Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation

Lau, Skadi; Liu, Yue; Maier, Anna; Braune, S.; Gossen, Manfred; Neffe, Axel T.; Lendlein, Andreas

doi:10.1557/s43579-021-00072-6

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…[14] They are also an excellent material for optical components, as they are highly transparent, especially in the UV part of the spectrum. [13] Additionally, they are a good material for bioresearch due to their inertness and, as a result, are widely used in biological, [15][16][17][18] and membrane applications. [19][20][21] However, the inertness and low energy of the surface can also be detrimental to some biomedical applications, especially cell attachment.…”

Section: Introductionmentioning

confidence: 99%

Patterning of COC Polymers by Middle‐Energy Ion Beams for Selective Cell Adhesion in Microfluidic Devices

Aubrecht,

Malinský,

Novák

et al. 2024

Adv Materials Inter

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Microfluidic devices play a crucial role in advanced cell biology applications, including cell separations, cultivations, migration and interaction studies, diagnostic devices, and organ‐on‐chips. One of the frequent purposes of such devices is the ability to selectively address the attachment of cells at defined locations on the surface. This study explores the application of middle‐energy carbon, oxygen, and nitrogen ions to locally modify the surface of cyclic olefin copolymer (COC) thermoplastic material, allowing selective cell growth on patterned polymer surfaces. The investigation considers ion element type, ion beam energy, and ion irradiation fluence, analyzing their influence on the modification effect. Characterization of the modified surfaces involves various surface‐analytical methods such as contact angle, energy dispersive spectroscopy (SEM‐EDX), atomic force microscopy (AFM), x‐ray photoelectron spectroscopy (XPS), rutherford backscattering spectrometry (RBS), and elastic recoil detection analysis (ERDA). The study extends to practical aspects, with a representative cancer cell line, MCF‐7, grown on the patterned surface to evaluate the degree of selective attachment. Additionally, the stability of the irradiated patterns is tested under elevated temperatures beyond the glass transition temperature (Tg), demonstrating the compatibility of the approach with hot embossing technology. The findings underscore the potential of ion beam treatment for COC in cell‐biology‐related applications, offering insights into surface modification techniques for enhanced functionality in microfluidic devices.

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Section: Introductionmentioning

confidence: 99%

Patterning of COC Polymers by Middle‐Energy Ion Beams for Selective Cell Adhesion in Microfluidic Devices

Aubrecht,

Malinský,

Novák

et al. 2024

Adv Materials Inter

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“…Applications of COCs are manifold and range from materials for advanced packaging, [6,7] thermal insulation, [8] gas separation [9] to optical [10][11][12] and microfluidic devices for diverse applications [13][14][15][16] or artificial muscles [17] and optical biosensors. [18,19] Developing a full understanding of the characteristics of the glass transition behavior of these materials is important to their processing and use in a variety of those applications. [6] Developing a deeper insight into the glass transition characteristics of COCs is warranted.…”

Section: Introductionmentioning

confidence: 99%

Cyclic Olefin Copolymers (COC)—Excellent Glass Formers with Low Dynamic Fragility

Zhang

Vadlamudi

Shaffer

et al. 2022

Macro Chemistry & Physics

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Applications of cyclic olefin copolymers (COC) are manifold, and developing a complete understanding of the characteristics of the glass transition behavior of these materials is essential to their processing and use in a variety of those applications. Fast scanning calorimetry is employed to investigate the glass transition of three commercial TOPAS COCs of different norbornene content between 37 and 54 mol% at cooling rates varying from 0.01 to 100 000 K s −1 . The glass transition temperatures as a function of cooling rate are determined as limiting fictive temperatures from reheating scans at 100 000 K s −1 . The data, covering seven orders of magnitude in cooling rate, allows the determination of the dynamic fragilities. The dynamic fragilities of TOPAS 6013, 6015, and 8007 are in the range of 50-60 and increase with increasing norbornene content. Compared to literature data of other polymers, the dynamic fragilities are rather low, and the COCs are classified as relatively strong glass formers. In other words, relating this information to processability, they have a relatively large "working range" and are classified as "long" glasses. Such classification, borrowed from glass making (blowing) technologies, may also help to describe the processability of polymeric glass formers in general.

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Effect of Endothelial Culture Medium Composition on Platelet Responses to Polymeric Biomaterials

Lau

Maier

Braune

et al. 2021

IJMS

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Near-physiological in vitro thrombogenicity test systems for the evaluation of blood-contacting endothelialized biomaterials requires co-cultivation with platelets (PLT). However, the addition of PLT has led to unphysiological endothelial cell (EC) detachment in such in vitro systems. A possible cause for this phenomenon may be PLT activation triggered by the applied endothelial cell medium, which typically consists of basal medium (BM) and nine different supplements. To verify this hypothesis, the influence of BM and its supplements was systematically analyzed regarding PLT responses. For this, human platelet rich plasma (PRP) was mixed with BM, BM containing one of nine supplements, or with BM containing all supplements together. PLT adherence analysis was carried out in six-channel slides with plasma-treated cyclic olefin copolymer (COC) and poly(tetrafluoro ethylene) (PTFE, as a positive control) substrates as part of the six-channel slides in the absence of EC and under static conditions. PLT activation and aggregation were analyzed using light transmission aggregometry and flow cytometry (CD62P). Medium supplements had no effect on PLT activation and aggregation. In contrast, supplements differentially affected PLT adherence, however, in a polymer- and donor-dependent manner. Thus, the use of standard endothelial growth medium (BM + all supplements) maintains functionality of PLT under EC compatible conditions without masking the differences of PLT adherence on different polymeric substrates. These findings are important prerequisites for the establishment of a near-physiological in vitro thrombogenicity test system assessing polymer-based cardiovascular implant materials in contact with EC and PLT.

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Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation

Cited by 3 publications

References 29 publications

Patterning of COC Polymers by Middle‐Energy Ion Beams for Selective Cell Adhesion in Microfluidic Devices

Patterning of COC Polymers by Middle‐Energy Ion Beams for Selective Cell Adhesion in Microfluidic Devices

Cyclic Olefin Copolymers (COC)—Excellent Glass Formers with Low Dynamic Fragility

Effect of Endothelial Culture Medium Composition on Platelet Responses to Polymeric Biomaterials

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