Establishment of experimental conditions for the rapid detection of mutations in the von Hippel-Lindau gene by parallel temperature gradient gel electrophoresis
Abstract:Renal cell carcinoma (RCC) is the most common adult kidney neoplasm and is present in approximately 3% of all adult malignancies. Mutations of the von Hippel‐Lindau (VHL) gene had been found in 55—70% of sporadic RCC and appear to be a critical event in the pathogenesis of clear‐cell RCC. Several screening procedures for the detection of mutations in the VHL gene such as single‐strand conformation polymorphism analysis, Southern blot and polymerase chain reaction (PCR)‐employing methods had been used. Recently… Show more
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