Establishment of orally-administered Lactobacillus gasseri SBT2055SR in the gastrointestinal tract of humans and its influence on intestinal microflora and metabolism

Fujiwara, Shigeru; Seto, Yasuyuki; Kimura, Akira; Hashiba, Honoo

doi:10.1046/j.1365-2672.2001.01251.x

Cited by 101 publications

(87 citation statements)

References 27 publications

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“…However, many of the effects obtained from viable cells of probiotics are also obtained from populations of dead cells (Adams, 2010) such as induction of B‐cell activation, induction of TNF‐α production, and augmentation of IgA production. In the human gastrointestinal tract, LG2055 establishes and alters the composition and metabolism of the intestinal microflora as well as the physical characteristics of the feces (Fujiwara et al ., 2001). Dead LG2055 has also been shown to provide some health benefits in both mice and humans, and although LG2055 was not shown to be harmful, it was unable to colonize the intestines of C. elegans .…”

Section: Discussionmentioning

confidence: 99%

Effects and mechanisms of prolongevity induced by Lactobacillus gasseri SBT2055 in Caenorhabditis elegans

et al. 2015

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SummaryLactic‐acid bacteria are widely recognized beneficial host associated groups of the microbiota of humans and animals. Some lactic‐acid bacteria have the ability to extend the lifespan of the model animals. The mechanisms behind the probiotic effects of bacteria are not entirely understood. Recently, we reported the benefit effects of Lactobacillus gasseri SBT2055 (LG2055) on animal and human health, such as preventing influenza A virus, and augmentation of IgA production. Therefore, it was preconceived that LG2055 has the beneficial effects on longevity and/or aging. We examined the effects of LG2055 on lifespan and aging of Caenorhabditis elegans and analyzed the mechanism of prolongevity. Our results demonstrated that LG2055 has the beneficial effects on longevity and anti‐aging of C. elegans. Feeding with LG2055 upregulated the expression of the skn‐1 gene and the target genes of SKN‐1, encoding the antioxidant proteins enhancing antioxidant defense responses. We found that feeding with LG2055 directly activated SKN‐1 activity via p38 MAPK pathway signaling. The oxidative stress response is elicited by mitochondrial dysfunction in aging, and we examined the influence of LG2055 feeding on the membrane potential of mitochondria. Here, the amounts of mitochondria were significantly increased by LG2055 feeding in comparison with the control. Our result suggests that feeding with LG2055 is effective to the extend lifespan in C. elegans by a strengthening of the resistance to oxidative stress and by stimulating the innate immune response signaling including p38MAPK signaling pathway and others.

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Section: Discussionmentioning

confidence: 99%

Effects and mechanisms of prolongevity induced by Lactobacillus gasseri SBT2055 in Caenorhabditis elegans

et al. 2015

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“…If the number of enterococci recovered had been decreased, greater Lactobacillus strain diversity may have been revealed. Rif r marking to recover introduced Lactobacillus strains from the indigenous GIT microbiota has been successfully used in chickens (23,43), ducks (16), pigs (22,39), mice (19,41), and humans (20,45), so a Rif r background was unexpected in this study. Rif MIC levels within several Lactobacillus and Pediococcus species have been reported to range between Յ0.016 and 4 g ml Ϫ1 (12,13).…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus Strain Ecology and Persistence within Broiler Chickens Fed Different Diets: Identification of Persistent Strains

Stephenson

Moore

Allison

2010

Appl Environ Microbiol

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Lactobacilli are autochthonous residents in the chicken gastrointestinal tract, where they may potentially be used as probiotics, competitive exclusion agents, or delivery vehicles. The aim of this study was to use an in vivo model to investigate the effect of diet and competing lactic acid bacteria on the colonization of inoculated Lactobacillus strains, with the goal of identifying strains which can consistently colonize or persist for an extended period of several weeks. Chicken-derived Lactobacillus strains were genetically marked with rifampin resistance and administered on day 0 to chickens fed either a normal commercial diet or a specially formulated high-protein diet. Chickens fed the high-protein diet were also coinoculated with two different mixes of additional lactic acid bacteria. Enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) was used to identify rifampin-resistant isolates recovered from chickens. Three strains, belonging to the species Lactobacillus agilis, Lactobacillus crispatus, and Lactobacillus vaginalis, were commonly reisolated from the chickens on both diets at days 21 and 42. The ability of these strains to persist was confirmed in a second chicken trial. All three strains persisted throughout the production period in the chickens fed a commercial diet, while only the L. agilis and L. vaginalis strains persisted in the chickens fed the high-protein diet. In both in vivo trials, competing lactic acid bacteria modified representation of the strains recovered, with all three stains capable of competing in the presence of one or both mixes of coinoculated strains. The in vivo model successfully identified three persistent strains that will be characterized further.The ecology of the chicken gastrointestinal tract (GIT) has been studied in depth using both culture-dependent (5,7,21,40) and -independent methods (2,3,7,33,54). These studies have revealed that lactobacilli are autochthonous residents in chickens, where they predominate in the proximal GIT and are present but less abundant within the distal GIT (52). The most commonly identified Lactobacillus species are Lactobacillus crispatus, Lactobacillus reuteri, and Lactobacillus salivarius (1,7,15,26,28). A detailed understanding of the relationship between these bacteria and their host under different dietary and environmental conditions will facilitate the development of lactobacilli for various applications directed toward increasing broiler production efficiency and improving chicken health.Since the withdrawal of antimicrobial growth promoters from chicken feed in Europe, the incidence of necrotic enteritis (NE) has increased (9, 51). Consequently, there is a need to develop alternative methods for controlling the causative agent of NE, Clostridium perfringens, in the chicken GIT. Lactobacilli are excellent candidates for alternative control methods due to their autochthonous nature and dominance of the upper GIT microbiota, particularly within the small intestine where NE occurs. Their potential utility ...

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“…In human tests, inoculation with Lactobacillus gasseri SBT2055 (dose: 10 11 cfu/day) resulted in an alteration of intestinal microflora communities; Lactobacillus rhamnosus GG (dose: 10 10 cfu/day) colonized the colonic mucosae, and five Lactobacillus strains (dose: 10 8 cfu/day) reached intestine alive after oral administration (Johansson et al, 1993, Alander et al, 1999, Fujiwara et al, 2001. The nature of probiotic strains, of human hosts, and of carrier food all influence the dose necessary to confer health benefits to the host.…”

Section: Strainmentioning

confidence: 99%

Selection of Lactic Acid Bacteria as Starter Cultures for Fermented Meat Products

Tsuda

Matsumoto

Ishimi

2012

FSTR

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The purpose of this study was to select a lactic acid bacterial strain for use as a starter culture for producing fermented meat products and for evaluating the survival ability in the gastrointestinal tract. Five strains of lactic acid bacteria were isolated from funazushi, a traditional fermented fish product in Japan, and their bile and simulated digestive juice tolerances were evaluated to screen their survival in gastrointestinal tracts. Growth in a ground meat patty was evaluated by counting viable cells and measuring pH. Lactobacillus plantarum JAB2001 was capable of growth on 10 % NaCl and exhibited tolerance to bile acids and simulated gastrointestinal juices. After incubation with this strain at 25℃ for 48 h a ground beef patty showed pH values of about 4.8 and lactic acid bacterial counts at the 10 9 level per gram of patty.Coliform bacteria and Staphylococcus aureus were unable to proliferate in the meat patty with strain JAB 2001, which is thus suitable for use as a starter culture of fermented meat products.

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Establishment of orally-administered Lactobacillus gasseri SBT2055SR in the gastrointestinal tract of humans and its influence on intestinal microflora and metabolism

Cited by 101 publications

References 27 publications

Effects and mechanisms of prolongevity induced by Lactobacillus gasseri SBT2055 in Caenorhabditis elegans

Effects and mechanisms of prolongevity induced by Lactobacillus gasseri SBT2055 in Caenorhabditis elegans

Lactobacillus Strain Ecology and Persistence within Broiler Chickens Fed Different Diets: Identification of Persistent Strains

Selection of Lactic Acid Bacteria as Starter Cultures for Fermented Meat Products

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