Establishment of two distinct anti-cardiolipin antibody-producing cell lines from the same individual by Ebstein-Barr virus transformation

A monoclonal IgG anticardiolipin antibody from a patient with the antiphospholipid syndrome is thrombogenic in mice.

“…During the course of our studies, Hasegawa et al reported the generation of two monoclonal IgM ACA by transforming peripheral blood mononuclear cells from a 38-year-old woman with primary APS (47). Of these two mAbs, one displayed LAC activity.…”

The clonal analysis of anticardiolipin antibodies in a single patient with primary antiphospholipid syndrome reveals an extreme antibody heterogeneity

“…In addition, some studies have shown that anti-␤2GP1 antibodies (Abs) are primarily associated with thrombosis in APS [22][23][24] although other reports have involved others Abs as anti-PT 25 or lupus anticoagulant. 26 It appears that most of these difficulties are related to the use of polyclonal Abs (serum or purified immunoglobulin [Ig]) 7,8,10,11 or a few monoclonal IgM 15,[27][28][29] or IgG [30][31][32][33] aPLs originating from different patients. We therefore considered it necessary to concentrate our attention on aPL reactivity of each Ab making up the polyclonal aPL reactivity, breaking down this reactivity at the clonal level in a single patient.…”

Acute cytomegalovirus infection and venous thrombosis: Role of antiphospholipid antibodies

“…A single individual can produce both types of anticardiolipin antibodies. 26 Gharavi et al demonstrated that pathogenic aPL antibodies can be generated by immunizing mice with a CMV peptide. 27 These aPL antibodies have binding characteristics and functional properties similar to those found in the serum of patients with APS.…”