Establishment of forskolin yielding transformed cell suspension cultures of Coleus forskohlii as controlled by different factors

Mukherjee, Swapna; Ghosh, Biswajit; Jha, Sumita

doi:10.1016/s0168-1656(99)00181-9

Cited by 40 publications

(14 citation statements)

References 26 publications

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“…The fact that forskolin directly activates adenyl cyclase and thus the modulation of cAMP could underlie the diversity of different traditional uses of forskolin-containing species of Plectranthus, such as Plectranthus barbatus. For example, activation of adenyl cyclase can stimulate gastric secretions and the presence of forskolin in Plectranthus barbatus could explain why extracts from the plant are used for the treatment of digestive disorders (Bhat et al, 1979(Bhat et al, , 1980Hersey et al, 1983;Mukherjee et al, 2000). Forskolin is known to directly activate the catalytic subunit of adenylate cyclases in smooth muscle cells, an action that is made possible by enhanced calcium intake (Metzger and Lindner, 1981;Lindner and Metzger, 1983).…”

Section: Chemistry Of Plectranthus Speciesmentioning

confidence: 99%

Plectranthus: A review of ethnobotanical uses

Lukhoba

Simmonds

Paton

2006

Journal of Ethnopharmacology

436

364

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Section: Chemistry Of Plectranthus Speciesmentioning

confidence: 99%

Plectranthus: A review of ethnobotanical uses

Lukhoba

Simmonds

Paton

2006

Journal of Ethnopharmacology

436

364

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“…Taking into account the increasing demand for these products, plant cell culture provides an attractive alternative source that can overcome the limitations of extracting useful metabolites from limited natural resources. Currently, in vitro culture is widely being employed as a model system to investigate the production of specific secondary metabolites as it offers experimental advantages to both basic and applied research and to the development of models with scale-up potential (Buitelaar and Trapmer 1992;Chang and Sim 1995;Mukherjee et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Establishment of callus and cell suspension culture of Scrophularia striata Boiss.: an in vitro approach for acteoside production

2014

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In the present study, a protocol was optimized for establishment of callus and cell suspension culture of Scrophularia striata Boiss. as a strategy to obtain an in vitro acteoside producing cell line for the first time. The effects of growth regulators were analyzed to optimize the biomass growth and acteoside production. The stem explant of S. striata was optimum for callus induction. Modified Murashige and Skoog medium supplemented with 0.5 mg/l naphthalene acetic acid ? 2.0 mg/l benzyl adenine was the most favorable medium for callus formation with the highest induction rate (100 %), the best callus growth and the highest acteoside content (1.6 lg/g fresh weight). Incompact and rapid growing suspension cells were established in the liquid medium supplemented with 0.5 mg/l naphthalene acetic acid ? 2.0 mg/l benzyl adenine. The optimum time of subculture was found to 17-20 days. Acteoside content in the cell suspension was high during exponential growth phase and decreased subsequently at the stationary phase. The maximum content of acteoside (about 14.25 lg/g cell fresh weight) was observed on the 17th day of the cultivation cycle. This study provided an efficient way to further regulation of phenylethanoid glycoside biosynthesis and production of valuable acteoside, a phenylethanoid glycoside, on scale-up in S. striata cell suspension culture.

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“…From several earlier reports on medicinal plants, it was observed that plants with highly expressed secondary pathways are reported to be recalcitrant for regeneration and are difficult for genetic transformation studies, as evident from the case of Plumbago zeylanica (Wei et al 2006), Euphorbia nivulia (Sunandakumari et al 2005), Mentha citrata (Spencer et al 1990), and Coleus forskohlii (Mukherjee et al 2000). These studies indicated that the screening of explants for genetic transformation and regeneration is mandatory.…”

Section: Introductionmentioning

confidence: 99%

An efficient plant regeneration and Agrobacterium-mediated genetic transformation of Tagetes erecta

Gupta

Rahman

2014

Protoplasma

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Tagetes erecta, L. an asteraceous plant of industrial and medicinal value, contains important compounds like pyrethrins, thiophenes and lutein, possessing immense potential for insecticidal, nematicidal and nutraceutical activities. Considering the importance and demand for these natural compounds, genetic manipulation of this crop for better productivity of secondary metabolites holds great significance. A rapid and reproducible direct regeneration and genetic transformation system is the prerequisite for genetic manipulation of any crop. This paper elucidates the establishment of an efficient direct regeneration and transformation protocol of T. erecta using Agrobacterium tumefaciens. Investigation of the effects of different types of explants (Hypocotyls, cotyledonary leaves, rachis and leaf sections) and different BAP and GA3 combinations on the regeneration frequency of T. erecta suggested that the best regeneration frequency (66 %) with an average of 5.08 ± 0.09 shoot buds/explant was observed from hypocotyl explants cultured on media containing 1.5 mg/l BAP and 5 mg/l GA3. The transformation protocol was established using A. tumefaciens strain LBA4404, containing the binary vector pBI121, along with the gusA reporter gene with intron under the transcriptional control of the Cauliflower Mosaic Virus (CaMV) 35S promoter and the neomycin phosphotransferase II (nptII) gene as a kanamycin-resistant plant-selectable marker. Various parameters like optimization of kanamycin concentration (200 mg/l) for selection, standardization of cocultivation time (45 min) and acetosyringone concentration (150 μM) for obtaining higher transformation frequency were established using hypocotyl explants. The selected putative transgenic shoots were subsequently rooted on the Murashige and Skoog medium and transferred to the green house successfully. The plants were characterised by analysing the gus expression, amplification of 600 bp npt II fragment and Southern blot hybridization using the PCR-amplified gusA fragment as probe. The standardised protocol established during the study will open new vistas for genetic manipulation and introduction of desired genes for genetic improvement of T. erecta.

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Establishment of forskolin yielding transformed cell suspension cultures of Coleus forskohlii as controlled by different factors

Cited by 40 publications

References 26 publications

Plectranthus: A review of ethnobotanical uses

Plectranthus: A review of ethnobotanical uses

Establishment of callus and cell suspension culture of Scrophularia striata Boiss.: an in vitro approach for acteoside production

An efficient plant regeneration and Agrobacterium-mediated genetic transformation of Tagetes erecta

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