Establishment of Magnetic Microparticles-Assisted Time-Resolved Fluoroimmunoassay for Determinating Biomarker Models in Human Serum

Abstract

In order to early screen and detect suspected biomarkers from pathogens and the human body itself, tracers or reaction strategies that can act as signal enhancers have been proposed forth at purpose. In this paper, we discussed the applicability of magnetic microparticles-assisted time-resolved fluoroimmunoassay (MMPs-TRFIA) for sensitive determination of potential analytes. Hepatitis B e antigen, antibody to hepatitis B surface antigen and free triiodothyronine were used as biomarker models to explore the reliability of the method. By coupling with bioprobes, MMPs were used as immunoassay carriers to capture target molecules. Under optimal condition, assay performance, including accuracy, precision and specificity, was outstanding and demonstrated satisfactory. To further evaluate the performance of the MMPs-TRFIA in patients, a total of 728 serum samples from hospital were analyzed for three biomarkers in parallel with the proposed method and chemiluminescence immunoassay kit commercially available. Fairly good agreements are obtained between the two methods via data analysis. Not only that but the reliability of MMPs-TRFIA has also been illustrated by three different reaction models. It is confirmed that the novel method modified with MMPs has been established and showed great potential applications in both biological detection and clinical diagnosis, including big molecule protein and low molecular weight haptens.

Detection of Carbohydrate Antigen 50 Based on a Novel Miniaturized Chemiluminescence Analyzer Enables Large-Scale Cancer Early Screening in Grassroots Community

“…( Supplementary Figure S1 ). Second, the immunoconjugate of MP-coated anti-CA50 antibodies (MPs-Ab) was prepared according to the previously reported method ( Ren et al, 2015 ) with a little modification. ALP-labeled CA50 antibodies (ALP-Ab) were prepared, as described previously ( Du Ye and Peng, 2015 ), with minor modifications.…”

Development of Monoclonal Hybridoma Cell Lines and Extracting Antibody Against Fummonisin B1

“…To detect the existence of FB 1 , immunochemical method is often the ideal technique because of its high sensitivity as well as the specificity of the antibody, therefore, this research work is to develop anti FB 1 monoclonal antibody with hybridoma technique and then create the sensitive immunological method based on time-resolved fluoroimmunoassay (TRFIA) [11] used for FB 1 detection in food, so as to provide credible monitoring tools for the food safety risk monitoring.…”