Esterase Activity in Leukocytes Demonstrated by the Use of Naphthol as-D Chloroacetate Substrate

Abstract

Gouuiori (4) in 1953 pointed out that the substrate uuaphutiiol AS chboro:ocetate gave entirely olifferemut histochuemuuio'al pictures of estertose activity itu tissuo's thuauu the regulttr naphuthol aoetates, anb with this substrate hue miO)t('(l strikimug staining reaotions in normal and leukemic iueutrophuihie leukocyto's. Recently * This ituVe-sIigtotio)tt us-its uipporteol IIy a research grtomut (AI-30-l) 1571 from the Atomic Euuergy Conunuuis-siolnu tomud NIH granul C.Y. 4724. t Part lv sumtlllolrtcoh hI\-I he Larkimu Futmid Resetorchi FelloIsss4hip prolvioteot by the Guild of St. Luikc, Bo&lomi, Mtoss ochsumsetti4. Prcsenitcd tot I hue Tcmil Is Autmiuttol meetimug of the Histochemictol Society,

Immune functions of human synovial cells. phenotypic and t cell regulatory properties of macrophage‐like cells that express hla–dr

“…Frozen sections from normal and rheumatoid synovial tissue and cytocentrifuge preparations of cells obtained from normal and rheumatoid synovial specimens were investigated for the presence of acid alpha-naphtyl acetate esterase (ANAE) (22), naphthol AS-D acetate esterase (NASDAE), inhibitable by sodium fluoride (NaF) (23,24), and naphthol AS-D chloroacetate esterase (NASDCAE) (25). ATPase staining was performed on normal suspended synoviocytes (26).…”

Active Plasma Kallikrein Localizes to Mast Cells and Regulates Epithelial Cell Apoptosis, Adipocyte Differentiation, and Stromal Remodeling during Mammary Gland Involution

“…To visualize mast cells, frozen sections were air-dried for ϳ30 min, post-fixed in ice-cold acetone for 10 min, rinsed in PBS, and then stained in either Toluidine blue (a metachromatic dye for mast cells, made up as 0.1% Toluidine blue O (Sigma) in 1% sodium chloride, pH 2.3), Csaba stain (Alcian blue/Safranin O, an amine/heparin stain for mast cells, made up as 0.36% Alcian blue (Sigma), 0.018% Safranin O (Sigma) in acetate buffer, pH 1.42), or by using an enzymatic reaction with naphthol AS-D chloroacetate esterase (Sigma) to detect chymase activity (16), then counterstained with Gill's hematoxylin or DAPI. For inhibitor-localization, frozen sections were prepared as above, then incubated overnight with 50 nM ecotinPKal (10) biotinylated with Sulfo-NHS-LC-LC biotin (EZ-Link, Pierce), followed by 1-h incubation with Alexa 488-conjugated streptavidin (Invitrogen).…”

“…Plasma Kallikrein Specifically Localizes to Mast Cell Granules-To determine the subcellular localization of ecotinPKal in mast cells, we co-stained cells for chymase activity using naphthol chloroacetate esterase, a marker of mast cell granule chymase that appears red in both brightfield and fluorescent microscopy (16). PKal localized to the cytoplasmic granules of mast cells in wild-type mice (Fig.…”

The Value of CD34, Myeloperoxidase and Chloracetate Esterase (Leder) Stain in the Diagnosis of Granulocytic Sarcoma

“…Histochemical stain for the chloroacetate esterase (Leder) stain was performed. 30 Immunohistochemical stains were performed for CD3, CD20, myeloperoxidase (DAKO) and CD34 (Navocastra) on the above-mentioned selected slides. The immunoperoxidase technique with microwave antigen retrieval was used.…”