Establishment of hairy root cultures of Psoralea species

Nguyen, Christophe; Bourgaud, Frédéric; Forlot, P.; Guckert, Armand

doi:10.1007/bf00234375

Cited by 52 publications

(27 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, LBA1S produced about twice as much hyoscyamine as C58A, but seemed to be more sensitive to the changes in sucrose concentration than C58A. Our findings support the results found in Psoralea hairy roots (Nguyen et al 1992), but are in contrast to those of Christen et al (1992), who reported that different sucrose concentrations did not affect the growth of H. albus hairy roots.…”

Section: Effect Of Sucrose and Other Carbon Sourcescontrasting

confidence: 34%

Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus

Oksman-Caldentey

Sev�n

Vanhala

et al. 1994

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

The effect of carbon and nitrogen sources on two well-established hairy root clones, LBA1S and C58A, of Hyoscyamus muticus strain Cairo, were investigated. Both clones exhibited completely different patterns with regards to their growth rate, hyoscyamine accumulation, and fatty acid contents. Clone C58A grew faster and yielded more biomass (17.4 g 1-1, in 21 days), but produced less hyoscyamine. The maximum hyoscyamine content (120 mg 1-1) in clone LBA1S was reached in 28 days. Neither of the clones could use lactose or fructose as the sole carbon source, nor ammonium as the sole nitrogen source. The growth in the medium containing glucose was significantly reduced compared to that containing sucrose. Clone LBA1S was sensitive to the changes in sucrose concentration and an increase in ammonium in the culture medium, whereas C58A tolerated these changes better but was more sensitive to the increase in total nitrogen. Lipid synthesis was active in the exponential growth phase, and the total fatty acid content varied from 5 to 34 mg g-I of dry root material. The major fatty acids were linoleic, palmitic and linolenic. There were considerable differences in the total amount of lipids and in their relative ratios when different nutrients were applied.Abbreviations: DW --dry weight, FA --fatty acids, FFA--free fatty acids, FW --fresh weight

show abstract

Section: Effect Of Sucrose and Other Carbon Sourcescontrasting

confidence: 34%

Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus

Oksman-Caldentey

Sev�n

Vanhala

et al. 1994

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…29 Our findings support the results in Psoralea, 29 Swertia chirata 14 hairy roots, but are in contrast to those of Christen et al, 18 who reported that the growth of the Hyoscyamus albus hairy roots were not affected by different sucrose concentrations. The FW/DW ratio of Datura stramonium hairy root culture is greatly affected by the level of sucrose supplied relative to the total ion content of the medium.…”

supporting

confidence: 89%

Yield enhancement strategies for the production of picroliv from hairy root culture ofPicrorhiza kurroaRoyle ex Benth.

Verma

Singh

Negi

et al. 2015

Plant Signaling & Behavior

View full text Add to dashboard Cite

Fast-growing hairy root cultures of Picrorhiza kurroa induced by Agrobacterium rhizogenes offers a potential production system for iridoid glycosides. In present study we have investigated the effects of various nutrient medium formulations viz B5, MS, WP and NN, and sucrose concentrations (1-8%) on the biomass and glycoside production of selected clone (14-P) of P. kurroa hairy root. Full strength B5 medium was found to be most suitable for maximum biomass yield on the 40th day of culture (GI D 32.72 § 0.44) followed by the NN medium of the same strength (GI D 22.9 § 0.43). Secondary metabolite production was 1.1 and 1.3 times higher in half strength B5 medium respectively in comparison to MS medium. Maximum biomass accumulation along with the maximum picroliv content was achieved with 4% sucrose concentration in basal medium. RT vitamin and Thiamine-HCl effected the growth and secondary metabolite production of hairy roots growing on MS medium but did not show any effect on other media. The pH of the medium played significant role in growth and secondary metabolite production and was found to be highest at pH 6.0 while lowest at pH 3.0 and pH 8.0. To enhance the production of biomass and Picroliv 5 liter working capacity bioreactor was used, 27-fold (324 g FW) higher growth was observed in bioreactor than shake flask and secondary metabolite production was similarly enhanced.

show abstract

“…Strain A4 produced low hairy root induction in comparison to other strains. This could be due to presence of different plasmids in this strain (Nguyen et al 1992).…”

Section: Discussionmentioning

confidence: 93%

A reliable and efficient protocol for inducing genetically transformed roots in medicinal plant Nepeta pogonosperma

Valimehr

Sanjarian

Sohi

et al. 2014

Physiol Mol Biol Plants

View full text Add to dashboard Cite

Nepeta pogonosperma is an important medicinal plant with anti-inflammatory effects. An efficient and reliable transformation system for this plant was developed through optimization of several factors which affected the rate of Agrobacterium rhizogenes mediated transformation. Five bacterial strains, A4, ATCC15834, LBA9402, MSU440 and A13, two explant types, leaves and stems, and several co-cultivation media were examined. The maximum rate of hairy root induction was obtained from stem explants using MSU440 and ATCC15834 bacterial strains. A drastic increase in the frequency of transformation (91 %) was observed when MS medium lacking NH 4 NO 3 , KH 2 PO 4 , KNO 3 and CaCl 2 . Hairy root lines were confirmed by polymerase chain reaction (PCR) using primers of the rolB gene. According to Southern blot analysis, one T-DNA copy was inserted into each of the hairy root lines. In the present study, transgenic hairy roots have been obtained trough genetic transformation by A. rhizogenes harbouring two plasmids, the Ri plasmid and pBI121 binary vector harbouring gus reporter gene. Expression of the gus gene in transgenic hairy root was confirmed by histochemical GUS assay.

show abstract

Establishment of hairy root cultures of Psoralea species

Cited by 52 publications

References 20 publications

Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus

Effect of nitrogen and sucrose on the primary and secondary metabolism of transformed root cultures of Hyoscyamus muticus

Yield enhancement strategies for the production of picroliv from hairy root culture ofPicrorhiza kurroaRoyle ex Benth.

A reliable and efficient protocol for inducing genetically transformed roots in medicinal plant Nepeta pogonosperma

Contact Info

Product

Resources

About