Establishment of Primary Cell Culture From Ascitic Fluid and Solid Tumor Obtained From Epithelial Ovarian Carcinoma Patients

Kar, Rajarshi; Chawla, Diwesh; Gupta, Bindiya; Mehndiratta, Mohit; Wadhwa, Neelam; Agarwal, Rachna

doi:10.1097/igc.0000000000001087

Cited by 12 publications

(13 citation statements)

References 15 publications

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“…The maintenance of the characteristics of tumor cells in a multicellular organization, to mimic the spheroids observed in the ascitic fluids of ovarian carcinoma patients, is made particularly complicated by the alteration of the cellular response due to the freezing procedure and culture passage [20,21]. The literature data reported the successful creation of several ovarian carcinoma cell lines [20,22,23], although often not taking into account the essential role of spheroids in ovarian cancer progression. It is necessary to bear in mind that established cell lines are an in vitro simplification of the tumor microenvironment and that heterogeneity is observed.…”

Section: Discussionmentioning

confidence: 99%

The Extracellular Matrix Influences Ovarian Carcinoma Cells’ Sensitivity to Cisplatinum: A First Step towards Personalized Medicine

Balduit

Agostinis

Mangogna

et al. 2020

Cancers

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The development of personalized therapies for ovarian carcinoma patients is still hampered by several limitations, mainly the difficulty of predicting patients’ responses to chemotherapy in tumor cells isolated from peritoneal fluids. The main reason for the low predictive power of in vitro assays is related to the modification of the cancer cells’ phenotype induced by the culture conditions, which results in changes to the activation state and drug sensitivity of tumor cells compared to their in vivo properties. We have defined the optimal culture conditions to set up a prognostic test to predict high-grade serous ovarian carcinoma (HGSOC) patients’ responses to platinum chemotherapy. We evaluated the effects of hyaluronic acid (HA) and fibronectin matrices and the contribution of freezing/thawing processes to the cell response to platinum-based treatment, collecting spheroids from the ascitic fluids of 13 patients with stage II or III HGSOC. Our findings indicated that an efficient model used to generate predictive data for in vivo sensitivity to platinum is culturing fresh spheroids on HA, avoiding the use of previously frozen primary tumor cells. The establishment of this easy, reproducible and standardized testing method can significantly contribute to an improvement in therapeutic effectiveness, thus bringing the prospect of personalized therapy closer for ovarian carcinoma patients.

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Section: Discussionmentioning

confidence: 99%

The Extracellular Matrix Influences Ovarian Carcinoma Cells’ Sensitivity to Cisplatinum: A First Step towards Personalized Medicine

Balduit

Agostinis

Mangogna

et al. 2020

Cancers

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“…However, the majority of patients eventually become resistant to chemotherapeutic drugs and this causes the prognosis of these patients to be unsatisfactory ( 20 ). Previous studies for ovarian cancer were primarily based on cells generated from primary lesions ( 21 , 22 ), and few of these studies focused on the omentum ( 23 ). It has been suggested that metastatic cancer is a markedly heterogeneous disease at the genetic, transcriptomic and microenvironment levels ( 24 ).…”

Section: Discussionmentioning

confidence: 99%

Real‑time assessment of platinum sensitivity of primary culture from a patient with ovarian cancer with extensive metastasis and the platinum sensitivity enhancing effect by metformin

Liu

Yan

et al. 2018

Oncol Lett

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The aim of the present study was to perform a rapid evaluation of the efficiency of commonly used platinum-based chemotherapy regimens for patients with ovarian cancer with extensive metastases using an in vitro method combined with culturing primary cells and real-time monitoring, and to further explore the enhanced effect of metformin on susceptibility of ovarian cancer cells to platinum-based chemotherapy. The primary omental metastatic (OM) cells were isolated from the omentum metastasis of a surgical patient with stage IIIc ovarian carcinoma. Drug sensitivity was evaluated using the xCELLigence system, and screening of the most effective platinum chemotherapy was performed through analysis of cell susceptibility to cisplatin, carboplatin, nedaplatin and paclitaxel or docetaxel alone or in combination. At the same time, this system was used to determine whether metformin was able to increase the sensitivity of cancer cells to platinum chemotherapy. The results revealed that nedaplatin exhibited the most marked cytotoxic effect on the OM cells, followed by those of carboplatin and cisplatin. The addition of docetaxel enhanced the cytotoxic effect, and the combination of platinum and paclitaxel also enhanced the effect. Metformin rapidly increased the sensitivity of cells to platinum-based chemotherapy, and this effect was dose-dependent. The sensitivity of OM cells to different platinum-based regimens was varied. The effect of metformin on chemotherapeutic sensitization of cancer cells is clear in vitro, and the real-time cell analyzer assay has the potential to assist in determining individualized drug regimens for patients with metastatic ovarian cancer.

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“…Qiu X et.al разработали микрофлюидное устройство, позволяющее проводить более мягкую механическую дезагрегацию клеток, используя сеть каналов и «гидродинамические скальпели» [21]. В исследовании Kar et.al успешно получали первичные клеточные линии рака яичника как с помощью механической диссоциации ткани, так и ферментативно с помощью Dispase II [22].…”

Section: механическая диссоциацияunclassified

Primary tumor cell cultures: сurrent methods of obtaining and subcultivation

Mezhevova

Ситковская

2020

jour

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Over the past decades, transplantable cell lines have been an affordable model for studying the biology and effect of chemotherapeutic drugs on tumors. However, numerous studies have shown that these cell lines are not heterogeneous enough and cannot reflect the drug resistance of tumors that occurs in some patients. Primary cell line cultures isolated from solid tumors have become widespread in personalized cancer therapy. This review discusses the basic methods for the preparation and cultivation of primary cell lines. A brief description is given of the methods for the disaggregation of tumor material using enzymatic, chemical and mechanical dissociation. The systems of cultivation of primary cell cultures. The selection of an appropriate dissociation method and cultivation is important to preserve the benefits of primary culture in preclinical studies.

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Establishment of Primary Cell Culture From Ascitic Fluid and Solid Tumor Obtained From Epithelial Ovarian Carcinoma Patients

Cited by 12 publications

References 15 publications

The Extracellular Matrix Influences Ovarian Carcinoma Cells’ Sensitivity to Cisplatinum: A First Step towards Personalized Medicine

The Extracellular Matrix Influences Ovarian Carcinoma Cells’ Sensitivity to Cisplatinum: A First Step towards Personalized Medicine

Real‑time assessment of platinum sensitivity of primary culture from a patient with ovarian cancer with extensive metastasis and the platinum sensitivity enhancing effect by metformin

Primary tumor cell cultures: сurrent methods of obtaining and subcultivation

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