Establishment of Higher Passage PER.C6 Cells for Adenovirus Manufacture

Berdichevsky, Marina; Gentile, Marie-Pierre; Hughes, Benjamin S.; Meis, Peter; Peltier, Joseph; Blumentals, I. I.; Auniņš, John G.; Altaras, Nedim Emil

doi:10.1021/bp070258u

Cited by 21 publications

(23 citation statements)

References 35 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is a particularity of influenza virus that is most probably associate with the ability of the virus to induce cell apoptosis 14, 66. In general, other viruses involve an increase of the cell population size 67–70. Detailed analyses of the evolution of the two other dielectric parameters are far beyond the scope of this work, however, it is important to underline that both of them were identified as changing during a viral infection and production 38, 67, 71, 72…”

Section: Resultsmentioning

confidence: 95%

Real‐time monitoring of influenza virus production kinetics in HEK293 cell cultures

Petiot

Kamen

2012

Biotechnology Progress

View full text Add to dashboard Cite

There is an increased interest from the vaccine industry to use mammalian cell cultures for influenza vaccine manufacturing. Therefore, it became important to study the influenza infection mechanism, the viral–host interaction, and the replication kinetics from a bioprocessing stand point to maximize the influenza viral production yield in cell culture. In the present work, influenza replication kinetics was studied in HEK293 cells. Two infection conditions were evaluated, a low (0.01) and a high multiplicity of infection (1.0). Critical time points of the viral production cycle (infection, protein synthesis, viral assembly and budding, viral release, and host‐cell death) were identified in small‐scale cell cultures. Additionally, cell growth, viability, and viral titers were monitored in the viral production process. The infection state of the cultivated cell population was assessed by influenza immunolabeling throughout the culture period. Influenza virus production kinetics were also on‐line monitored by dielectric spectroscopy and successfully correlated to real‐time capacitance measures. Overall, this work provided insights into the mechanisms associated with the infection of human HEK293 cell line by the influenza virus and demonstrated, once again, the usefulness of multifrequency scanning permittivity for in‐line monitoring and supervision of cell‐based viral production processes. Published 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2013

show abstract

Section: Resultsmentioning

confidence: 95%

Real‐time monitoring of influenza virus production kinetics in HEK293 cell cultures

Petiot

Kamen

2012

Biotechnology Progress

View full text Add to dashboard Cite

show abstract

“…In our system, we utilized the FISP® probe to acquire cell free sterile samples from the reactor, and the sample was processed and delivered to an HILIC‐UPLC column using a μSI system after a series of processing steps to monitor glycosylation changes in a fed‐batch culture. An earlier study utilized the FISP® probe in a fed‐batch culture of PER.C6 cells in conjunction with a YSI2700 unit to measure real‐time glucose and lactate measurements (Berdichevsky et al, ). In other reports (Larson et al, ; Maranga and Goochee ), glutamate and glutamine, in addition to glucose and lactate, were monitored using YSI2700 units.…”

Section: Discussionmentioning

confidence: 99%

A framework for real‐time glycosylation monitoring (RT‐GM) in mammalian cell culture

Tharmalingam

Callahan

et al. 2015

Biotech & Bioengineering

View full text Add to dashboard Cite

Glycosylation is a critical characteristic of biotherapeutics because of its central role in in vivo efficacy. Multiple factors including medium composition and process conditions impact protein glycosylation and characterizing cellular response to these changes is essential to understand the underlying relationships. Current practice typically involves glycosylation characterization at the end of a fed-batch culture, which in addition to being an aggregate of the process, reflects a bias towards the end of the culture where a majority of the product is made. In an attempt to rigorously characterize the entire time-course of a fed-batch culture, a real-time glycosylation monitoring (RT-GM) framework was developed. It involves using the micro sequential injection (μSI) system as a sample preparation platform coupled with an ultra-performance liquid chromatography (UPLC) system for real-time monitoring of the antibody glycan profile. Automated sampling and sample preparations were performed using the μSI system and this framework was used to study manganese (Mn)-induced glycosylation changes over the course of a fed-batch culture. As expected, Mn-supplemented cultures exhibited higher galactosylation levels compared to control while the fucosylation and mannosylation were consistent for both supplemented and control cultures. Overall, the approach presented in the study allows real time monitoring of glycosylation changes and this information can be rapidly translated into process control and/or process optimization decisions to accelerate process development.

show abstract

“…Certain attributes of Ad5 vectors make them attractive for use as nasal vaccine platforms. (i) Replication-competent adenovirus (RCA)-free Ad5-vectored vaccines can be manufactured in PER.C6 cells quickly using disposable manufacturing technologies (17,18,22).…”

Section: Discussionmentioning

confidence: 99%

An Adenovirus-Vectored Nasal Vaccine Confers Rapid and Sustained Protection against Anthrax in a Single-Dose Regimen

Zhang¹,

Jex²,

Feng³

et al. 2013

Clin Vaccine Immunol

View full text Add to dashboard Cite

cBacillus anthracis is the causative agent of anthrax, and its spores have been developed into lethal bioweapons. To mitigate an onslaught from airborne anthrax spores that are maliciously disseminated, it is of paramount importance to develop a rapidresponse anthrax vaccine that can be mass administered by nonmedical personnel during a crisis. We report here that intranasal instillation of a nonreplicating adenovirus vector encoding B. anthracis protective antigen could confer rapid and sustained protection against inhalation anthrax in mice in a single-dose regimen in the presence of preexisting adenovirus immunity. The potency of the vaccine was greatly enhanced when codons of the antigen gene were optimized to match the tRNA pool found in human cells. In addition, an adenovirus vector encoding lethal factor can confer partial protection against inhalation anthrax and might be coadministered with a protective antigen-based vaccine.

show abstract

Establishment of Higher Passage PER.C6 Cells for Adenovirus Manufacture

Cited by 21 publications

References 35 publications

Real‐time monitoring of influenza virus production kinetics in HEK293 cell cultures

Real‐time monitoring of influenza virus production kinetics in HEK293 cell cultures

A framework for real‐time glycosylation monitoring (RT‐GM) in mammalian cell culture

An Adenovirus-Vectored Nasal Vaccine Confers Rapid and Sustained Protection against Anthrax in a Single-Dose Regimen

Contact Info

Product

Resources

About