neuronal cultures from newborn (P0) Sprague-Dawley (SD) rat pups brains according to the recently published protocols [98, 99] which we have used in our previous study [33]. Briefly, brains were dissected to separate hippocampus region.…”

Section: Methodsmentioning

confidence: 99%

“…Successful concentrations (100nM of E2 and 1mM of P4) were further considered for combination studies with E4 (Pantarhei Bioscience, Zeist, The Netherlands) in primary hippocampal cell cultures. We used the same concentrations of E4 as in our previous in vitro studies [33]. To study an antioxidative and neuroprotective effects of E4 in combination with other steroids on primary hippocampal cell cultures at day 7 in vitro (DIV), they were stimulated with 100 μM of H202 for 30 min followed by treatment for 1 h either with 650μM, 3.25mM and 6.5mM E4 alone or in combination with 100nM E2 and/or 1mM P4 as followed: E4+100nM E2, E4+1mM P4, E4+100nM E2+1mM P4.…”

Section: Methodsmentioning

confidence: 99%

“…Our recent studies showed that E4 has very good antioxidant, neuroprotective, neuro- and angiogenic properties [33]. It was also shown that this steroid modulates the production of allopregnanolone in various brain regions, and upregulates expression of β-endorphin in the nervous system [34, 35].…”

Section: Introductionmentioning

confidence: 99%

“…As a consequence, E4 has biological activities distinct from E2, depending on the tissues and cells, and the selective binding to the nuclear/membrane form of ERα [36]. Only limited data show the significance of separately used estrogens and progesterone in attenuation of neonatal hypoxic-ischemic encephalopathy [33, 37- 41] based on a model of HIE in a 7-day-old immature rats [42]. …”

Section: Introductionmentioning

confidence: 99%

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Use of estetrol with other steroids for attenuation of neonatal hypoxic-Ischemic brain injury: to combine or not to combine?

et al. 2016

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Estetrol (E4), estradiol (E2) and progesterone (P4) have important antioxidative and neuroprotective effects in neuronal system. We aimed to study the consequence of combined steroid therapy in neonatal hypoxic-ischemic encephalopathy (HIE). In vitro the effect of E4 combined with other steroids on oxidative stress and the cell viability in primary hippocampal cultures was evaluated by lactate dehydrogenase and cell survival assays. In vivo neuroprotective and therapeutic efficacy of E4 combined with other steroids was studied in HIE model of immature rats. The rat pups rectal temperature, body and brain weights were evaluated. The hippocampus and the cortex were investigated by histo/immunohistochemistry: intact cell number counting, expressions of markers for early gray matter lose, neuro- and angiogenesis were studied. Glial fibrillary acidic protein was evaluated by ELISA in blood samples. In vitro E4 and combinations of high doses of E4 with P4 and/or E2 significantly diminished the LDH activity and upregulated the cell survival.In vivopretreatment or treatment by different combinations of E4 with other steroids had unalike effects on body and brain weight, neuro- and angiogenesis, and GFAP expression in blood. The combined use of E4 with other steroids has no benefit over the single use of E4.

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Section: Discussionsupporting

confidence: 54%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Use of estetrol with other steroids for attenuation of neonatal hypoxic-Ischemic brain injury: to combine or not to combine?

et al. 2016

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Estetrol and Its Effects on the Damaged Brain

Tskitishvili

Foidart

2019

ISGE Series

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Estetrol and Mammary Gland: Friends or Foes?

Gallez

Silva

Wuidar

et al. 2021

J Mammary Gland Biol Neoplasia

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Estrogens have pleiotropic effects on many reproductive and non-reproductive tissues and organs including the mammary gland, uterus, ovaries, vagina, and endothelium. Estrogen receptor α functions as the principal mediator of estrogenic action in most of these tissues. Estetrol (E4) is a native fetal estrogen with selective tissue actions that is currently approved for use as the estrogen component in a combined oral contraceptive and is being developed as a menopause hormone therapy (MHT, also known as hormone replacement therapy). However, exogenous hormonal treatments, in particular MHTs, have been shown to promote the growth of preexisting breast cancers and are associated with a variable risk of breast cancer depending on the treatment modality. Therefore, evaluating the safety of E4-based formulations on the breast forms a crucial part of the clinical development process. This review highlights preclinical and clinical studies that have assessed the effects of E4 and E4-progestogen combinations on the mammary gland and breast cancer, focusing in particular on the estrogenic and anti-estrogenic properties of E4. We discuss the potential advantages of E4 over current available estrogen-formulations as a contraceptive and for the treatment of symptoms due to menopause. We also consider the potential of E4 for the treatment of endocrine-resistant breast cancer.

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Estetrol attenuates neonatal hypoxic–ischemic brain injury

Cited by 25 publications

References 65 publications

Use of estetrol with other steroids for attenuation of neonatal hypoxic-Ischemic brain injury: to combine or not to combine?

Use of estetrol with other steroids for attenuation of neonatal hypoxic-Ischemic brain injury: to combine or not to combine?

Estetrol and Its Effects on the Damaged Brain

Estetrol and Mammary Gland: Friends or Foes?

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