Establishment of hematopoietic tissue primary cell cultures from the giant freshwater prawn Macrobrachium rosenbergii

Thansa, Kwanta; Kruangkum, Thanapong; Pudgerd, Arnon; Chaichandee, Lamai; Amparyup, Piti; Suebsing, Rungkarn; Chotwiwatthanakun, Charoonroj; Vanichviriyakit, Rapeepun; Sritunyalucksana, Kallaya

doi:10.1007/s10616-021-00451-w

Cited by 3 publications

(1 citation statement)

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“…Activation of the melanization enzymatic cascade in G. mellonella hemocyte cultures is a well-known problem [ 18 ], and growing the cells without the use of melanization inhibitors is a major challenge ( Figure 4 A). This observation has also been reported in cell cultures of other invertebrates that possess hemocytes [ 18 , 19 , 20 ].…”

Section: Resultssupporting

confidence: 80%

A Straightforward Method for the Isolation and Cultivation of Galleria mellonella Hemocytes

Admella

Torrents

2022

IJMS

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Galleria mellonella is an alternative animal model of infection. The use of this species presents a wide range of advantages, as its maintenance and rearing are both easy and inexpensive. Moreover, its use is considered to be more ethically acceptable than other models, it is conveniently sized for manipulation, and its immune system has multiple similarities with mammalian immune systems. Hemocytes are immune cells that help encapsulate and eliminate pathogens and foreign particles. All of these reasons make this insect a promising animal model. However, cultivating G. mellonella hemocytes in vitro is not straightforward and it has many difficult challenges. Here, we present a methodologically optimized protocol to establish and maintain a G. mellonella hemocyte primary culture. These improvements open the door to easily and quickly study the toxicity of nanoparticles and the interactions of particles and materials in an in vitro environment.

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