Establishment of molecular markers and linkage groups in two F2 populations of Upland cotton

Shappley, Z. W.; Jenkins, Jonnie N.; Watson, C. E.; Kahler, A. L.; Meredith, W. R.

doi:10.1007/s001220050211

Cited by 9 publications

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“…Since Reinisch et al. (1994) reported the first molecular map of cotton, afterwards, many linkage maps of cotton have been reported including intraspecific crosses between upland cottons and interspecific crosses (Shappley et al. 1996, 1998, Ulloa and Meredith 2000, Ulloa et al.…”

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“…Because of its economical importance, plant breeders and geneticists have paid much attention to the genetic study on cotton. Since Reinisch et al (1994) reported the first molecular map of cotton, afterwards, many linkage maps of cotton have been reported including intraspecific crosses between upland cottons and interspecific crosses (Shappley et al 1996, Ulloa and Meredith 2000, Ulloa et al 2002, Zhang et al 2002, Lacape et al 2003, Rong et al 2004). However, to date, the research progress in kenaf obviously drops behind cotton, with almost no information available on segregation analysis of genes or molecular markers in a cross population.…”

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Genetic linkage map construction for kenaf using SRAP, ISSR and RAPD markers

Chen

Wei

et al. 2011

Plant Breeding

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Kenaf (Hibiscus cannabinus L.) is one of the most economically important crops for fibre production. Genetic maps have been developed in almost all the agricultural crops. However, genetic information for kenaf, especially, at the molecular level is limited. To better understand the genetic basis of kenaf for the improvement of production and to lay the foundation for molecular breeding efforts, a primary genetic linkage map was constructed using sequence-related amplified polymorphism (SRAP), inter-simple sequence repeat (ISSR) and randomly amplified polymorphic DNA (RAPD). Cultivar ÔAlian kenafÕ and ÔFuhong 992Õ were used as parents to construct an F 2 population consisting of 180 plants. We selected 494 SRAP, 60 ISSR, 120 RAPD and 300 two-primer RAPD mixture primers that amplified 396 polymorphic loci in total. At a logarithm of the odds (LOD) score threshold of 5.0 and at a maximum map distance of 25 cM, these 396 loci were used to construct the genetic linkage map with MAP-MAKER/EXP 3.0, a total of 307 loci were grouped into 26 linkage groups that spanned a total map length of around 4924.8 cM with a mean density of 16.04 cM per locus. These markers were distributed randomly in all linkage groups without any clustering. The construction of the kenaf genetic linkage map will be useful for further genetic studies including mapping both qualitative and quantitative traits, marker-assisted selection program, and comparative genomics analysis.

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Genetic linkage map construction for kenaf using SRAP, ISSR and RAPD markers

Chen

Wei

et al. 2011

Plant Breeding

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“…Restriction fragment length polymorphisms (RFLPs) have been applied to cotton species to study genetic diversity, population genetics, evolutionary history, and genome mapping (Shappley et al, 1996; Wang et al, 1995; Yu et al, 1997). For our purposes, this may not be a viable approach because the level of polymorphism in cotton is quite low compared with other plant taxa (Brubaker and Wendel, 2000).…”

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Estimation of Genetic Divergence among Elite Cotton Cultivars–Genotypes by DNA Fingerprinting Technology

2002

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to CLCD (Ali, 1999). Among those, a diverse genetic base of the cultivars grown in Pakistan was suggested Epidemics of cotton leaf curl virus disease (CLCD) was the compelas an important element in controlling the disease (Zhu ling factor in the decision to devise new strategies for cotton breeding programs of Pakistan. The genetic similarity among the elite cotton et al., 2000); a narrow genetic base may predispose the (Gossypium spp.) cultivars released before the advent of CLCD epi-crop to an epidemic (Holley and Goodman, 1989). demics was in the range of 81.5 to 93.41%. New cultivars were devel-Restriction fragment length polymorphisms (RFLPs) oped by crossing the exotic resistant germplasm (LRA-5166, CP-15/2, have been applied to cotton species to study genetic and Cedix) with adapted varieties highly susceptible to CLCD. A diversity, population genetics, evolutionary history, and study was designed to assess the genetic relatedness or diversity among genome mapping (Shappley et al., 1996; Wang et al., the newly released, extremely resistant and resistant cultivars. After 1995; Yu et al., 1997). For our purposes, this may not screening 27 cotton genotypes by different diagnostic methods such be a viable approach because the level of polymorphism as field evaluation, whitefly-transmission studies, grafting, dot-blotin cotton is quite low compared with other plant taxa hybridization, and multiplex PCR using conserved primers sequences, (Brubaker and Wendel, 2000). Moreover, the analysis 20 extremely resistant and resistant cultivars were selected for a random amplified polymorphic DNA (RAPD) analysis. The genetic simi-The plant material used in the study consisted of 27 cotton cultivars, varieties, or genotypes. These cultivars or varieties

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“…Sequential deletions of the promoter gradually decreased the expression of the GUS gene. Shappley et al (1996) determined the inheritance of restriction fragment length polymorphisms and their linkage relationship in two Upland cotton F 2 populations. The two crosses characterized for restriction fragment length polymorphisms were HyPerformer HS 46 x MAR-CA-BUCAG8US-1-88 (MAR) and HyPerformer HS 46 x PEE DEE 5363 (PD5363).…”

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History of USDA-ARS Cotton Host Plant Resistance and Breeding Research at Mississippi State, MS

McCarty¹,

Jenkins²,

Saha³

et al. 2021

JCS

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Cotton host plant resistance research was initiated with the establishment of the Boll Weevil Research Laboratory in 1960. Laboratory objectives were to conduct research and develop technology that ultimately could be used to eradicate the boll weevil. Early research concentrated on developing techniques and screening germplasm for resistance. A full-scale boll weevil eradication trial began in southern Virginia and eastern North Carolina in 1978 and after initial success the USDA Animal and Plant Health Inspection Service established an eradication program. This led the host plant resistance program to broaden its research into other pests of cotton. During the 1980s, research continued to focus on tarnished plant bug, tobacco budworm, expanding the genetic diversity of cotton, basic genetic and cotton breeding studies. With the development of field infestation techniques for the tobacco budworm, in the 1990s the research team conducted the first field test of Bacillus thuringiensis (Bt) transgenic cotton for resistance. During this time, root-knot nematode research expanded. Cotton fruiting efficiency and distribution of harvestable bolls and the concept of plant mapping were developed. During the 2000s, research expanded with the use of chromosome substitution lines for the introgression of new alleles into Upland cotton. Nematode research remained active during this time. To date, the research program has developed and released more than 800 germplasm lines and four random-mating populations. Scientists in the program have trained more than 60 graduate students and countless others have been mentored. The full impact of the research team will only be revealed with time.

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Establishment of molecular markers and linkage groups in two F2 populations of Upland cotton

Cited by 9 publications

References 0 publications

Genetic linkage map construction for kenaf using SRAP, ISSR and RAPD markers

Genetic linkage map construction for kenaf using SRAP, ISSR and RAPD markers

Estimation of Genetic Divergence among Elite Cotton Cultivars–Genotypes by DNA Fingerprinting Technology

History of USDA-ARS Cotton Host Plant Resistance and Breeding Research at Mississippi State, MS

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