Establishment of induced pluripotent stem cells from normal B cells and inducing AID expression in their differentiation into hematopoietic progenitor cells

Abstract

Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs)1 by exogenous expression of reprogramming factors (Yamanaka factors) such as Oct4, Sox2, Klf4 and c-Myc. Since the invention of this method, iPSCs have been established from a variety of somatic cells not only for regenerative medicine but also for studies of the pathogenesis of inherited genetic disease 2-5 or neoplasms [6][7][8][9] . In term of the establishment of iPSCs from blood cells, the T cells that were derived from antigen-specific CD8 + T cells in an HIV-1-infected patient 10 , or from mature cytotoxic T cells that were specific for the melanoma epitope MART-1 11 , were reprogramed into iPSC, and were then re-differentiated into CD8+ cells that possessed antigen-specific killing activity for treatment of patients with AIDS or melanoma, respectively. The important point of these studies is that the rearrangement of the T cell receptor (TCR) of the established T cell derived iPSC (TiPSC) was the same as that