Establishment of embryogenic cell suspension cultures and Agrobacterium-mediated transformation in an important Cavendish banana cv. Robusta (AAA)

Ghosh, Antara; Ganapathi, T. R.; Nath, Paras; Bapat, Vishwas A.

doi:10.1007/s11240-009-9507-0

Cited by 47 publications

(30 citation statements)

References 31 publications

(36 reference statements)

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“…Establishment of in vitro propagation and regeneration via somatic embryogenesis also provides a platform for genetic engineering of desired traits. The widely recognized single-cell origin of the transformants generated by somatic embryogenesis route is considered advantageous over the traditional regeneration by organogenesis since chimerism is unlikely to occur (Ghosh et al, 2009). However, monocotyledonous plants are generally known to be recalcitrant towards somatic embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Expression Analysis of a SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) Gene in Curcuma alismatifolia Gagnep.

Sucharitakul¹,

Rakmit²,

Boonsorn³

et al. 2014

JAS

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Somatic embryogenesis provides a useful tool to facilitate efficient mass propagation in plants. The SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) gene serves a fundamentally important role in somatic embryogenesis of many plant species. The isolation of a SERK gene homolog, namely CaSERK, from Curcuma alismatifolia Gagnep. cv. Blue Tung, was reported. Prediction of coding sequence showed that it encoded a protein of 628 amino acids showing high similarity to previously characterized SERK sequences and containing all the features shared by members of the SERK family, including five leucine-rich repeats and the distinctive proline-rich SPP domain. Investigation of CaSERK expression revealed that its transcripts were found throughout the whole somatic embryogenesis process with highest abundance in embryogenic callus. These results indicate that CaSERK might have somatic embryogenesis-associated functions in this economically important ornamental ginger. Detection of CaSERK transcript accumulation in flower and coma bract tissues is suggestive of its additional roles in other developmental signaling pathways.

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Section: Introductionmentioning

confidence: 99%

Isolation and Expression Analysis of a SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) Gene in Curcuma alismatifolia Gagnep.

Sucharitakul¹,

Rakmit²,

Boonsorn³

et al. 2014

JAS

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“…An efficient transformation system using appropriate initial explants is a key in producing transgenic plants (Ghosh et al, 2009). The histochemical Gus assay was performed to assess the success of insertion of the gusA gene into the embryogenic cells using Agrobacteriummediated transformation.…”

Section: Discussionmentioning

confidence: 99%

“…Biotechnology tools such as genetic engineering can offer potentially effective and sustainable solution to the problem of controlling banana parasitic nematodes and banana weevils by transferring important genes into the banana genome without altering the original characteristics of a given cultivar. Agrobacterium-mediated transformation is one of the methods of gene transfer that has been successful in the production of transgenic banana of other internationally important cultivars (Khanna et al, 2004;Ghosh et al, 2009). It is a well-known fact that banana as in many monocotyledonous plants cultivar or variety recalcitrance to tissue culture and Agro-bacterium mediated genetic transformation.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium mediated transformation of banana (Musa sp.) cv. Sukali Ndiizi (ABB) with a modified Carica papaya cystatin (CpCYS) gene

Namuddu¹,

Kiggundu²,

A³

et al. 2013

Afr. J. Biotechnol.

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Conventional banana breeding for pest and disease resistance is a very difficult and slow process due to the limited sources of resistance, sterility of cultivated banana varieties, high polyploidy levels, long cropping cycle and the lack of rapid screening methods. Molecular breeding using the transgenic approach with candidate genes such as cystatins offers an alternative method to banana improvement. Cystatin proteins inhibit the activity of cysteine proteases responsible for the breakdown of dietary proteins in the gut of many pests including nematodes resulting in protein deficiency. In this study, the papaya cystatin gene was introduced into the banana genome. Embryogenic cell suspension (ECS) cultures of the banana cultivar Sukali Ndiizi (ABB) were used as explants material for the successful transformation of banana. The Carica papaya cystatin gene (CpCYS-Mut89) previously modified to improve its inhibitory potential against banana pests was introduced into this cultivar using Agrobacterium tumefaciens, strain LBA4404 and the gus reporter gene was used to observe successful transformation process. We report the successful protocol for routine transformation of this cultivar, which was completed in six months with plant regeneration observed at a frequency of 23%. An additional four months was required to multiply the regenerant lines in order to have at least 20 plants per line for downstream challenging studies. Putatively transgenic plants were analyzed by PCR using hpt and CpCYS-Mut89 specific primers to confirm the presence of transgenes. Out of 28 selected lines, 27 were positive for both hpt and CpCYS-Mut89 transgenes giving 96.4% transformation efficiency. Five lines were then selected on the basis of putative PCR positives and a Southern blot analysis gave hybridization signals with 1 to 4 copy number integration patterns characteristic of Agrobacterium mediated transformation. These results confirm stable gene integration in East African banana cultivar cv. Sukali Ndiizi (genome group ABB) through an efficient Agrobacterium-mediated transformation protocol described for routine use in future improvement of this crop with genes of economic importance.

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“…Genetic transformation using Agrobacterium tumefaciens offers the best solution for crop improvement in banana. Relative success in A. tumefaciens genetic transformation had been achieved in other studies using different cultivars such as Agbagba [3,4], Rasthali, Robusta [5], Grand Naine [6], Cavendish and Lady finger [7] by inserting genes such as Carica papaya cystatin (CpCYS), rice chitinase (RCC) [8] -1,3-glucanase, and Human lysozym (HL) [9].…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium tumefaciens-mediated Transformation of Embryogenic Callus and Somatic Embryos of the Banana cv “Ambon Lumut” (Musa acuminata)

Kusumastuti¹,

Esyantia²,

Dwivany³

2017

KLS

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Banana is one of the major fruit crops, though its conventional breeding has limitations, such as sterility and high polyploidy levels. Biotechnological approach using genetic transformation crop for improvement offers an alternative solution. In this study a protocol was developed for establishing genetic transformation from embryogenic callus and somatic embryos of the banana cv Ambon Lumut. Embryogenic callus was

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Establishment of embryogenic cell suspension cultures and Agrobacterium-mediated transformation in an important Cavendish banana cv. Robusta (AAA)

Cited by 47 publications

References 31 publications

Isolation and Expression Analysis of a SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) Gene in Curcuma alismatifolia Gagnep.

Isolation and Expression Analysis of a SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) Gene in Curcuma alismatifolia Gagnep.

Agrobacterium mediated transformation of banana (Musa sp.) cv. Sukali Ndiizi (ABB) with a modified Carica papaya cystatin (CpCYS) gene

Agrobacterium tumefaciens-mediated Transformation of Embryogenic Callus and Somatic Embryos of the Banana cv “Ambon Lumut” (Musa acuminata)

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