Establishment of an orthotopic tumour model for hepatocellular carcinoma and non-invasive in vivo tumour imaging by high resolution ultrasound in mice

Targeting heat-shock protein 90 improves efficacy of rapamycin in a model of hepatocellular carcinoma in mice

“…An orthotopic syngenic tumor model was used as described. 22 Briefly, 5 ϫ 10 4 Hepa129 cells were injected into the liver lobe of C3H mice. Treatment was initiated on day 4 after tumor cell implantation, with mice receiving: (1) 17-DMAG 25 mg/ kg, 3ϫ/wk, (2) rapamycin 0.2 mg/kg/day, (3) combination therapy, or (4) diluent.…”

“…Therefore, we validated our results in a C3H syngenic orthotopic tumor model using Hepa129 cells. 22 Because this tumor model elicits an aggressive growth behavior, we stepped up the treatment schedule by administering 17-DMAG three times per week (25 mg/kg) and rapamycin at 0.2 mg/kg/day. Single therapy with either 17-DMAG or rapamycin had no effect on hepatic tumor incidence (Fig.…”

Reconstitution of Secreted Frizzled-Related Protein 1 Suppresses Tumor Growth and Lung Metastasis in an Orthotopic Model of Hepatocellular Carcinoma

“…All experimental procedures were carried out according to the regulations and internal biosafety and bioethics guidelines of Harbin Medical University. Experimental orthotopic HCC was induced as described previously [18]. Briefly, the mice were randomly divided into three groups (n = 10 in each group) to receive subcapsular injection of 2 9 10 6 mock, SC-1 or SC-2 cells per mouse into the left liver lobe.…”

1,2-Dioleoyl-3-Trimethylammonium-Propane (DOTAP)-Formulated, Immune-Stimulatory Vascular Endothelial Growth Factor A Small Interfering RNA (siRNA) Increases Antitumoral Efficacy in Murine Orthotopic Hepatocellular Carcinoma with Liver Fibrosis

“…Usually, experimental antitumor strategies are tested in subcutaneous, but rarely in orthotopic, hepatoma models (29,30). Previously, the Hepa129 hepatoma cell line has been demonstrated to be suitable for orthotopic tumor growth and also for therapeutic intervention studies (29,31). Furthermore, our previous data demonstrated that liver fibrosis had a strong impact on orthotopic HCC growth.…”