Establishment of uterine cell lines from p53-deficient mice

Hanazono, Makoto; Tomisawa, Hiroko; Tomooka, Yasuhiro; Hirabayashi, Kazuhiko; Aizawa, Shinichi

doi:10.1007/s11626-997-0121-3

Cited by 26 publications

(15 citation statements)

References 6 publications

(5 reference statements)

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“…Therefore, attempts have been made to establish immortalized MDF cells in order to analyze their differentiation potential. A deficiency in p53 is sufficient for the establishment of mouse clonal cell lines from various tissues (Hanazono et al 1997). HPV16E6 has been shown to abrogate the function of p53 and has the ability to immortalize various cell types (Fehrmann and Laimins 2003;Kiyono et al 1998).…”

Section: Discussionmentioning

confidence: 99%

Establishment of immortalized dental follicle cells for generating periodontal ligament in vivo

Yokoi

Saito

Kiyono³

et al. 2006

Cell Tissue Res

120

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The dental follicle is a mesenchymal tissue that surrounds the developing tooth germ. During tooth root formation, periodontal components, viz., cementum, periodontal ligament (PDL), and alveolar bone, are created by dental follicle progenitors. Here, we report the presence of PDL progenitors in mouse dental follicle (MDF) cells. MDF cells were obtained from mouse incisor tooth germs and immortalized by the expression of a mutant human papilloma virus type 16 E6 gene lacking the PDZ-domain-binding motif. MDF cells expressing the mutant E6 gene (MDF( E6-EGFP ) cells) had an extended life span, beyond 150 population doublings (PD). In contrast, normal MDF cells failed to proliferate beyond 10 PD. MDF( E6-EGFP ) cells expressed tendon/ligament phenotype-related genes such as Scleraxis (Scx), growth and differentiation factor-5, EphA4, Six-1, and type I collagen. In addition, the expression of periostin was observed. To elucidate the differentiation capacity of MDF( E6-EGFP ) cells in vivo, the cells were transplanted into severe combined immunodeficiency mice. At 4 weeks, MDF( E6-EGFP ) cell transplants had the capacity to generate a PDL-like tissue that expressed periostin, Scx, and type XII collagen and the fibrillar assembly of type I collagen. Our findings suggest that MDF( E6-EGFP ) cells can act as PDL progenitors, and that these cells may be a useful research tool for studying PDL formation and for developing regeneration therapies.

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Section: Discussionmentioning

confidence: 99%

Establishment of immortalized dental follicle cells for generating periodontal ligament in vivo

Yokoi

Saito

Kiyono³

et al. 2006

Cell Tissue Res

120

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“…Immortalized cell lines with distinct phenotypes would circumvent the difficulties. We have already demonstrated that the strain of Trp53 À/À mouse is a useful source for establishing clonal cell lines from various tissues, including the oviduct [21][22][23][24][25], and that the established cell lines express developmental phenotypes [9,[26][27][28]. Oviductal cell lines allow us to reconstruct models in which we can analyze epithelial-mesenchymal interactions.…”

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confidence: 99%

Reconstruction of Oviduct and Demonstration of Epithelial Fate Determination in Mice1

Yamanouchi¹,

Umezu²,

Tomooka³

2010

Biology of Reproduction

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The mouse oviductal epithelium is a simple monolayer until Postnatal Day 7 and subsequently consists of differentiated secretory cells and ciliated cells. In adult oviduct, the two types of epithelial cells are unevenly distributed; ciliated cells are dominant in the ampulla and secretory cells are dominant in the isthmus. Recombinants of enzymatically separated epithelial and mesenchymal tissues of oviducts were grafted under kidney capsule for 4 wk. The recombinants developed structures with a lumen covered with a monolayer of ciliated cells and secretory cells, demonstrating that the recombinant tissues reconstructed oviductal structure. Geographically (ampulla versus isthmus) heterotypic recombinants were prepared from neonatal oviducts at Day 3. The epithelia in reconstructed oviducts took the patterns of cell distribution depending on the origin of the mesenchymal tissues. The results indicate that the mesenchyme geographically has distinct abilities to determine undifferentiated epithelial cells to ciliated cells or secretory cells in the mouse oviduct.

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“…To circumvent problems of small tissue scale and to develop reproducible in vitro models, we established epithelial and mesenchymal clonal cell lines and attempted to investigate the mechanism of how mesenchyme determines the fate of epithelial cells in the oviduct. We have already demonstrated that the p53 −/− mouse is a useful source for establishing clonal cell lines with tissue-specific phenotypes (20,(22)(23)(24)(25) and developmental-stage-specific phenotypes (21,(26)(27)(28)(29).…”

mentioning

confidence: 99%

Follistatin-like-1, a diffusible mesenchymal factor determines the fate of epithelium

Umezu

Yamanouchi

Iida

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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Mesenchyme is generally believed to play critical roles in "secondary induction" during organogenesis. Because of the complexity of tissue interactions in secondary inductions, however, little is known about the precise mechanisms at the cellular and molecular levels. We have demonstrated that, in mouse oviductal development, the mesenchyme determines the fate of undetermined epithelial cells to become secretory or cilial cells. We have established a model for studying secondary induction by establishing clonal epithelial and mesenchymal cell lines from perinatal p53 −/− mouse oviducts. The signal sequence trap method collected candidate molecules secreted from mesenchymal cell lines. Naive epithelial cells exposed to Follistatin-like-1 (Fstl1), one of the candidates, became irreversibly committed to expressing a cilial epithelial marker and differentiated into ciliated cells. We concluded that Fstl1 is one of the mesenchymal factors determining oviductal epithelial cell fate. This is a unique demonstration that the determination of epithelial cell fate is induced by a single diffusible factor.cell fate determination | epithelial-mesenchymal interaction | Follistatinlike-1 | oviduct

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Establishment of uterine cell lines from p53-deficient mice

Cited by 26 publications

References 6 publications

Establishment of immortalized dental follicle cells for generating periodontal ligament in vivo

Establishment of immortalized dental follicle cells for generating periodontal ligament in vivo

Reconstruction of Oviduct and Demonstration of Epithelial Fate Determination in Mice1

Follistatin-like-1, a diffusible mesenchymal factor determines the fate of epithelium

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