Establishment of an efficient transformation and CRISPR/Cas9-mediated gene editing system in Chinese local planting cassava (Manihot esculenta Crantz) cultivar SC8
Abstract:Cassava starch is a widely used raw materials for industrial production. South Chinese cassava cultivar 8 (Manihot esculenta Crantz cv. SC8) is one of the main locally planted cultivars. In this study, an efficient transformation system for cassava SC8 mediated with Agrobacterium strain LBA4404 has been presented for the first time, in which the factors of Agrobacterium strain cell infection (density OD600 = 0.65), 0.25 mM acetosyringone induction, and agro-cultivation with wet friable embryogenic callus (FEC)… Show more
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