Establishment of DNA barcodes for the identification of the botanical sources of the Chinese ‘cooling’ beverage

Li, Ming; Wong, Ka-Lok; Chan, Wing-Hin; Li, Jie-Xin; But, Paul Pui‐Hay; Cao, Hui; Shaw, Pang‐Chui

doi:10.1016/j.foodcont.2011.12.008

Cited by 16 publications

(8 citation statements)

References 31 publications

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“…In addition, extracts of Flos Lonicerae Japonicae have shown efficacy against various diseases such as H1N1 influenza [4, 7–9], hand-foot-and-mouth disease (HFMD) [4, 10], and severe acute respiratory syndromes, among others [11–13]. In recent years, Flos Lonicerae Japonicae has also become the main material of a Chinese “cooling” beverage [14]. Flos Lonicerae, which comes from L. macranthoides Hand-Mazz, L. fulvotomentosa Hsu et S.C. Cheng, L. confusa (Sweet) DC., and L. hypoglauca Miq., is another medicinal material from Lonicera [6].…”

Section: Introductionmentioning

confidence: 99%

“…Several DNA regions have been evaluated for potential application as DNA barcodes, such as matK , rbcL , psbA-trnH , and ITS2 [26–28]. In the recent years, the DNA barcoding technology has been increasingly used in several fields including species identification [29–33], biodiversity study [34, 35], misidentification detection [36], food safety testing [14], and tackling illegal trade of endangered species [37, 38]. Chen et al suggested that ITS2 can be used as a novel DNA barcode for the identification of medicinal plants and their closely related species [27].…”

Section: Introductionmentioning

confidence: 99%

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Stability and Accuracy Assessment of Identification of Traditional Chinese Materia Medica Using DNA Barcoding: A Case Study on Flos Lonicerae Japonicae

Hou

Song

Shi

et al. 2013

BioMed Research International

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DNA barcoding is a novel molecular identification method that aids in identifying traditional Chinese materia medica using traditional identification techniques. However, further study is needed to assess the stability and accuracy of DNA barcoding. Flos Lonicerae Japonicae, a typical medicinal flower, is widely used in China, Korea, and other Southeast Asian countries. However, Flos Lonicerae Japonicae and its closely related species have been misused and traded at varying for a wide range of prices. Therefore, Flos Lonicerae Japonicae must be accurately identified. In this study, the ITS2 and psbA-trnH regions were amplified by polymerase chain reaction (PCR). Sequence assembly was performed using CodonCode Aligner V 3.5.4. The intra- versus inter-specific variations were assessed using six metrics and “barcoding gaps.” Species identification was conducted using BLAST1 and neighbor-joining (NJ) trees. Results reveal that ITS2 and psbA-trnH exhibited an average intraspecific divergence of 0.001 and 0, respectively, as well as an average inter-specific divergence of 0.0331 and 0.0161. The identification efficiency of ITS2 and psbA-trnH evaluated using BLAST1 was 100%. Flos Lonicerae Japonicae was formed into one clade through the NJ trees. Therefore, Flos Lonicerae Japonicae can be stably and accurately identified through the ITS2 and psbA-trnH regions, respectively.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Stability and Accuracy Assessment of Identification of Traditional Chinese Materia Medica Using DNA Barcoding: A Case Study on Flos Lonicerae Japonicae

Hou

Song

Shi

et al. 2013

BioMed Research International

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“…DNA sequences have been extensively used in systematic taxonomic research for species identification [40,41]. DNA barcoding technology has been increasingly applied in authenticating herbal tea [42,43]. Stoeckle et al [42] used rbcL or matK barcodes to test the ingredients of 146 commercial tea products and showed that a third of the identified DNA was not found on herbal tea labels.…”

Section: Dna Barcoding Identification Of Herbal Teamentioning

confidence: 99%

“…Stoeckle et al [42] used rbcL or matK barcodes to test the ingredients of 146 commercial tea products and showed that a third of the identified DNA was not found on herbal tea labels. Li et al [43] systematically collected 177 samples of raw materials and established DNA barcodes for the identified botanical sources of Chinese "cooling" beverages. In this study, the CH-17 and CH-18 are commercial tea samples and high-quality DNA in both of them were extracted from these samples and the NJ tree and BLAST result based on similarity showed that they were identified as the labeled species.…”

Section: Dna Barcoding Identification Of Herbal Teamentioning

confidence: 99%

Identification of Traditional She Medicine Shi-Liang Tea Species and Closely Related Species Using the ITS2 Barcode

Zhou

et al. 2017

Applied Sciences

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Traditional She medicine is part of China's cultural heritage and has become remarkably popular worldwide. The Shi-Liang tea is made from the processed leaves of Chimonanthus salicifolius S. Y. Hu and Chimonanthus zhejiangensis M. C. Liu. To ensure the safety and efficacy of Shi-Liang tea, we used DNA barcoding based on the internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA to distinguish the original plant sources of Shi-Liang tea from closely related species. All 71 ITS2 sequences were aligned by Clustal-W, and genetic distances were computed using MEGA 6.0 according to the Kimura 2-parameter model. The results indicated that the sequence lengths of ITS2 regions of the original plants of Shi-Liang tea and closely related species ranged from 256 bp to 260 bp. Interspecific genetic distances ranged from 0 to 0.078. The neighbor-joining (NJ) tree showed that the original plants of Shi-Liang tea species can be easily differentiated from closely related species. Distinct molecular differences were found between the secondary structures of ITS2 sequences from Shi-Liang tea and closely related species. The results in the present investigation suggested that the ITS2 could be an effective DNA marker to identify the original plants of Shi-Liang tea and their closely related species. These DNA barcodes can greatly benefit the supervision of the commercial circulation of She medicine.

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“…Both consumers and foodstuff producers may take advantage of a DNA barcoding screening, especially concerning items distributed as shredded or powered material, which otherwise result as unidentifiable by a simple morphological analysis (Cornara et al, 2013). Among these, promising results were obtained in studies on commercial spices (De Mattia et al, 2011), herbal teas (Li et al, 2012) and fruit juices (Faria, Magalhães, Nunes, & Oliveira, 2013). Table 1 provides an updated list of case studies on identification and traceability of raw materials / processed foodstuffs by using DNA barcoding.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Emerging DNA-based technologies to characterize food ecosystems

Galimberti

Bruno

Mezzasalma

et al. 2015

Food Research International

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Establishment of DNA barcodes for the identification of the botanical sources of the Chinese ‘cooling’ beverage

Cited by 16 publications

References 31 publications

Stability and Accuracy Assessment of Identification of Traditional Chinese Materia Medica Using DNA Barcoding: A Case Study on Flos Lonicerae Japonicae

Stability and Accuracy Assessment of Identification of Traditional Chinese Materia Medica Using DNA Barcoding: A Case Study on Flos Lonicerae Japonicae

Identification of Traditional She Medicine Shi-Liang Tea Species and Closely Related Species Using the ITS2 Barcode

Emerging DNA-based technologies to characterize food ecosystems

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