Establishment of experimental conditions for preserving samples of fish blood for analysis with both comet assay and flow cytometry

Ramsdorf, Wanessa Algarte; Guimarães, Fernando de Souza Fonseca; Ferraro, Marcos Vinícius Mocellin; Gabardo, Juarez; Trindade, Edvaldo da Silva; Cestari, Marta Margarete

doi:10.1016/j.mrgentox.2008.11.010

Cited by 45 publications

(19 citation statements)

References 13 publications

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“…The comet assay was useful to evaluate how chemicals in the water affected A. altiparanae erythrocytes, suggesting that the greatest genotoxic effects originated from agricultural and industrial activities. Moreover, the test is sensitive enough to identify DNA damage that may be repaired (Ramsdorf et al , 2009). …”

Section: Discussionmentioning

confidence: 99%

Genotoxic and mutagenic effects of polluted surface water in the midwestern region of Brazil using animal and plant bioassays

et al. 2016

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This study aimed to evaluate DNA damage in animal and plant cells exposed to water from the Água Boa stream (Dourados, Mato Grosso do Sul, Brazil) by using bioassays, and to identify the chemical compounds in the water to determine the water quality in the area. Through the cytotoxicity bioassay with Allium cepa, using micronucleus test, and comet assay, using Astyanax altiparanae fish, the results indicated that biological samples were genetically altered. Micronuclei were observed in erythrocytes of A. altiparanae after exposure to water from locations close to industrial waste discharge. The highest DNA damage observed with the comet assay in fish occurred with the exposure to water from locations where the presence of metals (Cu, Pb, Cd, Ni) was high, indicating the possibility of genotoxic effects of these compounds. Thus, these results reinforce the importance of conducting genotoxicity tests for developing management plans to improve water quality, and indicate the need for waste management before domestic and industrial effluents are released into the rivers and streams.

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Section: Discussionmentioning

confidence: 99%

Genotoxic and mutagenic effects of polluted surface water in the midwestern region of Brazil using animal and plant bioassays

et al. 2016

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“…One hundred nucleoids were analyzed for each fish according to the visual classification based on the migration of DNA fragments from the nucleus. The results were categorized into classes according to Ramsdorf et al (2009).…”

Section: Biomarkersmentioning

confidence: 99%

Multibiomarker in fish to evaluate a river used to water public supply

et al. 2015

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“…The alkaline comet assay with erythrocytes was performed according to Singh et al (1988), with some modifications described by Ramsdorf et al (2009). Only blood samples with cell viability above 80%, determined by the Trypan blue exclusion method, were used in the comet assay.…”

Section: Genotoxic Analysismentioning

confidence: 99%

Integrated biomarker response index using a Neotropical fish to assess the water quality in agricultural areas

et al. 2014

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Aquatic ecosystems in areas with intense agricultural activity are subject to pesticide contamination, which may compromise the health of the fish. In order to verify the quality of the water and the possible effects of pesticides on fish, a method that combines different biomarker responses into an index named "integrated biomarker response" (IBR) was applied using the biological alterations in the Neotropical fish Astyanax altiparanae. Fish were maintained in situ at five sites along a stream that runs in an agricultural area and in a stream within a forest fragment, considered a reference site. After seven days of exposure the following alterations were observed in fish confined at experimental sites: increased activity of glutathione-S-transferase (GST) and catalase (CAT) and increase in the content of reduced glutathione (GSH) in liver and gills, reduction of acetylcholinesterase (AChE) activity in the brain and muscle, increase in the occurrence of DNA strand breaks and in the frequency of micronuclei (MN) and nuclear abnormalities (ENA) in erythrocytes. The IBR highlighted three sites as the most affected, as the animals confined at these sites showed greater variations in biological responses. The biomarkers most important for the IBR results were GST, AChE, DNA breaks and ENA.Ecossistemas aquáticos inseridos em áreas com intensa atividade agrícola estão susceptíveis à contaminação por pesticidas, os quais podem comprometer a saúde dos peixes. A fim de verificar a qualidade da água e os possíveis efeitos de pesticidas sobre peixes, um método integrando o uso de diferentes biomarcadores, denominado índice integrado de respostas de biomarcadores (IBR), foi aplicado utilizando-se as alterações biológicas avaliadas em vários órgãos do peixe neotropical Astyanax altiparanae. Os peixes foram confinados in situ em cinco pontos ao longo de um ribeirão localizado em área de produção agrícola e em um córrego dentro de um fragmento florestal, considerado um local de referência. Após sete dias foram observadas as seguintes alterações nos peixes confinados nos pontos experimentais: aumento da atividade da glutationa-S-transferase (GST) e catalase (CAT) e aumento do conteúdo de glutationa-reduzida (GSH) em fígado e brânquias, redução da atividade da acetilcolinesterase (AChE) no músculo e cérebro, aumento de quebras no DNA e na frequência de micronúcleos (MN) e alterações nucleares (ENA) em eritrócitos. O IBR destacou três dos pontos estudados como os mais afetados pela contaminação agrícola, uma vez que foram observados nos animais confinados nestes locais maiores variações nas respostas biológicas. Os biomarcadores mais significativos para os resultados de IBR foram a GST, AChE, quebras no DNA e ENA.

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Establishment of experimental conditions for preserving samples of fish blood for analysis with both comet assay and flow cytometry

Cited by 45 publications

References 13 publications

Genotoxic and mutagenic effects of polluted surface water in the midwestern region of Brazil using animal and plant bioassays

Genotoxic and mutagenic effects of polluted surface water in the midwestern region of Brazil using animal and plant bioassays

Multibiomarker in fish to evaluate a river used to water public supply

Integrated biomarker response index using a Neotropical fish to assess the water quality in agricultural areas

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