Establishment of temporomandibular joint puncture technique in rats usingin vivomicro-computed tomography (R_mCT^®)

Abstract

The aim of the report was to establish puncture techniques for the temporomandibular joint (TMJ) cavity in rats. The experimental sample comprised 30 male Sprague-Dawley rats. Under general anaesthesia the superior joint cavity of the rat was punctured either laterally (lateral puncture technique (LPT), n 5 11), anteriorly (anterosuperior puncture technique (ASPT), n 5 13) or anteroinferior puncture technique (AIPT), n 5 6) using a 27-gauge needle. After the tip of the needle was confirmed by micro-CT (R-mCT H , Rigaku, Tokyo, Japan) located on the mandibular fossa, 0.05 ml of contrast media was injected under micro-CT fluoroscopic guidance. After confirmation that the joint cavity was filled with contrast media, micro-CT imaging was carried out. The puncture for LPT was accurate in 5 of the 11 animals. The ASPT was accurate in all 13 animals. The AIPT punctured 3 of the 6 animals. Furthermore, the ASPT and AIPT demonstrated improved preservation of the needle; it was harder to detach the needle, which led to greater stability. These results suggest that ASPT assisted by R-mCT H is useful for basic research, including drug discovery and pathogenesis of TMJ diseases.

Kynurenic acid modulates experimentally induced inflammation in the trigeminal ganglion

“…The CFA injection was performed with the antero-superior technique, established by Kameoka et al [ 41 ], into the right upper TMJ. Briefly, the TMJ region was identified by palpation.…”

“…A 27-gauge needle was advanced into the TMJ space along the superomedial border of the zygomatic arch until it reached the condyle. This method of administration to the TMJ has been shown to be very accurate according to arthrographic CT of the joint, using contrast media [ 41 ].…”

Detection and Early Phase Assessment of Radiation-Induced Lung Injury in Mice Using Micro-CT

“…Also, the radiation dose of typical micro-CT scans used for mouse lungs is below 10 to 30 mGy [35], [36], [37], [38], over 50 times less than the values capable of inducing significant lung damage [25]. Therefore, it can thus be repeated in individual animals, i.e., micro-CT enables longitudinal studies.…”

Low‐intensity pulsed ultrasound stimulation for mandibular condyle osteoarthritis lesions in rats

“…The MIA was dissolved in 50 μl of contrast media (Optiray 320, FujiPharma, Fujiyama, Japan) and injected with the lateral puncture technique into the upper compartment of the TMJ using a 27‐gauge 0.5‐inch needle while the rats were under anaesthesia by isoflurane inhalation without surgical assistance. To ensure that MIA was introduced correctly in all rats, we used contrast media instead of saline to confirm that the joint cavity was filled into the upper compartment of the TMJ, using in vivo microcomputed tomography (micro‐CT) postinjection (Kameoka et al., ) (Figure ).…”

“…In vivo micro‐CT (R_mCT2; Rigaku Corporation, Tokyo, Japan) was used as previously described (Kameoka et al., ). The exposure parameters were 3 min, 90 kV and 160 μA.…”