Establishment of an Adult Medaka Fatty Liver Model by Administration of a Gubra-Amylin-Nonalcoholic Steatohepatitis Diet Containing High Levels of Palmitic Acid and Fructose

Abstract

Among lifestyle-related diseases, fatty liver is the most common liver disease. To date, mammalian models have been used to develop methods for inhibiting fatty liver progression; however, new, more efficient models are expected. This study investigated the creation of a new model to produce fatty liver more efficiently than the high-fat diet medaka model that has been used to date. We compared the GAN (Gubra-Amylin nonalcoholic steatohepatitis) diet, which has been used in recent years to induce fatty liver in mice, and the high-fat diet (HFD). Following administration of the diets for three months, enlarged livers and pronounced fat accumulation was noted. The GAN group had large fat vacuoles and lesions, including ballooning, compared to the HFD group. The GAN group had a higher incidence of lesions. When fenofibrate was administered to the fatty liver model created via GAN administration and liver steatosis was assessed, a reduction in liver fat deposition was observed, and this model was shown to be useful in drug evaluations involving fatty liver. The medaka fatty liver model administered with GAN will be useful in future fatty liver research.

The AMPK and AKT/GSK3β pathways are involved in recombinant proteins fibroblast growth factor 1 (rFGF1 and rFGF1a) improving glycolipid metabolism in rainbow trout (Oncorhynchus mykiss) fed a high carbohydrate diet

“…Histological features were observed and photographed with an optical microscope (Nikon Ds-Ri2, Japan). Evaluation of liver features primarily involved observing and analyzing pathological characteristics such as liver vacuolization, lipid droplet count, and inflammatory cell infiltration in pathological sections ( Fujisawa et al., 2021 ; Yu et al., 2021 ).…”

Amino acid metabolomics and machine learning for assessment of post-hepatectomy liver regeneration

“…The healthy group was given a standard diet for 12 weeks. The NASH group was given the Gubra-Amylin-NASH diet (D09100310, 40% fat, 22% fructose, and 2% cholesterol) for 12 weeks (Boland et al, 2019;Fujisawa et al, 2021). Then, the left and middle lobes of the liver were resected according to our previous studies (Lei et al, 2022;Dai et al, 2023;Wang et al, 2023).…”

Innovative Molecular Target and Therapeutic Approaches in Nonalcoholic Fatty Liver Disease/Nonalcoholic Steatohepatitis (NAFLD/NASH) 2.0

“…According to recent reports, the methionine and choline-deficient (MCD) diet remains one of the most effective in inducing NAFLD/NASH [ 3 ]. Innovative experimental models, such as the use of adult medaka strain, administered with Gubra-Amylin nonalcoholic steatohepatitis (GAN), have been proposed as an efficient fatty liver model [ 4 ].…”