Establishment of Ovarian Cancer Cell Lines

Abstract

Human tumor cell lines have provided valuable model systems to study a wide variety of tumor characteristics including the cell biology, genetics, and chemosensitivity profiles of disease. A large number of ovarian cancer cell lines have now been established and are in widespread use Table 1) (1-15). Many of these have been selected to reflect specific situations, e.g., pre- and postchemotherapy models or different histo- logical subtypes. Table 1 Properties of Established Ovarian Carcinoma Cell Lines Prior Cell Line Histology Source Treatment Ref. PE01 P.D. Serous adenoca Ascites P/FU/CHL 1 PE04 P.D. Serous adenoca Ascites P/FU/CHL 1 PE06 P.D. Serous adenoca Ascites P/FU/CHL 2 PEA1 P.D. Adenoca Pleural None 2 PEA2 P.D. Adenoca Ascites P/Pred 2 PE016 P.D. Serous adenoca Ascites Radioth 2 PE014 W.D.Serous adenoca Ascites None 2 T014 W.D.Serous adenoca Sol. Met None 2 PE023 W.D.Serous adenoca Ascites P/CHL 2 SKOV-3 Adenoca Ascites T 3 SW626 Adenoca - - 3 OVCAR-2 Adenoca Ascites P/Cy 4 OVCAR-3 P.D. papillary adenoca Ascites P/Cy/Adr 5 OVCAR-4 Adenoca Ascites P/Cy/Adr 6 OVCAR-5 Adenoca Ascites None 7 OAW 28 Adenoca Ascites P / Mel 8 OAW 42 Serous adenoca Ascites P 8 41M Adenoca Ascites None 9 59M Endometr adenoca Ascites None 8 CH1 Papillary adenoca Ascites P/ JM8 8 138D Serous adenoca Ascites Carb 9 180D Adenoca Ascites P 9 200D Serous adenoca Solid None 9 253D Serous adenoca Ascites Cy/MPA 9 HOC-1 W.D. Serous adenoca Ascites None 10 HOC-7 W.D. Serous adenoca Ascites None 10 CAOV-3 Adenoca Tumour Cy/Adr/FU 10 COLO 110 Serous adenoca Sol. Met None 11 COLO 316 Serous adenoca Pleural None 11 COLO 319 Serous adenoca Ascites None 11 COLO 330 Serous adenoca Ascites Mel/Radiother 11 IGROV1 Adenoca Primary None 12 HTOA W.D. serous adenoca Primary None 13 OV-1063 Papillary adenoca Ascites Cy/Adr/P/HMM 14 DO-s W.D. mucinous adenoca Ascites - 15 P.D. = Poorly differentiated; W.D. = Well differentiated; adenoca = adenocarcinoma; pleural = pleural effusion; Sol.met. = solid metastasis; P = cisplatin; FU = 5-fluorouracil; CHL = chlorambucil; Pred = prednimustine; Radioth = radiotherapy; T = thiotepa; Cy = cyclophosphamide; Adr = adriamycin; Mel = melphalan; Carb = carboplatin; MPA = medroxyprogesterone actetate; HMM = examethylmelamine.

Modeling Platinum Sensitive and Resistant High-Grade Serous Ovarian Cancer: Development and Applications of Experimental Systems

“…Isolation and primary culturing of tumor cells from ascites have become more widespread and several different methods have been established to achieve this aim (5–8). A widely used protocol for the propagation of EOC tumor cells was developed by Langdon et al (6, 9). Freshly drained ascites fluid, mixed with heparin to prevent cell aggregation, is pelleted by centrifugation, resuspended in PBS, and subjected to gradient centrifugation using histopaque or Ficoll-hypaque to remove any contaminating erythrocytes.…”

“…Freshly drained ascites fluid, mixed with heparin to prevent cell aggregation, is pelleted by centrifugation, resuspended in PBS, and subjected to gradient centrifugation using histopaque or Ficoll-hypaque to remove any contaminating erythrocytes. The resulting interface layer is washed in PBS prior to culturing in appropriate tissue culture media, monitored carefully for fibroblast or mesothelial cell contamination, with the EOC tumor cells sub-cultured upon confluency (6, 9, 10). Alternatively, Shepherd et al mix ascites 1:1 with M199/MCDB105 growth medium and monitor EOC tumor cell growth in culture, relying on EOC cells adhering to the plastic and contaminating erythrocytes being removed in the first set of media changes approximately 4 days after initial seeding (7).…”

“…Proliferation of fibroblasts will cease after a number of passages; however, if not contained from initial culturing, fibroblasts can outgrow the tumor cells and take over the culture. Selective trypsinization with low concentrations of trypsin can be used to remove contaminating fibroblasts or mesothelial cells from primary cultures, as they tend to detach more rapidly from plastic than tumor cells (6). However, this may need to be repeated several times to maintain a fibroblast-free culture.…”

“…Langdon et al have advocated mechanical dissociation of tumor fragments using crossed scalpels, with cell suspensions filtered through sterile gauze to remove any remaining cell clumps before placing in culture (6, 10). The laboratories of Nachtigal and Mes-Masson have employed both mechanical disruption of tumor tissue using cell scrapers and enzymatic disruption of tumor using collagenase and concluded that mechanical dissociation was the more efficient method for their purposes (13, 14).…”

Mixed modality treatment planning of accelerated partial breast irradiation: to improve complex dosimetry cases

“…At the Institut Gustave Roussy, we recently reported the early results of a 3D-conformal APBI trial [5] in which treatment planning was performed according to the technique designed by Taghian and colleagues, consisting of 2 mini-tangents and an "en face" electron field contributing around 20% of the total dose (8 Gy) [4,6]. The design of this phase II dose escalation trial was to deliver a total dose of 40 Gy in 10 fractions over 5 days (40 Gy step) and 44 Gy in 10 fractions over 5 days (44Gy step).…”