Establishment of an isotope dilution LC-MS/MS method revealing kinetics and distribution of co-occurring mycotoxins in rats

Abstract

(2012). Establishment of an isotope dilution LC-MS/MS method revealing kinetics and distribution of co-occurring mycotoxins in rat. Analytical Methods, 4 (11), 3708-3717. 10.1039/c2ay25891a Establishment of an isotope dilution LC-MS/MS method revealing kinetics and distribution of co-occurring mycotoxins in rat AbstractAn isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) method with a fast sample preparation using homemade clean-up cartridges was developed for simultaneous determination of co-occurring mycotoxins exemplified with aflatoxin B1(AFB1) and T-2 toxin (T-2) in representative biomatrices of rat plasma, heart, liver, kidney, spleen, lung and brain in a total run time of 7 min. The established approach using stable

Liquid chromatography – high resolution mass spectrometry method for monitoring of 17 mycotoxins in human plasma for exposure studies

“…This is significantly better than reported LLOQs in literature for animal plasma matrices which ranged from 1 ng/mL for pig plasma [61], 2.5 ng/mL for chicken plasma [61] and 1-2 ng/mL for pig plasma [62]. Our results are comparable to analyte-specific method for AFB1 and T-2 where LOQ of 0.05 ng/mL was obtained in rat plasma after protein precipitation and SPE and using S/N criterion of 10 [16].Type B trichothecenes had LLOQs of 0.2 ng/mL (3AcDON and 15AcDON) and 0.3 ng/mL (DON) in our study. In contrast, Broakert et al reported LLOQs for 1-2 ng/mL for chicken plasma and 0.1-1 ng/mL for pig plasma for the same three analytes when using acetonitrile protein precipitation [63], de Baere et al reported 1 ng/mL for pig plasma (DON), 1.25 ng/mL for chicken plasma for DON when using a combination of protein precipitation and HLB SPE [61], and Brezina et al reported 0.45 ng/mL using HLB SPE for DON [41].…”

“…For aflatoxin B1 (AFB1), analysis of plasma also reflects medium-term exposure due to AFB1 binding to plasma albumin [14]. On the other hand, monitoring of mycotoxins such as fumonisin B1 (FB1) and T-2 toxin (T-2) in plasma provides evidence of short-term exposure due to their shorter lifetimes of 18 min and 8.1 hour in rat blood, respectively [15] [16].…”

Predicting the Acute Liver Toxicity of Aflatoxin B1 in Rats and Humans by an In Vitro–In Silico Testing Strategy

“…In rats, the predicted C max (10.08 ng mL −1 ) was only 1.01-fold higher than the C max in blood upon exposure of rats to 0.5 mg kg −1 bw. [47] Also, the predicted T max , the time to reach the C max , of 20 min, is close to the value reported by Han et al [47] of 10.2 min (0.17 h). For the human model, the C max for the 4 human volunteers reported by Jubert et al [15] was about 1.2-2.1fold higher than the C max predicted (0.39 pg mL −1 ).…”

“…To evaluate the performance of the PBK model, AFB1 concentrations in human or rat plasma reported in in vivo studies available in the literature [15,47] were transformed to concentrations in whole blood assuming that blood concentrations are 0.6 and 0.55 times the plasma concentrations in rats and humans. This enabled comparison to the PBK model predictions for blood concentrations (CB).…”

“…In rats, in vivo kinetic data for AFB1 levels in plasma after oral exposure were reported in two studies (Table S2, Supporting Information). Although in one of the studies, AFB1 was co-administered with 0.5 mg kg −1 bw T-2, [47] the data on AFB1 quantified in plasma by LC-MS were used for comparison assuming T-2 would not affect AFB1 bioavailability. The study by Coulombe Jr and Sharma, [14] used radiolabelled AFB1 and quantified the radioactivity, reporting the level of both AFB1 and its metabolites in plasma samples.…”