Establishment of permanent cell lines purified from human mesothelioma: morphological aspects, new marker expression and karyotypic analysis

Abstract

This study reports the establishment of three major subtypes of human mesothelioma cells in tissue culture, i.e. the epithelioid, sarcomatoid and biphasic forms, and compares their phenotypic and biological characteristics. Primary cells isolated from biopsies or pleural exudates were subcultured for over 50 passages. We evaluated immunoreactivity using various mesothelial markers related to histological patterns of these cell lines. For epithelioid cells, calretinin and cytokeratin were found to be useful and easily interpretable markers as for control mesothelial cells. The biphasic form was only partially positive and the sarcomatoid type negative. Vimentin was expressed by all cell lines. BerEP4, a specific marker for adenocarcinoma, was negative. Interestingly, while the macrophage marker CD14 was negative, immunoreactivity for a mature macrophage marker (CD68) was expressed by all cell types, suggesting that this marker might constitute an additional tool useful in the differential diagnosis of mesothelioma. At the ultrastructural level, a cell surface rich in microvilli confirmed their mesothelial origin. PCR analysis revealed that none of the cell lines contained SV40 DNA. Karyotypic analyses showed more complex abnormalities in the epithelioid subtype than in the sarcomatoid form. These cell lines may be useful in the study of cellular, molecular and genetic aspects of the disease.

Phenotypes and Karyotypes of Human Malignant Mesothelioma Cell Lines

“…The morphological and growth characteristics of the six cell lines reported here were consistent with those previously documented for mesothelioma cell lines [9]–[11]. One cell line (PF1038) underwent morphological change during passaging possibly as a result of selective adaptation to artificial culture conditions [17].…”

“…Although characterised mesothelioma cell lines exist [9]–[11], few are available in tumour banks such as American Type Culture Collection (ATCC), European Collection of Animal Cell Cultures (ECACC) and Cell Bank Australia. In the era of next generation sequencing, a repository of molecularly and biologically characterised and clinicopathologically annotated early passage cell lines represent a valuable resource for mesothelioma research.…”

An orthotopic implantation mouse model of human malignant pleural mesothelioma for in vivo photon counting analysis and evaluation of the effect of S‐1 therapy

“…The phenotypic characteristics of the 3 HMPM cell lines in our study were comparable to those of the original parent HMPM cells isolated from the patients and also other well‐known HMPM cell lines (NCI‐H28, NCI‐H226 and MSTO‐211H), e.g. , in terms of the positive expression of vimentin, hyaluronic acid, TPA, IL‐6 and VEGF, reported previously 6, 7, 10, 14, 38, 39. Recently, ERC/mesothelin has been reported as a new clinical marker for HMPM 31, 32, 40.…”

Variation in Drug Sensitivity of Malignant Mesothelioma Cell Lines with Substantial Effects of Selenite and Bortezomib, Highlights Need for Individualized Therapy

“…MM cell lines: STAV-AB, STAV-FCS and ZL-34 cells were kindly provided by Julius Klominek [34], [35]. DM-3 and JL-1 cells were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) [36]. M-14-K cells were kindly provided by K. Linnainmaa [37] and Jurkat cells were obtained from the American Type Culture Collection (ATCC) [38].…”