Establishment of Transgene‐Free Porcine Induced Pluripotent Stem Cells
J. Vanessa Conrad,
Jaime A. Neira,
Margaret Rusteika
et al.
Abstract:Although protocols to generate authentic transgene‐free mouse and human induced pluripotent stem cells (iPSCs) are now well established, standard methods for reprogramming porcine somatic cells still suffer from low efficiency and transgene retention. The Basic Protocol describes reprogramming procedures to establish transgene‐free porcine iPSCs (PiPSCs) from porcine fibroblasts. This method uses episomal plasmids encoding POU5F1, SOX2, NANOG, KLF4, SV40LT, c‐MYC, LIN28A, and microRNA‐302/367, combined with an… Show more
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