Establishment of Azospirillum inoculant in the rhizosphere of winter wheat

Harris, J.; Lucas, John A.; Davey, M. R.; Lethbridge, G.; Powell, K.A.

doi:10.1016/0038-0717(89)90011-4

Cited by 33 publications

(10 citation statements)

References 16 publications

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“…On the one hand, many reports showed Azospirillum strains remaining at a high level for several months in the rhizosphere of the inoculated plant [22, 34–36]. On the other, it has been shown that Azospirillum is a poor colonist of cereal rhizosphere when introduced into soils under temperate conditions [37]. In our experiment, the sharp decrease of bacterial cells was probably due to drought conditions.…”

Section: Discussionsupporting

confidence: 51%

Development of a strain-specific probe to follow inoculated Azospirillum lipoferum CRT1 under field conditions and enhancement of maize root development by inoculation

Jacoud

Faure

Wadoux³

et al. 1998

FEMS Microbiology Ecology

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In order to develop a reliable and specific tool for the detection of Azospirillum lipoferum CRT1, randomly cloned DNA fragments from this strain were used as hybridization probes to differentiate A. lipoferum CRT1 from 29 closely related Azospirillum strains. Two cloned fragments hybridizing only with DNA from A. lipoferum CRT1 (CRT1‐5 and CRT1‐7) were considered as specific probes of this strain. CRT1‐7 fragment (1.4 kb) was further tested for purity control of the inoculant Azogreen‐m by colony hybridization. The sequence of the CRT1‐7 fragment has been determined and compared with those present in databases: no significant similarity with other sequences was detected. This probe permitted us to count specifically A. lipoferum CRT1 cells on maize roots during a field trial. During the first two weeks, A. lipoferum CRT1 remained at 107 CFU plant−1. Afterwards, bacterial concentration sharply decreased. We could not detect any CRT1 cells on maize roots 28 days after sowing. Concurrently, three plant parameters were estimated (plant height, primary root length and root fresh weight). The results showed that A. lipoferum CRT1 growth promotion effect began early on (from day 14) in plant development and increased in spite of a rapid decrease of bacterial density.

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Section: Discussionsupporting

confidence: 51%

Development of a strain-specific probe to follow inoculated Azospirillum lipoferum CRT1 under field conditions and enhancement of maize root development by inoculation

Jacoud

Faure

Wadoux³

et al. 1998

FEMS Microbiology Ecology

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“…Even moderate yield increases (up to 20%) attributed to inoculation with Azospirillum are considered commercially valuable to modern agriculture, if obtained consistently. However, the incidence of positive results may not be frequent enough to enable commercialization of the bacterial preparation; negative or no-effect results of inoculation were rarely reported (Albrecht et al 1981;Harris et al 1989;Smith et al 1984aSmith et al , 1984. The reports of very high yield responses were frequently due to low-yielding control plots, resulting from undetermined field conditions (Jagnow 1987).…”

Section: Effect Of Azospirillum Inoculation On Plantsmentioning

confidence: 99%

Current status of Azospirillum inoculation technology: Azospirillum as a challenge for agriculture

Bashan¹,

Levanony²

1990

Can. J. Microbiol.

350

187

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“…Commercial Azospirillum sp. inoculants are rare (Fages 1992) and it is difficult to evaluate whether some Azospirillum strains may exhibit harmful effects on plant growth in the absence of any significant published literature describing negative results (Harris et. al.…”

Section: Introductionmentioning

confidence: 99%

Azospirillum plant growth-promoting strains are nonpathogenic on tomato, pepper, cotton, and wheat

Bashan¹

1998

Can. J. Microbiol.

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Six strains of Azospirillum belonging to five species of plant growth-promoting bacteria (A. brasilense, A. lipoferum, A. amazonense, A. irakense, and A. halopraeference) did not cause visible disease symptoms on the roots or leaves of tomato, pepper, cotton, and wheat, failed to inhibit seed germination, and did not reduce plant dry weight when seven standard techniques for the inoculation of plant pathogens were used. Similar inoculation conditions with plant pathogens (Pseudomonas syringae pv. tomato, Xanthomonas campestris pv. vesicatoria, Xanthomonas campestris pv. translucens, and Xanthomonas campestris pv. malvacearum) induced typical disease symptoms. None of Azospirillum strains caused the hypersensitive reaction on eggplant, whereas all pathogens did. All Azospirillum strains increased phytoalexin production in all disease-resistant plant species to moderate levels, but the levels were significantly lower than those induced by the compatible pathogens. The various phytoalexins produced in plants had the capacity to inhibit growth of all Azospirillum strains. Azospirillum amazonense, A. irakense, and A. halopraeference had no apparent effect on plant growth, while A. brasilense and A. lipoferum increased the dry weight of all plant species. Under partial mist conditions, all Azospirillum strains were capable of colonizing leaf surfaces (10 3 -10 7 cfu/g dry weight) regardless of the plant species. These results provide experimental evidence that Azospirillum sp. might be considered safe for the inoculation of several plant species.Résumé : Six souches d'Azospirillum appartenant à cinq espèces de bactéries promotrices de croissance chez les plantes soit: A. brasilense, A. lipoferum, A. amazonense, A. irakense et A. halopraeference, n'ont pas causé de symptômes de maladies sur les racines ou les feuilles de la tomate, du piment, du coton et du blé, n'ont pas réussi à inhiber la germination des semences et n'ont pas réduit le poids sec des plantes, suite à l'utilisation de sept méthodes standard d'inoculation des agents phytopathogènes. Des conditions similaires d'inoculation avec des agents phytopathogènes, dont les Pseudomonas syringae pv. tomato, Xanthomonas campestris pv. vesicatoria, Xanthomonas campestris pv. translucens, Xanthomonas campestris pv. malvacearum, n'ont induit aucun symptôme typique de maladie. Aucune des souches d'Azospirillum n'a causé des réactions d'hypersensibilité chez l'aubergine, alors que tous les agents phytopathogènes y sont parvenu. Toutes les souches d'Azospirillum ont favorisé la production de phytoalexine chez toutes les espèces végétales résistantes aux maladies, à des niveaux modérés mais significativement inférieurs aux niveaux induits par les agents pathogènes compatibles. Les différentes phytoalexines produites chez les plantes étaient capables d'inhiber la croissance de toutes les souches d'Azospirillum. Les espèces A. amazonense, A. irakense et A. halopraeference, n'ont eu aucun effet apparent sur la croissance des plantes, tandis que l'A. brasilense et l'A. lipof...

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Establishment of Azospirillum inoculant in the rhizosphere of winter wheat

Cited by 33 publications

References 16 publications

Development of a strain-specific probe to follow inoculated Azospirillum lipoferum CRT1 under field conditions and enhancement of maize root development by inoculation

Development of a strain-specific probe to follow inoculated Azospirillum lipoferum CRT1 under field conditions and enhancement of maize root development by inoculation

Current status of Azospirillum inoculation technology: Azospirillum as a challenge for agriculture

Azospirillum plant growth-promoting strains are nonpathogenic on tomato, pepper, cotton, and wheat

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