Establishment of an ex vivo laticifer cell suspension culture from Taraxacum brevicorniculatum as a production system for cis-isoprene

Post, Janina; Eisenreich, Wolfgang; Huber, Claudia; Twyman, Richard M.; Prüfer, Dirk; Gronover, Christian Schulze

doi:10.1016/j.molcatb.2013.07.013

Cited by 9 publications

(3 citation statements)

References 61 publications

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“…However, at present, these experiments are restricted to Hevea brasiliensis , although it is expected that they can be applied in the future to T. kok‐saghyz and its close relative T. brevicorniculatum . Currently, many of the proteins and molecular pathways involved in rubber biosynthesis are known (Hillebrand et al, ; Post et al, ; Schmidt et al, ; Wahler et al, ). It was shown that rubber from T. kok‐saghyz contains less protein than rubber from H. brasiliensis , which might cause fewer allergic reactions (Van Beilen & Poirier, ).…”

Section: Cultivation Of Plants Of the Genus Taraxacummentioning

confidence: 99%

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Ethnobotany of the genus Taraxacum—Phytochemicals and antimicrobial activity

Sharifi‐Rad

Roberts

Matthews

et al. 2018

Phytotherapy Research

104

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Plants belonging to the genus Taraxacum have been used in traditional healthcare to treat infectious diseases including food-borne infections. This review aims to summarize the available information on Taraxacum spp., focusing on plant cultivation, ethnomedicinal uses, bioactive phytochemicals, and antimicrobial properties. Phytochemicals present in Taraxacum spp. include sesquiterpene lactones, such as taraxacin, mongolicumin B, and taraxinic acid derivatives; triterpenoids, such as taraxasterol, taraxerol, and officinatrione; and phenolic derivatives, such as hydroxycinnamic acids (chlorogenic, chicoric, and caffeoyltartaric acids), coumarins (aesculin and cichoriin), lignans (mongolicumin A), and taraxacosides. Aqueous and organic extracts of different plant parts exhibit promising in vitro antimicrobial activity relevant for controlling fungi and Gram-positive and Gram-negative bacteria. Therefore, this genus represents a potential source of bioactive phytochemicals with broad-spectrum antimicrobial activity. However, so far, preclinical evidence for these activities has not been fully substantiated by clinical studies. Indeed, clinical evidence for the activity of Taraxacum bioactive compounds is still scant, at least for infectious diseases, and there is limited information on oral bioavailability, pharmacological activities, and safety of Taraxacum products in humans, though their traditional uses would suggest that these plants are safe.

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Section: Cultivation Of Plants Of the Genus Taraxacummentioning

confidence: 99%

“…It is also possible to cultivate roots in a sterile environment controlling the growth conditions without phytohormones. Another possibility is to cultivate only the rubber‐producing laticifer cells in vitro; this has been tried as proof of concept but not at a larger scale (Post et al, ).…”

Section: Cultivation Of Plants Of the Genus Taraxacummentioning

confidence: 99%

Ethnobotany of the genus Taraxacum—Phytochemicals and antimicrobial activity

Sharifi‐Rad

Roberts

Matthews

et al. 2018

Phytotherapy Research

104

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“…The overexpression constructs for NtFT5 G170W and NtFT5 MM W170G were prepared by digesting the NtFT5 G170W and NtFT5 MM W170G amplicons (generated using the primers listed in Supplementary Table S1) with the appropriate restriction enzymes and inserting them into vector pRT104, containing the CaMV 35S promoter and terminator (Töpfer et al ., 1987). The entire expression cassette was then excised with HindIII and transferred to vector plab12.10 (Post et al ., 2014).…”

Section: Methodsmentioning

confidence: 99%

Unraveling the mystery behind the short-day-specific flowering of tobacco cultivar Maryland Mammoth

Zimmermann

Grundmann

Kaenel

et al. 2022

Preprint

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Flowering in day-neutral tobacco (Nicotiana tabacum) plants requires the photoperiod-dependent expression of members of the FLOWERING LOCUS T (FT) like clade of phosphatidylethanolamine-binding proteins. FT-like floral activators and inhibitors compete for interaction with FD proteins to shift from vegetative to reproductive growth. In the short-day (SD) cultivar Maryland Mammoth (MM), vegetative growth persists under long-day (LD) conditions, generating unusually tall plants. We found that the major floral inducer under long-days (NtFT5) was expressed in MM and that NtFT5 overexpression induced flowering in MM plants under LD conditions. However, sequence analysis revealed a 2-bp deletion near the 3′ end of NtFT5 in MM plants resulting in a truncated C-terminus with an altered amino acid sequence. We found that the truncated NtFT5MM protein was still able to interact with tobacco FD proteins. However, constitutive overexpression under LD conditions in SD-specific flowering tobacco plants showed that NtFT5MM is a weaker floral inducer than NtFT5. Our data indicate that the truncation does not impair the stability of the NtFT5MM protein but affects its binding affinity for NtFD1, probably resulting in the weaker expression of target genes. Our results therefore provide a potential explanation for the MM gigantism phenotype first observed more than 100 years ago.

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Secondary Metabolism in Tissue and Organ Cultures of Plants from the Tribe Cichorieae

Stojakowska

Malarz

2019

Reference Series in Phytochemistry

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Establishment of an ex vivo laticifer cell suspension culture from Taraxacum brevicorniculatum as a production system for cis-isoprene

Cited by 9 publications

References 61 publications

Ethnobotany of the genus Taraxacum—Phytochemicals and antimicrobial activity

Ethnobotany of the genus Taraxacum—Phytochemicals and antimicrobial activity

Unraveling the mystery behind the short-day-specific flowering of tobacco cultivar Maryland Mammoth

Secondary Metabolism in Tissue and Organ Cultures of Plants from the Tribe Cichorieae

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