Establishment of in vitro regeneration system for Acaciella angustissima (Timbe) a shrubby plant endemic of México for the production of phenolic compounds

Alonso-Herrada, Jannette; Rico-Reséndiz, Félix; Campos-Guillén, Juan; Guevara-González, Ramón Gerardo; Torres-Pacheco, Irineo; Cruz-Hernández, Andrés

doi:10.1016/j.indcrop.2016.03.040

Cited by 10 publications

(1 citation statement)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(1) Multiple shoots were regenerated from the nodal explants on the medium supplemented with 2, 4-D in all the treatments in combination with BAP, TDZ, IAA (Indole acetic acid), and NAA along with activated charcoal used as an antioxidant source in some replicates. The regeneration frequency of multiple shoots was calculated by using the following formula (Alonso-Herrada et al, 2016).…”

Section: Callogenesis and Organogenesismentioning

confidence: 99%

Multiple shoot regeneration, establishment of callus and cell suspension cultures in Salacia macrosperma Wight

Mahendra¹,

Manasa²,

Murali³

et al. 2020

Biotechnology Research and Innovation

View full text Add to dashboard Cite

Salacia macrosperma Wight. -a potent medicinal plant facing the verge of rare and endemic status to the Western Ghat of southern India. Effective protocol has been established for induction of callus and multiple shoot regeneration using leaf and nodal explants on MS medium fortified with various plant growth regulators like 2,4-dichlorophenoxyacetic acid (2,4-D), benzyl amino purine (BAP), thidiazuron (TDZ), Indole acetic acid (IAA), Kinetin (Kn), Naphthalene acetic acid (NAA) and Indole butyric acid (IBA). Leaf explants produced more calli (98.33%) than nodal explants at 2.5 + 1.5 mg L -1 of 2, 4-D and BAP in combination. MS medium with 1.5 mg L -1 of 2, 4-D, 2.0 mg L -1 of BAP and 1.5 mg L -1 of TDZ along with 1% activated charcoal was apt for multiple shoot regenerations (93.33%) from nodal explants with embryogenic callus. Rhizogenesis was achieved in liquid MS medium supplemented with 1.0 mg L -1 of IAA. Friable callus when agitated in liquid MS medium at 100 rpm embryogenic callus followed by morphological changes were observed. Cytogenetic variations such as multinucleate, multinucleolate, cytodifferentiation, chromosomal bridges were noticed, besides normal dividing stages. Anatomical features were observed by microtome sections of the embryogenic callus revealed the presence of active centers, embryoids, and shoot bud formation. Further, by scanning electron micrograph (SEM) analysis of embryogenic callus different stages of morphogenic developmental features were recorded.

show abstract

Section: Callogenesis and Organogenesismentioning

confidence: 99%

Multiple shoot regeneration, establishment of callus and cell suspension cultures in Salacia macrosperma Wight

Mahendra¹,

Manasa²,

Murali³

et al. 2020

Biotechnology Research and Innovation

View full text Add to dashboard Cite

show abstract

Effect of the genotype, explant source and culture medium in somatic embryogenesis and organogenesis in Vaccaria hispanica (Mill.) Rauschert

Bedir

Ari

Vural

et al. 2022

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Vaccaria hispanica is an interesting species with attractive agronomic properties and a wealth of valuable bioactive compounds, potentially useful for many different purposes. Surprisingly, the number of studies focused on the development of in vitro tools for a rapid production of clonal populations is extremely limited. In the present study, two wild Turkish genotypes, previously characterized as high starch and saponin producers, are used to explore the possibilities of regenerating clonal plants through somatic embryogenesis and organogenesis. This work investigates the independent effects of genotype, type of explant and composition of the culture medium, and the interactions among them, in the growth and proliferation of calli from the explants, and the induction of somatic embryogenesis and organogenesis fron the callus surface. Some of the interactions were found significant to promote these processes. V. hispanica proved to be especially responsive for callus induction from all the explants tested. Particular explant types and combinations of plant growth regulators have been identified as especially suitable to induce the different morphogenic processes. V. hispanica is remarkably prone to produce adventitious roots, which may be a problem when trying to induce somatic embryogenesis or shoot organogenesis. However, this can be exploited to develop a convenient system for in vitro secondary metabolite production.

show abstract

Biotic and Abiotic Factors to Increase Bioactive Compounds in Fruits and Vegetables

et al. 2017

View full text Add to dashboard Cite

Establishment of in vitro regeneration system for Acaciella angustissima (Timbe) a shrubby plant endemic of México for the production of phenolic compounds

Cited by 10 publications

References 27 publications

Multiple shoot regeneration, establishment of callus and cell suspension cultures in Salacia macrosperma Wight

Multiple shoot regeneration, establishment of callus and cell suspension cultures in Salacia macrosperma Wight

Effect of the genotype, explant source and culture medium in somatic embryogenesis and organogenesis in Vaccaria hispanica (Mill.) Rauschert

Biotic and Abiotic Factors to Increase Bioactive Compounds in Fruits and Vegetables

Contact Info

Product

Resources

About