Establishment of Agrobacterium tumefaciens- Mediated Transformation System for Rice Sheath Blight Pathogen Rhizoctonia solani

Yang, Yalin; Yang, Mei; Li, Minghai; Li, Yong; XiaoXia, He; Zhou, Erxun

doi:10.1016/s1672-6308(12)60008-0

Cited by 11 publications

(7 citation statements)

References 17 publications

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“…Further work is required to overcome this limitation which may be possible since transformation methods have been published for other R . solani AGs [ 54 , 55 ]. However, based on results obtained in the current study, possible targets for antagonistic measures already can be suggested such as blocking of initial signaling cascades and/or fungal differentiation processes.…”

Section: Discussionmentioning

confidence: 99%

The Rhizoctonia solani AG1-IB (isolate 7/3/14) transcriptome during interaction with the host plant lettuce (Lactuca sativa L.)

et al. 2017

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The necrotrophic pathogen Rhizoctonia solani is one of the most economically important soil-borne pathogens of crop plants. Isolates of R. solani AG1-IB are the major pathogens responsible for bottom-rot of lettuce (Lactuca sativa L.) and are also responsible for diseases in other plant species. Currently, there is lack of information regarding the molecular responses in R. solani during the pathogenic interaction between the necrotrophic soil-borne pathogen and its host plant. The genome of R. solani AG1-IB (isolate 7/3/14) was recently established to obtain insights into its putative pathogenicity determinants. In this study, the transcriptional activity of R. solani AG1-IB was followed during the course of its pathogenic interaction with the host plant lettuce under controlled conditions. Based on visual observations, three distinct pathogen-host interaction zones on lettuce leaves were defined which covered different phases of disease progression on tissue inoculated with the AG1-IB (isolate 7/3/14). The zones were defined as: Zone 1—symptomless, Zone 2—light brown discoloration, and Zone 3—dark brown, necrotic lesions. Differences in R. solani hyphae structure in these three zones were investigated by microscopic observation. Transcriptional activity within these three interaction zones was used to represent the course of R. solani disease progression applying high-throughput RNA sequencing (RNA-Seq) analysis of samples collected from each Zone. The resulting three transcriptome data sets were analyzed for their highest expressed genes and for differentially transcribed genes between the respective interaction zones. Among the highest expressed genes was a group of not previously described genes which were transcribed exclusively during early stages of interaction, in Zones 1 and 2. Previously described importance of up-regulation in R. solani agglutinin genes during disease progression could be further confirmed; here, the corresponding genes exhibited extremely high transcription levels. Most differentially higher expressed transcripts were found within Zone 2. In Zone 3, the zone with the strongest degree of interaction, gene transcripts indicative of apoptotic activity were highly abundant. The transcriptome data presented in this work support previous models of the disease and interaction cycle of R. solani and lettuce and may influence effective techniques for control of this pathogen.

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Section: Discussionmentioning

confidence: 99%

The Rhizoctonia solani AG1-IB (isolate 7/3/14) transcriptome during interaction with the host plant lettuce (Lactuca sativa L.)

et al. 2017

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“…The high virulent isolate GD‐118 of R. solani AG‐1 IA used in this study was isolated from the diseased rice sheath with typical symptom of sheath blight collected from Guangdong province, China, and stored at our laboratory at South China Agricultural University (Zhou and Yang ; Yang et al. ,b, 2012b).…”

Section: Methodsmentioning

confidence: 99%

Survival of Rhizoctonia solani AG‐1 IA, the Causal Agent of Rice Sheath Blight, under Different Environmental Conditions

Feng

Shu

Wang

et al. 2016

Journal of Phytopathology

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aThe first two authors contributed equally to this work. AbstractThe ability of Rhizoctonia solani AG-1 IA, the causal agent of rice sheath blight, to survive in diseased rice straw and as sclerotia and mycelia was investigated. After storage for 10 months at 4°C, 25°C and non-air-conditioned natural room temperature (NRT, temperature range from 6°C to 35°C), sclerotia placed inside a desiccator, soaked in sterile water or immersed in wet paddy soil were viable. In contrast, only 15% of sclerotia in dry paddy soil survived. Survival of mycelia was severely affected by temperature and humidity. After 10 months in a desiccator at 4°C, 55% of mycelia samples could survive, whereas at 25°C and NRT, mycelial samples survived for only 7 and 5 months, respectively. However, mycelia stored in sterile water at constant temperatures (4°C or 25°C) survived for 10 months. A certain amount of UV radiation had no obvious effect on the survival of sclerotia or mycelia. The survival rate of the fungus in diseased rice straw stored for 16 months could reach 100% at 4°C, 50% at 25°C and 35% at NRT. The survival rates of the pathogen in diseased rice straw buried in dry, wet and flooded paddy soils after 10-month storage at NRT were 75, 100 and 100%, respectively, indicating that soil humidity is a crucial factor for the survival of this fungus.

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“…The transformation method was used with leaf segments as explants. The transgenic system for 'Hanfu' has been previously established (Yang et al, 2010). The system was as follows: a single colony from the bacterial strain was inoculated into liquid yeast extract peptone (YEP) medium supplemented with 25 mgÁL -1 kanamycin and 100 mgÁL -1 spectinomycin, and grown at 28°C for 16 h on an orbital shaker at 165 rpm.…”

Section: Methodsmentioning

confidence: 99%

Modification of Plant Height via RNAi Suppression of MdGA20-ox Gene Expression in Apple

Zhao

Zhang

Yang

et al. 2016

J. Amer. Soc. Hort. Sci.

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GA20-oxidase (GA20-ox) is a key enzyme involved in the biosynthesis of gibberellic acid (GA). To investigate its role in plant growth and development, we suppressed MdGA20-ox gene expression in apple (Malus domestica cv. Hanfu) plants by RNA interference (RNAi). After 20 weeks of growth in the greenhouse, significant phenotype differences were observed between transgenic lines and the nontransgenic control. Suppression of MdGA20-ox gene expression resulted in lower plant height, shorter internode length, and higher number of nodes compared with the nontransgenic control. The expression of MdGA20-ox in transgenic plants was significantly suppressed, and the active GA content in transgenic lines was lower than that in the nontransgenic control. These results demonstrated that the MdGA20-ox gene plays an important role in vegetative growth, and therefore it is possible to develop dwarfed or compact scion apple cultivars by MdGA20-ox gene silencing.

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Establishment of Agrobacterium tumefaciens- Mediated Transformation System for Rice Sheath Blight Pathogen Rhizoctonia solani

Cited by 11 publications

References 17 publications

The Rhizoctonia solani AG1-IB (isolate 7/3/14) transcriptome during interaction with the host plant lettuce (Lactuca sativa L.)

The Rhizoctonia solani AG1-IB (isolate 7/3/14) transcriptome during interaction with the host plant lettuce (Lactuca sativa L.)

Survival of Rhizoctonia solani AG‐1 IA, the Causal Agent of Rice Sheath Blight, under Different Environmental Conditions

Modification of Plant Height via RNAi Suppression of MdGA20-ox Gene Expression in Apple

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