Establishment of epithelial cell lines from individual rabbit lenses

Reddan, John R.; Friedman, Thomas B.; Mostafapour, M. K.; Sutherland, Steven H.; Bondy, Robert L.; McGee, Stephen J.; Goldenberg, Eric M.

doi:10.1007/bf01666017

Cited by 25 publications

(7 citation statements)

References 8 publications

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“…Lens epithelial cells. Rabbit lens epithelial cells (RLE), N/N1003 (6). and human lens epithelial cells (HLE), HLE B3 (7), were seeded in 25 cm2 Falcon cell culture flasks and were grown to 100% confluency in a 95% air, 5% CO, incubator at 36°C.…”

Section: Methodsmentioning

confidence: 99%

Fluorescence Studies of Lens Epithelial Cells and Their Constituents

Atherton

Lambert

Schultz

et al. 1999

Photochem & Photobiology

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Steady-state and time-resolved fluorescence measurements have been made of human and rabbit lens epithelial cells and their total soluble protein. Excitation at 350 nm results in broad fluorescence spectra peaking at 450 nm and stretching into the visible past 650 nm. The fluorescence excitation spectra peak around 350 nm. We assign the species responsible for this absorption and fluorescence as NADPH. Because the absorption of near-UV light (300-400 nm) is responsible for cell damage and death, we postulate that excited states of NADPH are implicated in the mechanisms of cell damage. Preirradiation with 355 nm light leads to a loss of NADPH fluorescence but with no change in decay kinetics. Possible mechanisms for cell damage are explored.

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Section: Methodsmentioning

confidence: 99%

Fluorescence Studies of Lens Epithelial Cells and Their Constituents

Atherton

Lambert

Schultz

et al. 1999

Photochem & Photobiology

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“…Rabbit lens epithelial cell line N\N 1003A was developed from a four-day-old rabbit (Reddan et al, 1980(Reddan et al, , 1986 and OB3L was from an eight-year-old rabbit (Reddan et al, 1983). Cells were grown in 5 ml of MEM with 50 µg ml −" gentamicin plus 8 % rabbit serum (pH 7n2) in 60i15 mm tissue culture plates in a humidified atmosphere with 5 % CO # at 35n5mC.…”

Section: Rabbit Lens Epithelial Cell Culturementioning

confidence: 99%

Thioltransferase is Present in the Lens Epithelial Cells as a Highly Oxidative Stress-resistant Enzyme

Lou

Wang²,

et al. 1998

Experimental Eye Research

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“…This cell line was established from the lenses of newborn rabbits (Reddan al 1980). The cells remain diploid in long-term culture, exhibit a normal chromosome pattern, and are nontumorogenic in vivo.…”

Section: Methodsmentioning

confidence: 99%

“…The cultures were maintained at 36 "C in a water-saturated atmosphere containing 5% CO, (Reddan et al, 1980). Irradiation mocedures.…”

Section: Methodsmentioning

confidence: 99%

UV‐Induced Changes in Cell Cycle and Gene Expression within Rabbit Lens Epithelial Cells

Sidjanin

Grdina

Woloschak

1996

Photochem & Photobiology

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— Damage to lens epithelial cells is a probable initiation process in cataract formation mediated by UV radiation. In these experiments, we investigated the effects of exposure to 254 nm radiation on cell cycle progression and gene expression in the rabbit lens epithelial cell line N/N1003A. The RNA was harvested at various times following exposure to UV (254 nm) radiation and analyzed by dot‐blot and northern blot hybridizations. These results revealed that during the first 6 h following exposure of the cells to UV, there was, associated with decreasing dose, a decrease in accumulation of transcripts specific for histones H3 and H4 and an increase in the in RNA encoding protein kinase C and β‐ and γ‐actin. Using flow cytometry, we detected an accumulation of cells in G1/S phase of the cell cycle 1 h following exposure to 254 nm radiation. The observed changes in gene expression, especially the decreased accumulation of histone transcripts reported here, may play a role in UV‐induced inhibition of cell cycle progression.

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Establishment of epithelial cell lines from individual rabbit lenses

Cited by 25 publications

References 8 publications

Fluorescence Studies of Lens Epithelial Cells and Their Constituents

Fluorescence Studies of Lens Epithelial Cells and Their Constituents

Thioltransferase is Present in the Lens Epithelial Cells as a Highly Oxidative Stress-resistant Enzyme

UV‐Induced Changes in Cell Cycle and Gene Expression within Rabbit Lens Epithelial Cells

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