Establishment of mouse gingival junctional epithelial cell line using a bioengineered tooth system

Seki, Tamotsu; Aizawa, Ryo; Tanaka, Junichi; Yajima-Himuro, Sara; Kato, Mitsuhiro; Tanaka, Keisuke; Mishima, Kenji; Yamamoto, Masahide

doi:10.1016/j.bbrc.2018.02.047

Cited by 8 publications

(6 citation statements)

References 21 publications

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“…We expect that these other proteins were derived from contaminated OE, as the filter papers used to collect iSG saliva were directly attached to the oral mucosa. The protein profile of iSG-derived saliva was compared with the gene expression profile of OE 51 . As we expected, 75% other proteins besides saliva proteins, such as keratins and ribosomal proteins, were expressed in the OE (Supplementary Data 6 ).…”

Section: Resultsmentioning

confidence: 99%

Generation of orthotopically functional salivary gland from embryonic stem cells

et al. 2018

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Organoids generated from pluripotent stem cells are used in the development of organ replacement regenerative therapy by recapitulating the process of organogenesis. These processes are strictly regulated by morphogen signalling and transcriptional networks. However, the precise transcription factors involved in the organogenesis of exocrine glands, including salivary glands, remain unknown. Here, we identify a specific combination of two transcription factors (Sox9 and Foxc1) responsible for the differentiation of mouse embryonic stem cell-derived oral ectoderm into the salivary gland rudiment in an organoid culture system. Following orthotopic transplantation into mice whose salivary glands had been removed, the induced salivary gland rudiment not only showed a similar morphology and gene expression profile to those of the embryonic salivary gland rudiment of normal mice but also exhibited characteristics of mature salivary glands, including saliva secretion. This study suggests that exocrine glands can be induced from pluripotent stem cells for organ replacement regenerative therapy.

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Section: Resultsmentioning

confidence: 99%

Generation of orthotopically functional salivary gland from embryonic stem cells

et al. 2018

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“…The tooth germ transplantation method was performed as previously described 48 . Briefly, molar tooth germs were collected from the mandibles of C57BL/6-Tg (CAG-EGFP) mice on E14.…”

Section: Methodsmentioning

confidence: 99%

Visualization of junctional epithelial cell replacement by oral gingival epithelial cells over a life time and after gingivectomy

Kato

Tanaka

Aizawa

et al. 2019

Sci Rep

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Junctional epithelium (JE), which is derived from odontogenic epithelial cells immediately after eruption, is believed to be gradually replaced by oral gingival epithelium (OGE) over a lifetime. However, the detailed process of replacement remains unclear. The aim of the present study was to clarify the process of JE replacement by OGE cells using a green fluorescent protein (GFP)–positive tooth germ transplantation method. GFP-positive JE was partly replaced by OGE cells and completely replaced on day 200 after transplantation, whereas there was no difference in the expression of integrin β4 (Itgb4) and laminin 5 (Lama5) between JE before and after replacement by OGE cells. Next, GFP-positive JE was partially resected. On day 14 after resection, the regenerated JE consisted of GFP-negative cells and also expressed both Itgb4 and Lama5. In addition, the gene expression profile of JE derived from odontogenic epithelium before gingivectomy was partly different from that of JE derived from OGE after gingivectomy. These results suggest that JE derived from the odontogenic epithelium is gradually replaced by OGE cells over time and JE derived from the odontogenic epithelium might have specific characteristics different to those of JE derived from OGE.

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“…However, by tagging mouse junctional epithelial cells with green fluorescent protein, we were able to isolate and establish 2 immortalized lines-designated JE-1 and JE-2-with the desired properties. 189 JE-1 and JE-2 cells possess some of the signature characteristics of junctional epithelial cells, making them potentially quite useful in future work on the protective state of the periodontal tissue. In fact, our preliminary work shows that JE-1 cells express the genes Cxcl10 and Slc7a11 at significantly higher levels than normal oral epithelial cells (data not shown).…”

Section: Summary and Per S Pec Tive Smentioning

confidence: 99%

“…Unfortunately, no cell lines with junctional epithelial properties were available until recently, which has thus far limited sophisticated in vitro analyses of the mechanisms used to maintain the protective state. However, by tagging mouse junctional epithelial cells with green fluorescent protein, we were able to isolate and establish 2 immortalized lines— designated JE‐1 and JE‐2—with the desired properties 189 . JE‐1 and JE‐2 cells possess some of the signature characteristics of junctional epithelial cells, making them potentially quite useful in future work on the protective state of the periodontal tissue.…”

Section: Summary and Perspectivesmentioning

confidence: 99%

Maintaining a protective state for human periodontal tissue

Yamamoto

Aizawa

2021

Periodontology 2000

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In the 20th century, dentists largely classified periodontal diseases according to patient age at onset, rate of progression, and pathology. Accordingly, the main concepts of periodontitis were "adult," "early-onset," and "refractory"; however, these names did not correspond to the specific bacterial species identified, in such cases, using bacteriological approaches. Therefore, in 1999, the American Academy of Periodontology reorganized this tripartite classification into 2 broad groups: severe, rapid destruction of periodontal tissue not attributable to systemic diseases; and periodontitis as a manifestation of systemic diseases. 1 The former group is considered to be "aggressive periodontitis" (class III in the American Academy of Periodontology Periodontal Disease Classification System), while the latter can be grouped under periodontitis associated with hematological or genetic disorders (classes IVa and IVb in the American Academy of Periodontology Periodontal Disease Classification System), often presenting with comorbid immune (neutrophil) dysfunction. 1,2 However, researchers could not identify specific bacteria or pathologies unique to any of these classes. Subsequently, the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions led to a series of changes in periodontal disease taxonomy, transforming classification into a stage-and grade-based system. 3 | Periodontitis as a multifactorial diseaseClinical manifestations of incipient periodontitis vary considerably across individuals, depending on where the tissue damage originates and how it progresses; thus, it is impossible to explain the symptomatology in terms of bacterial infection alone. We now know that destruction of the periodontal tissue does not progress continuously and linearly, but is rather a result of repeated switches between dormant and active stages of resident bacteria. 4,5 Simplistically describing periodontitis as an infection with certain bacteria cannot completely explain the diverse patterns observed. The fact that periodontitis is a multifactorial disease lies at the heart of this complexity. The tissue damage is thought to be driven by a variety of factors, 6,7 which have started to be illuminated in recent years, and these risk factors have now been conceptually organized into 3 main groups: bacterial, host, and environmental. A host factor can include an underlying metabolic disease that affects the whole body, while smoking is an archetypal environmental factor. Changes in the biological properties of host cells can have an enormous influence on defense mechanisms in the periodontal tissue. In vitro analyses can be used to chronicle the detailed changes in immunocytes and resident cell populations; however, physiological samples are more useful for investigating systemic shifts in host defense mechanisms. Data from analyses of the gingival crevicular fluid have provided intriguing insights into how the protective state evolves and changes as humans grow and age. For example, by comp...

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Establishment of mouse gingival junctional epithelial cell line using a bioengineered tooth system

Cited by 8 publications

References 21 publications

Generation of orthotopically functional salivary gland from embryonic stem cells

Generation of orthotopically functional salivary gland from embryonic stem cells

Visualization of junctional epithelial cell replacement by oral gingival epithelial cells over a life time and after gingivectomy

Maintaining a protective state for human periodontal tissue

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