Establishment of Friable Embryogenic (Type Ii) Callus From Immature Tassels of Zea Mays (Poaceae)

Songstad, D. D.; Petersen, William L.; Armstrong, Charles

doi:10.1002/j.1537-2197.1992.tb13651.x

Cited by 26 publications

(8 citation statements)

References 22 publications

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“…Callus induced transformation for maize is restricted because of following reasons: A) the regeneration of plants from non embryogenic callus (Type 1) is very less, 2) the proliferation of embryogenic callus results only from immature embryos. 23 - 25 Immature embryos are the most widely used explants for developing maize transgenics. 26 The difficulties of using immature embryos are unavailability of explants round the year, seasonal variation for pollination and also the requirement of high quality greenhouse facility.…”

Section: Resultsmentioning

confidence: 99%

An efficient and rapid regeneration via multiple shoot induction from mature seed derived embryogenic and organogenic callus of Indian maize (Zea maysL.)

Pathi

Tula

Huda

et al. 2013

Plant Signaling & Behavior

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An efficient method for in vitro micro propagation and genetic transformation of plants are crucial for both basic and applied research. Maize is one of the most important cereal crops around the world. Regeneration from immature embryo is hampered due to its unavailability round the year. On the contrary mature embryo especially tropical maize is recalcitrant toward tissue culture. Here we report a highly efficient regeneration (90%) system for maize by using 2 different approaches i.e., embryogenic and organogenic callus cultures. Seeds were germinated on MS medium supplemented with 5 mg/l 2,4-D and 3 mg/l BAP. Nodal regions of 2 wks old seedlings were longitudinally split upon isolation and subsequently placed on callus initiation medium. The maximum frequency of embryogenic callus formation (90%) was obtained on MS medium supplemented with 2 mg/l 2,4-D and 1 mg/l BAP in the dark conditions. The compact granular organogenic callus formation (85% frequency) was obtained on MS medium supplemented with 2.5 mg/l 2,4-D and 1.5 mg/l BAP at light conditions. MS medium supplemented with 2 mg/l BAP, 1 mg/l Kinetin and 0.5 mg/l NAA promoted the highest frequency of shoot induction. The highest frequency of root formation was observed when shoots were grown on MS medium. The regenerated plants were successfully hardened in earthen pots after adequate acclimatization. The important advantage of this improved method is shortening of regeneration time by providing an efficient and rapid regeneration tool for obtaining more stable transformants from mature seeds of Indian tropical maize cultivar (HQPM-1).

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Section: Resultsmentioning

confidence: 99%

An efficient and rapid regeneration via multiple shoot induction from mature seed derived embryogenic and organogenic callus of Indian maize (Zea maysL.)

Pathi

Tula

Huda

et al. 2013

Plant Signaling & Behavior

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“…Plant regeneration has also been reported from calli initiated from different explants, namely, anthers [2][3][4], glumes [5], immature inflorescence [6], immature tassels [7][8][9], leaf segments [10][11][12], protoplasts [13], seedling segments [14][15][16], shoot tips [17][18][19], shoot apical meristem [20], mature embryos [15,21,22], and immature embryos [23][24][25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Coleoptilar node - A season-independent explant source for in vitro culture in maize (Zea mays L.)

2018

J App Biol Biotech

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In vitro plant regeneration is preliminary requisite in the process of obtaining transgenic plants. Somatic embryogenesis is the most frequently used method of in vitro plant regeneration in maize. In this study, a reliable and reproducible maize regeneration method has been standardized using mature embryos. Inspite, the immature embryos are most suitable explants for somatic embryogenesis, obtaining these explants continuously is a laborious and tedious process, especially in summer. Hence, alternate explants of maize for in vitro regeneration have been chosen from different parts of embryonic axis. This study provides an evaluation of inbred lines and effect of hormonal combinations on total and embryogenic callus induction, their growth rate, and plantlet regeneration. Coleoptilar nodal explants from four inbred lines were cultured on different concentrations and combinations of 2,4-dichloro phenoxyacetic acid (2,4-D) along with kinetin (Kn). Explants grown on Murashige and Skoog medium supplemented with 2,4-D and Kn at a concentration of 5 and 1 mg/L, respectively, were found to be the best one for yielding a high frequency of embryogenic callus. Inbred line, NM81A was found to be the best one for in vitro culture among the four lines used. Combination of 6-benzylaminopurine and Kn at a concentration of 1 mg/L each promoted the highest frequency of shoot induction.

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“…Experiments were designed based on previous studies undertaken on coconut and other species [red goosefoot ( Chenopodium rubrum L.), date palm, sorghum ( Sorghum bicolor L. Moench), maize ( Zea mays L.), and cassava ( Manihot esculenta Crantz)]. The factors that had been shown to affect friable callus or friable EC formation in these studies were the medium form (liquid or semi-solid) [ 13 ], the frequency of subculture [ 10 , 14 ], the concentration of 2,4-dichlorophenoxyacetic acid (2,4-D) during subculture [ 15 , 16 ], the nitrogen level and form [ 12 ], the addition of an amino acid mixture [ 17 ] or L-proline [ 18 , 19 ], and the use of reduced nutrient content in the medium [ 20 ]. Hence, these factors were considered in the present study with the aim to produce friable EC in coconut.…”

Section: Introductionmentioning

confidence: 99%

Coconut Callus Initiation for Cell Suspension Culture

Kong

Biddle

Kalaipandian

et al. 2023

Plants

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The development of a cell suspension culture system for the scaling up of coconut embryogenic callus (EC) production would drastically improve efforts to achieve the large-scale production of high-quality clonal plantlets. To date, the hard nature of coconut EC appeared to be the main constraint for developing cell suspension cultures. Hence, this study attempted to acquire friable EC through the following approaches: The manipulation of (1) medium type and subculture frequency, (2) a reduced 2,4-dichlorophenoxy acetic acid concentration during subculture, (3) the nitrate level and the ammonium-to-nitrate ratio, and the addition of amino acid mixture, (4) the addition of L-proline, and (5) the reduction of medium nutrients. Unfortunately, none of these culture conditions produced friable coconut EC. Even though friable EC was not achieved via these approaches, some of the conditions were found to influence the formation of compact EC, therefore these results are important for further studies focused on somatic embryogenesis in coconut and other species.

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Establishment of Friable Embryogenic (Type Ii) Callus From Immature Tassels of Zea Mays (Poaceae)

Cited by 26 publications

References 22 publications

An efficient and rapid regeneration via multiple shoot induction from mature seed derived embryogenic and organogenic callus of Indian maize (Zea maysL.)

An efficient and rapid regeneration via multiple shoot induction from mature seed derived embryogenic and organogenic callus of Indian maize (Zea maysL.)

Coleoptilar node - A season-independent explant source for in vitro culture in maize (Zea mays L.)

Coconut Callus Initiation for Cell Suspension Culture

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