Establishment of the Mechanism of Cytokinesis in Animal Cells

Rappaport, R.

doi:10.1016/s0074-7696(08)61065-7

Cited by 371 publications

(228 citation statements)

References 107 publications

Supporting

Mentioning

224

Contrasting

Order By: Relevance

“…Furthermore, these structures play crucial roles during cell division; microtubules build up the mitotic spindle that separates the chromosomes, 354 and actin filaments take an active part in the division of the cytoplasm during cytokinesis. 355 Distinct changes in the organization of the cytoskeleton have been observed in cells stimulated with growth factors. EGF caused actin reorganization and membrane ruffling in responsive cells.…”

Section: Role Of Cytoskeletonmentioning

confidence: 99%

Regulation of differentiated properties and proliferation of arterial smooth muscle cells.

et al. 1990

View full text Add to dashboard Cite

Section: Role Of Cytoskeletonmentioning

confidence: 99%

Regulation of differentiated properties and proliferation of arterial smooth muscle cells.

et al. 1990

View full text Add to dashboard Cite

“…In vertebrate cells, centrosome activity is linked to cytokinesis and to the G 1 -to S-phase progression (Rappaport, 1986;Hinchcliffe et al, 2001;Khodjakov and Rieder, 2001). The presence of the centrosome organelle is essential for the viability of vertebrate somatic cells (Doxsey, 2001a).…”

Section: Introductionmentioning

confidence: 99%

Identification of a novel centrosome/microtubule-associated coiled-coil protein involved in cell-cycle progression and spindle organization

et al. 2004

View full text Add to dashboard Cite

Here we describe the identification of a novel vertebratespecific centrosome/spindle pole-associated protein (CSPP) involved in cell-cycle regulation. The protein is predicted to have a tripartite domain structure, where the N-and C-terminal domains are linked through a coiledcoil mid-domain. Experimental analysis of the identified domains revealed that spindle association is dependent on the N-terminal and the coiled-coil mid domain. The expression of CSPP at the mRNA level was detected in all tested cell lines and in testis tissue. Ectopic expression of CSPP in HEK293T cells blocked cell-cycle progression in early G 1 phase and in mitosis in a dose-dependent manner. Interestingly, mitosis-arrested cells contained aberrant spindles and showed impairment of chromosome congression. Inhibition of CSPP gene expression by small interfering RNAs induced cell-cycle arrest/delay in S phase. This phenotype was characterized by elevated levels of cyclin A, decreased levels of cyclin E and hyperphosphorylation of the S-phase checkpoint kinase Chk1. The activation of Chk1 may indicate a replication stress response due to an inappropriate G 1 /S-phase transition. Taken together, we demonstrate that CSPP is associated with centrosomes and microtubules and may play a role in the regulation of G 1 /S-phase progression and spindle assembly.

show abstract

“…For faithful transmission of the genetic material, initiation of cytokinesis must be tightly coupled with the progression of chromosome movement. Experimental data suggest that the mitotic spindle, which drives the separation of chromosomes, is also responsible for determining where and when cytokinesis will occur (Rappaport and Rappaport, 1974;Rappaport, 1986). Two structures of the mitotic spindle, spindle asters (Rappaport, 1961) and the central spindle, have been implicated in induction of cleavage furrows.…”

Section: Introductionmentioning

confidence: 99%

Role of the Midbody Matrix in Cytokinesis: RNAi and Genetic Rescue Analysis of the Mammalian Motor Protein CHO1

Matulienė

Kuriyama

2004

MBoC

View full text Add to dashboard Cite

CHO1 is a kinesin-like motor protein essential for cytokinesis in mammalian cells. To analyze how CHO1 functions, we established RNAi and genetic rescue assays. CHO1-depleted cells reached a late stage of cytokinesis but fused back to form binucleate cells because of the absence of the midbody matrix in the middle of the intercellular bridge. Expression of exogenous CHO1 restored the formation of the midbody matrix and rescued cytokinesis in siRNA-treated cells. By analyzing phenotypes rescued with different constructs, it was shown that both motor and stalk domains function in midbody formation, whereas the tail is essential for completion of cytokinesis after the midbody matrix has formed. During the terminal stage of cytokinesis, different subregions of the tail play distinctive roles in stabilizing the midbody matrix and maintaining an association between the midbody and cell cortex. These results demonstrate that CHO1 consists of functionally differentiated subregions that act in concert to ensure complete cell separation.

show abstract

Establishment of the Mechanism of Cytokinesis in Animal Cells

Cited by 371 publications

References 107 publications

Regulation of differentiated properties and proliferation of arterial smooth muscle cells.

Regulation of differentiated properties and proliferation of arterial smooth muscle cells.

Identification of a novel centrosome/microtubule-associated coiled-coil protein involved in cell-cycle progression and spindle organization

Role of the Midbody Matrix in Cytokinesis: RNAi and Genetic Rescue Analysis of the Mammalian Motor Protein CHO1

Contact Info

Product

Resources

About