Establishment of an Evaluation Model for Human Milk Fat Substitutes

Abstract

Fatty acid composition and distribution of human milk fat (HMF), from mothers over different lactating periods in Guangzhou, China, were analyzed. The universal characteristics were consistent with previously reported results although the fatty acid content was within a different range and dependent on the local population (low saturated fatty acid and high oleic acid for Guangdong mothers' milk fat). Based on the composition of the total and sn-2 fatty acids of mature milk fat, an efficient evaluation model was innovatively established by adopting the "deducting score" principle. The model showed good agreement between the scores and the degree of similarity by assessing 15 samples from different sources including four samples of HMF, eight samples of human milk fat substitutes (HMFSs) and infant formulas, and three samples of fats and oils. This study would allow for the devolvement of individual human milk fat substitutes with different and specific fatty acid compositions for local infants.

Production of human milk fat substitute with medium-chain fatty acids by lipase-catalyzed acidolysis: Optimization by response surface methodology

“…At the optimal reaction condition, the palmitic acid (30 g/100 g) content of the product was found to be compatible with the values of the colostrum milk (21.30e29.71 g/100 g) reported by Wang et al (2010).It can be interpreted that the SL produced in this study mimics human milk fat composition with respect to palmitic acid content.…”

Optimal Production and Biochemical Properties of a Lipase from Candida albicans

“…The hexane layer was dried over anhydrous sodium sulfate, and the solvent was removed with a rotary evaporator at 40 °C. Free fatty acids were methylated and analyzed by GC [40]. The dacylglycerols in the reaction mixture were analyzed by HPLC [41].…”

Production and immobilization of lipase PCL and its application in synthesis of α-linolenic acid-rich diacylglycerol

“…Before the analysis by GC, separation of different compositions in the reaction mixture was carried out using thin‐layer chromatography method according to Li et al (). Then, the scraped DAG, MAG, and the unreacted substrate (FA) were separately methylated to FAME according to the method described by Wang et al (). Finally, the analysis of the FA composition was performed using GC (Agilent 7890A) equipped with a capillary column CP‐Sil 88 (60m × 0.25mm × 0.2 μm) according to the method of Qin, Huang, Lan, Wang, and Yang ().…”