Establishment of Human Colon Cancer Cell Lines from Fresh Tumors versus Xenografts: Comparison of Success Rate and Cell Line Features

Abstract

Obtaining representative human colon cancer cell lines from

Newly characterised ex vivo colospheres as a three-dimensional colon cancer cell model of tumour aggressiveness

“…XenoCT320 xenograft, CT320 and CT320X6 cell lines have been previously described (Dangles-Marie et al, 2007). For xenograft passage, tumour fragments were subcutaneously grafted in the interscapular region into 5-week-old athymic nude female mice (Harlan, Winkelmann, Germany) bred and maintained in specified pathogen-free conditions (protocol approval no P2.VDM.026.07, local ethical committee on animal experiments, CREEA René Descartes, Paris, France).…”

“…We refer to these 3D structures as colospheres. To further characterise these colospheres, we used a human colon cancer xenograft, XenoCT320, and the derived paired colon cancer cell lines, CT320X6 and CT320 (Dangles-Marie et al, 2007). Colospheres generated from xenograft tissue and spheroids formed on agarose from colon cancer cell lines were then studied in parallel.…”

Explant culture of sarcoma patients' tissue

“…35 Third, patient material could initially be expanded as PDX in mice that markedly increased the subsequent success in explant culture of colon cancer and hepatocellular carcinoma samples (Table 4). 34,39 In conclusion, the here described explant technique proved that patient-derived cell lines from different kinds of sarcoma entities can be established for use in preclinical studies and in personalized medicine.…”

“…In comparison, cells derived from metastatic colon cancer could not be established by the explant technique and instead required patient-derived xenograft (PDX) transplantation, indicating an inverse relationship between success and malignancy. 34 In melanoma, success rates were lower in slow cycling cells, which needed further enrichment by selective trypsinization. A slow cycling phenotype is correlated with a mesenchymal expression profile resembling de-differentiated melanoma and MAPK pathway inhibitor resistance (personal communication).…”

Cancer of mice and men: old twists and new tails

“…Mice have also been used to assess the protective effects of various treatments including different diets. Green tea, for example, was shown to protect Apc Min mice from 12 L van der Weyden and DJ Adams Cancer of mice and men: old twists and new tails 13 intestinal adenomagenesis [81]. Olive oil was shown to have a similar effect [82].…”