Establishment of Two Rat Osteosarcoma Cell Lines (YROS-1 and YROS-2) Induced by Radioactive Phosphorus

Machida, Jiro; Koshino, Tomihisa; Yoshida, Nobuyuki; Hiruma, Toru; Miyagi, Yohei; Aoki, Ichiro; Watanabe, Sara; Kuchino, Yoshiyuki; Misugi, Kazuaki; Nagashima, Yoji

doi:10.1016/s0344-0338(11)80740-5

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Cell Lines and Cell Culture1

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1998

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“…The YROS-1 cell line was established from a radioactive phosphorus ( 32 P)-induced rat osteosarcoma, which possesses osteoblastic characters such as high alkaline phosphate activity and well-developed rough-surfaced endoplasmic reticulum with pericellular deposition of calcified matrix [22]. The YCR cell line derived from a 62-year-old female patient with RCC [23] was used as a target cell line.…”

Section: Cell Lines and Cell Culturementioning

confidence: 99%

Stimulation of Motility of Human Renal Cell Carcinoma by SPARC/Osteonectin/BM-40 Associated with Type IV Collagen

Kato¹,

Sakai²,

M³

et al. 1998

Invasion Metastasis

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SPARC is known to be important in development and tissue remodelling. Here, we examined the effects of SPARC (secreted protein, acidic and rich in cysteine; osteonectin) derived from a rat osteosarcoma cell line on migration of renal cell carcinoma (RCC) by a Boyden chamber assay. YCR RCC cells migrated through type IV collagen-coated filters without stimuli (basal level). SPARC in the lower compartment stimulated chemotactic activity to 120% of the basal level, whereas premixing of YCR with purified SPARC before inoculation reduced their migration to 72% of the basal level. Furthermore, SPARC mixed with type IV collagen more efficiently stimulated their migration in a concentration-dependent manner (up to 170% of the basal level). This suggests that SPARC bound to type IV collagen plays a role in tumor invasion.

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Section: Cell Lines and Cell Culturementioning

confidence: 99%