“…VIGS in C. roseus leaf tissue has been pivotal in identifying TIA biosynthesis enzymes and regulatory factors (Carqueijeiro et al, 2015 ; Cruz et al, 2020 ; Liscombe & O'Connor, 2011 ; Yamamoto et al, 2021 ). Increasingly, Agrobacterium infiltration of C. roseus cotyledons, leaf tissue, and flower tissue has been used to overexpress transcription factors and monitor their effects on transcript levels or promoter activity, thus allowing rapid screening of transcription factor function (Carqueijeiro et al, 2021 ; Colinas et al, 2021 ; Colinas & Goossens, 2022 ; Di Fiore et al, 2004 ; Koudounas et al, 2022 ; Kumar et al, 2015 ; Kumar et al, 2018 ; Kumar et al, 2020 ; Mortimer et al, 2020 ; Pan et al, 2019 ; Schweizer et al, 2018 ; Van Moerkercke et al, 2016 ; Yang et al, 2023 ).…”