Establishment of Male and Female Eucommia Fingerprints by UPLC Combined with OPLS‐DA Model and Its Application

Abstract

Eucommia ulmoides Oliver is a dioecious plant, which plays an important role in traditional Chinese medicine. However, there has not yet been any research on male and female E. ulmoides. The UPLC fingerprints and OPLS-DA approach were able to quickly and easily identify and quantify E. ulmoides and differentiate between the male and female fingerprints. In this study, we optimized the UPLC conditions and analyzed them to investigate fingerprints of twenty-four extracts of Eucommiae Cortex (EC) and twenty-four extracts of Eucommiae Folium (EF) under optimal conditions. It was demonstrated that thirteen and twelve substances were possible chemical markers for EC and EF male and female discrimination and that the level of these markers -chlorogenic acid and protocatechuic acid -was many times higher in male than in female. This approach offered a reference for quality control and precise treatment of male and female E. ulmoides in the clinic.

Optimization of the Zhou Tian Formula extraction technology based on AHP‐CRITIC method and analysis of transfer efficiency of key components based on HPLC fingerprinting

“…The results revealed that the 15 batches of granules could be classified into three distinct categories, consistent with the result of HCA, albeit with a few exceptions. We used a variable importance in projection (VIP) value greater than 1 as the criterion for identifying distinct components, as shown in Figure 6B 31 . Components with VIP values greater than 1 played a crucial role in the OPLS‐DA.…”

“…We used a variable importance in projection (VIP) value greater than 1 as the criterion for identifying distinct components, as shown in Figure 6B. 31 Components with VIP values greater than 1 played a crucial role in of Pueraria Lobata. 25,32 These findings underscore the necessity for stringent quality control measures for fried Astragali Radix and Pueraria Lobata to ensure the stability of ZTF granules.…”