Establishment of human iPSC line NCCSi003-A from CD34 + cells of peripheral blood collected during apheresis of healthy donor from Indian ethnicity

Fernandes, Sophia; Tembe, Shruti; Shinde, Prajakta; Melinkeri, Sameer; Kale, Vaijayanti; Limaye, Lalita

doi:10.1016/j.scr.2017.12.011

Cited by 4 publications

(3 citation statements)

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“…The cascade filtration, and extracorporeal photopheresis, were reported only from one blood centre each. The centres from India are increasingly reporting the use of apheresis for ABO‐incompatible solid organ transplant, 17–20 therapeutic cytoreduction, 21,22 peripheral blood stem cell (PBSC) harvest, 23 and regenerative medicine for the establishment of human‐induced pluripotent stem cell lines 24 …”

Section: Discussionmentioning

confidence: 99%

Therapeutic apheresis and non‐blood donor related apheresis current practices at various blood centres of healthcare organisations of India: A brief online survey

Kumawat

Tripathi

Patidar

2021

Transfusion Medicine

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Objectives To determine the variability in therapeutic apheresis (TA) and non‐blood donor related apheresis practices, and the extent of expertise and knowledge of blood centre staff. Background Apheresis activity that was earlier limited to therapeutic plasma exchange (TPE) and donor apheresis at few centres in India has seen remarkable surge involving many centres practising TA and non‐blood donor related apheresis. The decentralised transfusion medicine practice in country has resulted in wide variability of knowledge and practice of TA. An online survey was conducted to achieve study objectives. Study design and methods A 22 questionnaire survey was sent to the 215 blood centres through e‐mail link focussing on three aspects; basic information of the participating centres, details of TA procedures and education and training levels of the staff. Results Majority (71.9%) of centres were teaching institutions among analysed 57 centres. TPE (85.9%) and therapeutic cytapheresis (71.9%) were the most common TA procedures. The clinical haematology (68.4%) followed by neurology (64.9%) were the specialities utilising TA. The 64.9% centres used continuous flow cell separator and central venous access (52%) was preferred vascular access. A combination of normal saline, fresh frozen plasma and 5% albumin replacement fluid was first choice. Doctors involved in TA were trained in apheresis during their MD/DNB degree, but no structured training program existed for other category of staff. Conclusion There was a wide variability in TA practice in India and a dedicated training program for all categories of staff was emphasised by majority of participants.

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Section: Discussionmentioning

confidence: 99%

Therapeutic apheresis and non‐blood donor related apheresis current practices at various blood centres of healthcare organisations of India: A brief online survey

Kumawat

Tripathi

Patidar

2021

Transfusion Medicine

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“…In our lab, while reprogramming CD34 + cells from CB/apheresis sample, a few iPSC lines that displayed normal karyotype were established (Fernandes et al, ; Fernandes, Shinde, et al, ; Fernandes, Tembe, et al, ). During this process, one of the transfections resulted in development of a line that showed full trisomy of Chromosome 1 and a deletion of small fragment q23 to qter of Chromosome 11.…”

Section: Discussionmentioning

confidence: 99%

“…We have developed a few induced pluripotent stem cell (iPSCs) lines from cord blood (CB)/apheresis‐derived CD34 + cells of Indian ethnicity, using episomal vectors (Fernandes et al, ; Fernandes, Shinde, et al, ; Fernandes, Tembe, et al, ). While generating such lines, in one of the lines, we found an abnormality in Chromosomes 1 and 11.…”

Section: Introductionmentioning

confidence: 99%

Improved neural differentiation of normal and abnormal induced pluripotent stem cell lines in the presence of valproic acid

Fernandes

Vinnakota

Kumar

et al. 2019

J Tissue Eng Regen Med

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During the generation of induced pluripotent stem cell (iPSC) lines from cord blood CD34+ cells, a line having complete trisomy of Chromosome 1 and deletion of q23 to qTer of Chromosome 11 was accidentally developed in our lab. The abnormality was consistently detected even at higher passages. These chromosomal anomalies are known to manifest neurological developmental defects. In order to examine if such defects occur during in vitro differentiation of the cell line, we set up a protocol for neural differentiation. Valproic acid (VPA) was earlier reported by us to enhance neural differentiation of placental mesenchymal stem cells. Here, we induced normal and abnormal iPSC lines to neural lineage with/without VPA. Neural differentiation was observed in all four sets, but for both the iPSCs lines, VPA sets performed better. The characteristics tested were morphology, neural filament length, detection of neural markers, and electrophysiology. In summary, the karyotypically abnormal line exhibited efficient neural differentiation. This iPSC line may serve as a useful tool to study abnormalities associated with trisomy 1 and deletion of q23 to qTer of Chromosome 11.

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Catalase incorporation in freezing mixture leads to improved recovery of cryopreserved iPSC lines

et al. 2019

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Establishment of human iPSC line NCCSi003-A from CD34 + cells of peripheral blood collected during apheresis of healthy donor from Indian ethnicity

Cited by 4 publications

References 1 publication

Therapeutic apheresis and non‐blood donor related apheresis current practices at various blood centres of healthcare organisations of India: A brief online survey

Therapeutic apheresis and non‐blood donor related apheresis current practices at various blood centres of healthcare organisations of India: A brief online survey

Improved neural differentiation of normal and abnormal induced pluripotent stem cell lines in the presence of valproic acid

Catalase incorporation in freezing mixture leads to improved recovery of cryopreserved iPSC lines

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