Establishment of intraperitoneal germ cell transplantation for critically endangered Chinese sturgeon Acipenser sinensis

Ye, Huan; Li, Chuangju; Yue, Huamei; Du, Hao; Yang, Xiaoge; Yoshino, Tasuku; Hayashida, Takao; Takeuchi, Yutaka; Wei, Qiwei

doi:10.1016/j.theriogenology.2017.02.009

Cited by 60 publications

(35 citation statements)

References 39 publications

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“…At present, several different approaches to germ cell transplantation techniques, either within the same species (allogenic) or between two different species (xenogenic), have been established in fish (Yoshizaki et al 2011;Lacerda et al 2013) and are widely used to study germ cell biology, such as the function of germline stem cells (Yoshizaki et al 2012;Hayashi et al 2014). These techniques also have applications in the conservation of endangered species (Lee et al 2015;Pšenička et al 2015;Lee and Yoshizaki 2016;Ye et al 2017), the propagation of valuable fishes with economically useful traits (Morita et al 2012(Morita et al , 2015Silva et al 2016), the cryopreservation of scientific bioresources (Lee et al 2013(Lee et al , 2016aSeki et al 2017), and the efficient production of genetically engineered fish (Tonelli et al 2017). For the purpose of germ cell transplantation experiments, sterilized recipients are useful for obtaining successful colonization of donor germ cells in recipient genital ridges and gonads, and the efficient production of donor-derived gametes without contamination of recipient-derived gametes.…”

Section: Discussionmentioning

confidence: 99%

Hybrid Sterility in Fish Caused by Mitotic Arrest of Primordial Germ Cells

Yoshikawa

Ino

et al. 2018

Genetics

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Sterility in hybrid animals is widely known to be due to a cytological mechanism of aberrant homologous chromosome pairing during meiosis in hybrid germ cells. In this study, the gametes of four marine fish species belonging to the Sciaenid family were artificially fertilized, and germ cell development was examined at the cellular and molecular levels. One of the intergeneric hybrids had gonads that were testis-like in structure, small in size, and lacked germ cells. Specification of primordial germ cells (PGCs) and their migration toward genital ridges occurred normally in hybrid embryos, but these PGCs did not proliferate in the hybrid gonads. By germ cell transplantation assay, we showed that the gonadal microenvironment in hybrid recipients produced functional donor-derived gametes, suggesting that the germ cell-less phenotype was caused by cell autonomous proliferative defects of hybrid PGCs. This is the first evidence of mitotic arrest of germ cells causing hybrid sterility in animals.

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Section: Discussionmentioning

confidence: 99%

Hybrid Sterility in Fish Caused by Mitotic Arrest of Primordial Germ Cells

Yoshikawa

Ino

et al. 2018

Genetics

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“…Transplantation of fish germ cells offers a novel strategy for rapid breeding and seed conservation 37 , 38 , but this strategy is unfeasible for industrialized application due to low transplantation efficiency (which may be enhanced depending on the donor fish cells and the quality of the recipient fish). Currently, multiple recipient fish species lack a sterile line 39 , 40 . Conventionally physical and chemical approaches have been employed, but they have either failed to completely eliminate the germ cells or resulted in substantial variability of outcome, which greatly increases the workload for subsequent screening.…”

Section: Discussionmentioning

confidence: 99%

Generation of all-male-like sterile zebrafish by eliminating primordial germ cells at early development

Zhou

Yuan

Wang

et al. 2018

Sci Rep

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Production of all-male and sterile fish may not only substantially improve yield but also be crucial for the application of genome modified species in aquaculture. Previously, it was reported that the fish lacking primordial germ cells (PGCs) becomes infertile, and nitroreductase, an enzyme converting non-toxic metronidazole (MTZ) into toxic metabolites, induces targeted toxicity to kill the cells expressing it. In this study, we generated a transgenic zebrafish line of Tg(nanos3:nfsB-mCherry-nanos3 3′UTR) in which the NfsB nitroreductase is solely expressed in PGCs. Treating the embryos derived from the female transgenic zebrafish with MTZ from 0 through 2 dpf (days post fertilization), we found that the germ cells were completely eliminated in the ones older than 2.5 dpf. At 20 dpf, the MTZ-treated juvenile had no germ cells in their gonads. At 100 dpf, the MTZ-treated adult exhibited male-like morphology and showed normal mating behaviors although they had no germ cells but only supporting cells in their gonads. Taken together, our results demonstrated that conditional elimination of PGCs during early development make the zebrafish male-like and infertile. It may provide an alternative strategy to make sterile and all-male farmed fish that is good for increasing aquaculture yield and preventing the genome modified species from potential ecological risks.

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“…However, no chimeric females were observed, which could be the result of the development of a rudimentary ovary in the female hybrid, making the colonization and/or proliferation of transplanted ovarian cells difficult (Wong et al 2011). On the contrary, Ye et al (2017) demonstrated that ovarian germ cells harvested from the critically endangered Chinese sturgeons A. sinensis were able to colonize the genital ridge in 40% of the transplanted larvae of the closer related Dabry's sturgeon A. dabryanus.…”

Section: Oogonia Transplantationmentioning

confidence: 97%

“…However, since germline transmission rate to offspring was considered low because of niche competition between donor and endogenous germ cells, subsequent studies commonly used sterile hosts, such as Okutsu et al (2007) that produced trout fry from triploid sterile salmon. Moreover, thinking about fish conservation, spermatogonial transplantations were successfully conducted between two different species, such as the flatifish Senegalese sole (donor) and Scophthalmus maximus (host) (Pacchiarini et al 2014), between the sturgeons Siberian sturgeon Acispenser baerii (donor) and the starlet sturgeon Acispenser ruthenus (host) (Pšenička et al 2015), between the salmonids the Rainbow trout Onchorhynchus mykiss (donor) and the salmon (host) (Yoshizaki et al 2016), between the tiger puffer Takifugu rubripes (donor) and the grass puffer Takifugu niphobles (host) (Hamasaki et al 2017), and between the Chinese Acipenser sinensis (donor) and Acipenser dabryanus Dabry's (host) sturgeons (Ye et al 2017).…”

Section: Pgc Transplantation Between Embryosmentioning

confidence: 99%

Biotechnology applied to fish reproduction: tools for conservation

Siqueira‐Silva

Saito

Santos-Silva

et al. 2018

Fish Physiol Biochem

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This review discusses the new biotechnological tools that are arising and promising for conservation and enhancement of fish production, mainly regarding the endangered and the most economically important species. Two main techniques, in particular, are available to avoid extinction of endangered fish species and to improve the production of commercial species. Germ cell transplantation technology includes a number of approaches that have been studied, such as the transplantation of embryo-to-embryo blastomere, embryo-to-embryo differentiated PGC, larvae to larvae and embryo differentiated PGC, transplantation of spermatogonia from adult to larvae or between adults, and oogonia transplantation. However, the success of germ cell transplantation relies on the prior sterilization of fish, which can be performed at different stages of fish species development by means of several protocols that have been tested in order to achieve the best approach to produce a sterile fish. Among them, fish hybridization and triploidization, germline gene knockdown, hyperthermia, and chemical treatment deserve attention based on important results achieved thus far. This review currently used technologies and knowledge about surrogate technology and fish sterilization, discussing the stronger and the weaker points of each approach.

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Establishment of intraperitoneal germ cell transplantation for critically endangered Chinese sturgeon Acipenser sinensis

Cited by 60 publications

References 39 publications

Hybrid Sterility in Fish Caused by Mitotic Arrest of Primordial Germ Cells

Hybrid Sterility in Fish Caused by Mitotic Arrest of Primordial Germ Cells

Generation of all-male-like sterile zebrafish by eliminating primordial germ cells at early development

Biotechnology applied to fish reproduction: tools for conservation

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