Establishment of CD5 and CD10 double-positive mature B-cell line, WILL1, showing complex 8q24 translocation involving 14q32 and 6q27

Abstract

We established a novel mature B-cell line from a CD5 and CD10 double-positive diffuse large B-cell lymphoma patient, designated as WILL1. WILL1 cells were positive for CD5, CD10, CD19, and CD20. Spectral karyotype (SKY) analysis revealed chromosome 8 signals on 6q27 as well as 14q32. Fluorescence in situ hybridization (FISH) analysis suggested that a translocation break occurred outside the immunoglobulin heavy chain (IGH) gene on 14q32. Moreover, fusion signals of IGH and C-MYC probes were detected on the derivative 6 and derivative 14 chromosomes. Southern blot analysis using a C-MYC exon II fragment failed to detect rearrangement, suggesting that the 8q24 breakpoints lay far up- or downstream of the C-MYC gene. WILL1 is a useful tool to analyze the pathogenesis of CD5 and CD10 double-positive diffuse large B-cell lymphoma, and for molecular cloning of the unique translocation breakpoints of 14q32 and 8q24 and a novel gene on 6q27.

Bone marrow large B cell lymphoma bearing cyclin D3 expression: clinical, morphologic, immunophenotypic, and genotypic analyses of seven patients

“…Southern blot assays for class switch recombination were performed for patients 1 and 2, and a cell line established from patient 7, WILL1 [18]. The Southern blot assay can detect the vast majority of rearrangements of switch regions [19].…”

“…In patient 1, C-MYC rearrangements were detected by Southern blot analysis, although C-MYC translocation was undefined by conventional chromosomal analysis [3]. In patient 7, t(8;14)(q24;q32) was identified by fluorescence in situ hybridization using C-MYC and IGH probes [18]. Six patients excluding patient 6 showed the infiltrative or focal proliferation of morphologically abnormal lymphocytes.…”