Establishment of the cells useful for murine interleukin-18 bioassay by introducing murine interleukin-18 receptor cDNA into human myelomonocytic KG-1 cells

Taniguchi, Mutsuko; Nagaoka, Katsue; Ushio, Shimpei; Nukada, Yoshiyuki; Okura, Takanori; Mori, T.; Yamauchi, Hiroshi; Ohta, Toshio; Ikegami, Hiromasa; Kurimoto, Masashi

doi:10.1016/s0022-1759(98)00098-2

Cited by 13 publications

(8 citation statements)

References 15 publications

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“…In the case of leukemia patients, some of them show elevated level of IL-18 as reported by us. 12 Therefore, it would be interesting to compare the relationship between IL-18R expression in patients with leukemia, especially myeloid leukemia patients, with serum IL-18 levels to determine the possibility of autocrine stimulation.…”

Section: Discussionmentioning

confidence: 99%

“…[9][10][11] Elevated IL-18 was observed in some patients with leukemia, especially those with acute lymphoblastic leukemia (ALL) and chronic myelocytic leukemia (CML). 12 The receptor for human IL-18 was recently identified as an already-reported orphaned receptor, IL-1Rrp (IL-1 receptorrelated protein) by Torigoe et al 13 They purified the IL-18R protein from a Hodgkin's disease cell line, L-428, and determined its amino acid sequence after lectin-and antibody-based affinity chromatography. They clearly showed by cold target inhibition assay and transfectant experiments that IL-1Rrp specifically binds IL-18 but not IL-1␤.…”

Section: Introductionmentioning

confidence: 99%

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Expression and responsiveness of human interleukin-18 receptor (IL-18R) on hematopoietic cell lines

et al. 2000

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression and responsiveness of human interleukin-18 receptor (IL-18R) on hematopoietic cell lines

et al. 2000

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“…It has been reported that malignant proliferation of leukemic cells is supported by a cytokine network surrounding these cells, produced partially by the cells themselves [ 19 ]. Elevated levels of IL-18 were observed in some leukemia patients, especially those with acute lymphoblastic leukemia and CML [ 20 ]. On the other hand, IL-18 receptor expression was reported mostly from CD19+ B cells and some CD8+ T cells [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients

Yalçın

Mutlu²,

Çetin³

et al. 2016

Tjh

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Objective:Interleukin-18 (IL-18) is a cytokine that belongs to the IL-1 superfamily and is secreted by various immune and nonimmune cells. Evidence has shown that IL-18 has both anticancer and procancer effects. The aim of this study was to evaluate the relationship between IL-18 gene polymorphisms and susceptibility to chronic lymphocytic leukemias (CLL) and chronic myelogenous leukemias (CML) in Turkish patients.Materials and Methods:The frequencies of polymorphisms (rs61667799(G/T), rs5744227(C/G), rs5744228(A/G), and rs187238(G/C)) were studied in 20 CLL patients, 30 CML patients, and 30 healthy individuals. The genotyping was performed by polymerase chain reaction and DNA sequencing analysis.Results:Significant associations were detected between the IL-18 rs187238(G/C) polymorphism and chronic leukemia. A higher prevalence of the C allele was found in CML cases with respect to controls. The GC heterozygous and CC homozygous genotypes were associated with risk of CML when compared with controls. However, prevalence of the C allele was not significantly high in CLL cases with respect to controls. There was only a significant difference between the homozygous CC genotype of CLL patients and the control group; thus, it can be concluded that the CC genotype may be associated with the risk of CLL. Based on our data, there were no significant associations between the IL-18 rs61667799(G/T), rs5744227(C/G), or rs5744228(A/G) polymorphisms and CLL or CML.Conclusions:IL-18 gene promoter rs187238(G/C) polymorphism is associated with chronic leukemia in the Turkish population. However, due to the limited number of studied patients, these are preliminary results that show the association between -137G/C polymorphism and patients (CLL and CML). Further large-scale studies combined with haplotype and expression analysis are required to validate the current findings.

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“…These suggest that IL-18 may be produced in excess from lymphoma cells and leukemia cells such as tumor-associated protein in hematological malignancies. Furthermore, in the 7 cases, IL-18 bioactivity was determined by the ability of the cytokine to produce IFN-γ in response to human IL-18 in human myelomonocytic KG-1 cells, as described previously [1, 2, 5, 6]. The results are summarized in table 1.…”

Section: Introductionmentioning

confidence: 99%

Human IL-18 Bioactivity in Hematological Malignancies with Highly Elevated Serum IL-18 Levels

Takubo¹,

Okura²,

Kumura³

et al. 2000

Acta Haematol

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Establishment of the cells useful for murine interleukin-18 bioassay by introducing murine interleukin-18 receptor cDNA into human myelomonocytic KG-1 cells

Cited by 13 publications

References 15 publications

Expression and responsiveness of human interleukin-18 receptor (IL-18R) on hematopoietic cell lines

Expression and responsiveness of human interleukin-18 receptor (IL-18R) on hematopoietic cell lines

The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients

Human IL-18 Bioactivity in Hematological Malignancies with Highly Elevated Serum IL-18 Levels

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