Abstract:The prevalence of hepatitis B virus (HBV) infection in Indonesia was moderate in 2013. This makes an appropriate action has to be taken immediately. In an attempt to evaluate our candidate HBV vaccine efficacy, we generated monoclonal antibodies specific to HBcAg for sandwich ELISA application for research purposes. Monoclonal antibodies are generally developed using hybridoma technology by isolating B cells from spleen or lymph nodes followed by fusion with myeloma cells. This study generated hybridoma by mod… Show more
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