Establishment of an Agrobacteriuim-mediated cotyledon disc transformation method for Jatropha curcas

Li, Meiru; Li, Hongqing; Jiang, Huawu; Pan, Xiaoping; Wu, Guojiang

doi:10.1007/s11240-007-9320-6

Cited by 120 publications

(80 citation statements)

References 25 publications

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“…Methods for reliable genetic transformation using Agrobacterium have been developed for switchgrass, Jatropha, poplar and Brachypodium [9][10][11][12], paving the way for genetic engineering approaches to crop improvement. For example, the successful engineering of a functional metabolic pathway for the production of polyhydroxybutyrate (PHB) in transgenic switchgrass has been recently reported, suggesting that complex traits can be engineered in this dedicated biofuel crop [13 ].…”

Section: Biofuel Cropsmentioning

confidence: 99%

Genetic and biotechnological approaches for biofuel crop improvement

Vega‐Sánchez

Ronald

2010

Current Opinion in Biotechnology

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Research and development efforts for biofuel production are targeted at converting plant biomass into renewable liquid fuels. Major obstacles for biofuel production include lack of biofuel crop domestication, low oil yields from crop plants as well as recalcitrance of lignocellulose to chemical and enzymatic breakdown. Researchers are expanding the genetic and genomic resources available for crop improvement, elucidating lipid metabolism to facilitate manipulation of fatty acid biosynthetic pathways and studying how plant cell walls are synthesized and assembled. This knowledge will be used to produce the next generation of biofuel crops by increasing fatty acid content and by optimizing the hydrolysis of plant cell walls to release fermentable sugars. Introduction Biofuels are commonly defined as fuels derived from renewable biological products and are often regarded as an attractive, 'green' alternative to fossil sources of energy due to their potential contribution to lowering carbon dioxide emissions [1]. Globally, plants produce an estimated 200 billion tones of biomass per year [2] in the form of sugars, polysaccharides, oils and other biopolymers, representing an unprecedented resource for biofuel production. However, despite its abundance and potential environmental benefits, the efficient and sustainable use of plant biomass for energy purposes remains a challenging endeavor, requiring major investments in science and technology [3,4].With the exception of sugar cane ethanol, biofuels are a nascent industry in many parts of the world. A few of the commercialized products include bioethanol derived from corn starch and biodiesel obtained from plants with a high content in fatty acids such as soybean, canola and sunflower ( Figure 1). However, the status of corn and soybean as major food crops, coupled to the fact that yields of starch and plant oil are too modest to cover the huge demand of transportation fuels has prompted the development of alternative biofuel production based on lignocellulosic biomass [4][5][6]. Lignocellulose, composed of the polysaccharides cellulose and hemicellulose, and lignin, a phenolic polymer, is the most abundant biomaterial on earth [2,7]. Most lignocellulosic feedstocks in consideration are perennial, non-food grasses such as switchgrass and Miscanthus, as well as woody plants such as poplar (Table 1).None of the current and potential crops has been domesticated or bred for improved polysaccharide or oil extraction for biofuel production. For this reason, biofuel research is focused towards understanding the plant biomass characteristics and traits that need to be modified to optimize crops for biofuel production. The wealth of genetic and genomic resources in model plants such as rice, Arabidopsis and Brachypodium are being used to answer fundamental scientific questions that cannot be addressed directly using potential biofuel crops. This review will discuss the recent advances in understanding lignocellulosic biomass recalcitrance and lipid metabolism for increasing oi...

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Section: Biofuel Cropsmentioning

confidence: 99%

Genetic and biotechnological approaches for biofuel crop improvement

Vega‐Sánchez

Ronald

2010

Current Opinion in Biotechnology

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“…Thus, the regeneration conditions described here are more e cient than those described in previous studies and can be applicable for e cient regeneration of transgenic plants from cotyledons and young leaves. Furthermore, it should be noted that our modi ed transformation system was applicable for three independent jatropha lines (from Philippines, ailand, and Tanzania; see Figure 2), whereas other systems (Kumar et al 2010;Li et al 2008) are applicable only to the line that they speci ed.…”

Section: Improvement Of the Transformation Protocol For Generating Trmentioning

confidence: 99%

“…One of the key challenges in successfully obtaining transgenic jatropha plants is the improvement of transformation e ciency. Initial transformation experiments conducted by our group employed the method of Li et al (2008) wherein tissue explants are simply incubated with the Agrobacterium cell suspension. Unfortunately this procedure produces extremely low transformation e ciency.…”

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confidence: 99%

Development of transgenic plants in jatropha with drought tolerance

Tsuchimoto

Cartagena

Khemkladngoen

et al. 2012

Plant Biotechnology

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Abstracte seed oil of jatropha (Jatropha curcas L.) is a source of biodiesel fuel. Although jatropha can grow in semiarid lands unsuitable for the food production, its oil productivity in such conditions is unsatisfactory at present. erefore, it is desirable to improve the oil productivity of jatropha even in semi-arid lands by enhancing its drought tolerance. Genetic engineering is promising to dramatically improve plant traits. Although we previously reported a transformation method, which involves wounding of tissue explants in order to increase the chance of Agrobacterium infection, for jatropha, it remains a challenge to enhance the shoot regeneration and root induction processes. Here, we report the generation of three kinds of transgenic jatropha plants in an attempt to improve their drought tolerance. e rst one overexpresses the PPAT gene, which encodes an enzyme that catalyzes the CoA biosynthetic pathway; the second overexpresses the NF-YB gene, which encodes a subunit of the NF-Y transcription factor; and the last overexpresses the GSMT and DMT genes, which encode enzymes that catalyze production of glycine betaine. We also report a modi ed protocol that improves the e ciency of shoot regeneration and root induction in transgenic jatropha plantlets.

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“…Due to its relatively small genome (2C value of 0.85 pg, in the same size range as that of rice) (Carvalho et al, 2008), Jatropha could become a model woody crop for biodiesel production. Genetic and genomic resources for this emerging biofuels crop are becoming available including a transformation system (Li et al, 2008), a 100x coverage of the J. curcas genome sequence (http://www.lifetechnologies.com/news-gallery/pressreleases/2010/life-techologies-ad-sg-biofuels-complete-sequece-of-jatropha-geo.html), and a growing library of expressed sequence tags (ESTs) from developing and germinating Jatropha seeds (Costa et al, 2010).…”

Section: Land-based Biofuel Crops With High Wue and Drought Tolerancementioning

confidence: 99%