Esterases in histochemistry and ultrahistochemistry

Deimling, Otto von; Böcking, Alfred

doi:10.1007/bf01003814

Cited by 58 publications

(12 citation statements)

References 190 publications

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“…The strong intensity of the enzyme suggests that this segment of the intestine may be important in the hydrolysis of carboxylic esters, which occurs mainly during lipid and carbohydrate metabolism (Deimling and B6cking 1976). However, these processes may not occur in the anterior intestine where activity of nonspecific esterase is absent.…”

Section: Discussionmentioning

confidence: 99%

“…The functional development of the pyloric intestine is supported by an increase in the activity of nonspecific esterase associated with microsomes of endoplasmic reticulum as well as with various organelles such as Golgi apparatus, mitochondria and lysosomes (Deimling and B6cking 1976). The strong intensity of the enzyme suggests that this segment of the intestine may be important in the hydrolysis of carboxylic esters, which occurs mainly during lipid and carbohydrate metabolism (Deimling and B6cking 1976).…”

Section: Discussionmentioning

confidence: 99%

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Histological and histochemical changes in the digestive tract of white sturgeon larvae during ontogeny

Gawlicka

Teh

Hung

et al. 1995

Fish Physiol Biochem

103

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Ontogenetic changes in digestive tract histology and digestive enzyme histochemistry were investigated 11 to 36 days post-hatch in white sturgeon Acipenser transmontanus larvae. From initiation of exogenous feeding (12 days post-hatch), larvae were fed a commercial salmonid diet for the ensuing 24 days. The digestive system of white sturgeon displayed a high degree of morphologic organization and functionality at the onset of exogenous feeding. An enhancement of digestive capacities occurred with transition to active feeding. On day 2 of feeding, there was a clear increase of alkaline phosphatase, aminopeptidase M, dipeptidyl peptidase IV, and γ-glutamyl transpeptidase activity in the brush border of the spiral intestine. This strong activity is an apparent confirmation of the importance of this segment of the intestine for protein digestion and nutrient absorption. The functional development of the pyloric intestine occurred on day 4 and was concomitant with an increase in the activity of brush border and cytoplasmic enzymes such as acetylcholinesterase, dipeptidyl peptidase II, α- and β-galactosidases. The absence of acetylcholinesterase, lactase, nonspecific esterase, and weak activity of exopeptidases and alkaline phosphatase in the anterior intestine suggests that this segment of the intestine may be less important in nutrient absorption than the pyloric and spiral intestines. The observed quantitative and qualitative differences in enzyme activity along the intestine indicate a high degree of specialization of each segment for specific digestive and absorptive processes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Histological and histochemical changes in the digestive tract of white sturgeon larvae during ontogeny

Gawlicka

Teh

Hung

et al. 1995

Fish Physiol Biochem

103

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“…This preferential location was found in many tissues and has been revealed with different substrates (cf. Deimling and Bocking, 1976). In the mouse epididymis, no difference was seen in staining patterns of the ER, which can 9.…”

Section: Discussionmentioning

confidence: 90%

“…Thus the substrate chosen for the ultrastructural demonstration of this enzyme was 5-bromo-indoxyl-acetate, one of the more selective substrates used to study the specific hydrolysis patterns of carboxylic esters (Deimling and Bocking, 1976). Apparently incubation duration (70 or 90 minutes) did not influence the enzyme distribution in ultrathin sections.…”

Section: Discussionmentioning

confidence: 99%

“…These variations are probably the expression of different functional states. They may correspond to quantitative changes, or to a different subcellular distribution in cell compartments as shown in other organs (Deimling and Bocking, 1976). For example, in the interstitial cells or prepubertal mouse testis, esterase is exclusively associated with lipid droplets whereas in the Leydig cells of testosterone-producing adult testis, the esterase reaction is predominantly in mitochondria and at cellular membranes.…”

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confidence: 97%

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Subcellular distribution of the nonspecific esterase in the mouse epididymis with special reference to regional differences

Abou‐Haïla¹

1986

Anat. Rec.

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The subcellular distribution of esterases was studied in mouse epididymis by using 5-bromo-indoxyl-acetate as a substrate. In all the cells of the duct, a low level of esterase activity was detected except in one of the five segments of the head--segment IV; in one of the three types of apical cells--the "prominent cells"; and in the "clear cells" scattered in the middle and distal parts. In these cells, the intensity of the reaction was high. The reaction product was consistently found in the endoplasmic reticulum and was more abundant in cells showing a high level of activity than in others. In cells with low esterase activity, the reaction was mainly restricted to this organelle. In highly active cells, the spectrum of subcellular locations was selectively enlarged and esterase was demonstrated in almost all cell compartments, including the cell membrane, nuclear envelope, mitochondria, lytic structures, and, more rarely in the Golgi apparatus or microvilli. These locations were dependent on cell type. A weak enzyme activity also appeared on mature spermatozoa.

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Micro two‐dimensional gel electrophoresis of serum and testis esterases from different strains of mice

Abou-Haïla

1990

Electrophoresis

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Two-dimensional electrophoresis with time-dependent polyacrylamide gradient gel electrophoresis (PAGGE) in the second dimension was applied to the separation of native molecular forms of esterases from serum and testis of four strains of mice (C57BL/6J, Swiss OF1, F1 hybrid derived from these two populations and Tfm). In Phast System, a modified pH 3-9 gradient, a linear 8-25% gel gradient and a migration time corresponding to 300 Vh, were found to provide the best conditions for esterase analysis. About 70 esterase-active fractions could be separated with good reproducibility. The variants were characterized by their pI (3.9-7.35), their relative mobility and the visual estimation of their susceptibility towards neuraminidase and different esterase inhibitors. In the two tissues, the distribution of the esterase variants corresponded to a 50-500 kDa molecular mass range of calibration proteins, but most of the serum and testis-specific isoforms were confined to the 59-72 kDa range. All serum variants contained a terminal N-acetylneuraminic acid residue, whereas only the testicular esterases in common with those in serum appeared sensitive to neuraminidase. Cholinesterases with a low relative mobility and carboxylesterases with a high relative mobility were detected in serum, while carboxylesterases accounted for the greatest part in the testis which also contained cholinesterases and acetylesterases. Minor interspecies differences were found between C57BL/6J and Swiss OF1 esterases. The expression of two variants which differed between these two species seemed intermediate for the hybrid originating from these two populations. Two new spots were detected in the two-dimensional map of esterases from the strain bearing the Tfm mutation.

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Esterases in histochemistry and ultrahistochemistry

Cited by 58 publications

References 190 publications

Histological and histochemical changes in the digestive tract of white sturgeon larvae during ontogeny

Histological and histochemical changes in the digestive tract of white sturgeon larvae during ontogeny

Subcellular distribution of the nonspecific esterase in the mouse epididymis with special reference to regional differences

Micro two‐dimensional gel electrophoresis of serum and testis esterases from different strains of mice

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